Summary
Leaf explants of Sinningia speciosa were cultured in vitro on Murashige and Skoog (MS) basal medium with various growth substances in order to regenerate shoots. On MS medium supplemented with indoleacetic acid (IAA) and kinetin, 80% of the explants produced green callus and 25 to 30 shoots with roots per explant. On MS supplemented with IAA and N6 benzyladenine (BA), 80% of the explants produced green callus and 40 to 50 shoots per explant but lacked roots. After 3–4 mo., these shoots were removed from the initial explants and transferred separately onto MS supplemented with indolebutyric acid for their elongation and successive rooting (3 mo.). Histological studies showed that the callus was associated with mesophyll cell layers, primarily with the spongy parenchyma. The shoots regenerated at the callus surface and were associated with newly differentiated vascular areas. Recurrent regenerations were obtained from leaf explants or apical meristems excised from shoots of the previous subcultures. These explants, as compared to initial cultures, had a high frequency of regeneration and also produced more shoots per explant. Chromosome numbers of root tip cells of the mother plant and of all in vitro-regenerated plants remained constant: 2n=26.
Similar content being viewed by others
References
Battaglia, E. A new 5 minute fixation in chromosome analysis. Caryologia 9:368–370; 1957.
Bilkey, P. C. Micropropagation of African violet from petiole cross sections. HortScience 13:37–38; 1978.
Cooke, R. C. Tissue culture propagation of African violets. HortScience 12:549; 1977.
Darlington, C. D.; Wyle, A. P. Chromosome atlas of flowering plants. 2nd ed. London: G. Allen & UnWin Ltd.; 1955:317.
Haramaki, C. Tissue culture of Gloxinia. Int. Plant. Prop. Soc. 21:442–448; 1971 (in Johnson, 1978).
Johnson, B. B. In vitro propagation of Gloxinia from leaf explants. HortScience 13:149–150; 1978.
Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962.
Raman, K. Rapid multiplication of Streptocarpus and Gloxinia from in vitro cultured pedicel segments. Z. Pflanzenphysiol. 83:411–418; 1977.
Rao, A. N. In vitro culture of leaf fragments of Saintpaulia ionantha. La culture des tissues et des cellules des végétaux, travaux dédiés à la memoire de G. Morel. Paris: Masson Editeur; 1977:110–122.
Smith, R. H.; Norris, R. E. In vitro propagation of African violet chimeras. HortScience 18:436–437; 1983.
Start, N. D.; Cumming, B. G. In vitro propagation of Saintpaulia ionantha Wendl. HortScience 11:204–206; 1976.
Sugiura, T. Studies on the chromosome numbers in higher plants. Cytologia 7:547–549; 1936.
Welander, M. Plant regeneration from leaf and stem segments of shoots raised in vitro from mature apple trees. J. Plant Physiol. 132:738–744; 1988.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Scaramuzzi, F., Apollonio, G. & D’Emerico, S. Adventitious shoot regeneration from Sinningia speciosa leaf discs in vitro and stability of ploidy level in subcultures. In Vitro Cell.Dev.Biol.-Plant 35, 217–221 (1999). https://doi.org/10.1007/s11627-999-0081-2
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11627-999-0081-2