Summary
The efficient exchange of gases between roots and their environment is one of the biggest challenges in bioreactor design for transformed root cultures. Gas-phase reactors can alleviate this problem as well as provide a new tool for studying the biological response of roots and other differentiated tissues to changes in the gas phase composition. In our comparison of liquid- and gas-phase reactors, roots grown in liquid (shake flasks or bubble column reactors) are shown to be under hypoxic stress. Roots grown in a gas-phase reactor (nutrient mist), while not hypoxic, produced 50% less biomass. These results suggest that the response of the tissues to gas phase composition are complex and need further study.
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Weathers, P.J., Wyslouzil, B.E., Wobbe, K.K. et al. The biological response of hairy roots to O2 levels in bioreactors. In Vitro Cell.Dev.Biol.-Plant 35, 286–289 (1999). https://doi.org/10.1007/s11627-999-0035-8
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DOI: https://doi.org/10.1007/s11627-999-0035-8