Abstract
Tissue culturing is widely used for the clonal propagation of several plant species. However, high mortality rates may occur during the acclimatization phase due to the sudden, dramatic changes in light levels. This study evaluated the effect of light level during the acclimatization of Neoregelia concentrica (Vellozo) LB Smith using chlorophyll a fluorescence and growth analysis. N. concentrica plants were multiplied and rooted in vitro on Murashige and Skoog medium and then transferred to plastic trays with individual cells containing vermiculite. The treatments consisted of the ex vitro cultivation of plants under light levels of 30, 50, 70, and full sunlight (100% light) for 28 d. Chlorophyll a fluorescence measurements were taken at 0 (in vitro), 7, 14, 21, and 28 d of acclimatization, and growth trait analyses were made at 28 d. The high irradiance under full sunlight (100% light) induced photoinhibition and reduced the growth rate. Plants grown under partial light (30, 50, and 70%) were less inhibited. A 50% reduction in light level during N. concentrica acclimatization produced rapid plant recovery and higher growth rates than full sunlight.
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Adams WW, Miller O, Cohu CM, Demmig-Adams B (2013) May photoinhibition be a consequence, rather than a cause, of limited plant productivity? Photosynth Res 117:31–44
Adamski JM, Peters JA, Danieloski R, Bacarin MA (2011) Excess iron-induced changes in the photosynthetic characteristics of sweet potato. J Plant Physiol 168:2056–2062
Apóstolo N, Brutti C, Llorente B (2005) Leaf anatomy of Cynara scolymus L. in successive micropropagation stages. In Vitro Cell Dev Biol Plant 41:307–313
Aragón C, Carvalho L, González J, Escalona M, Amancio S (2012) The physiology of ex vitro pineapple (Ananas comosus L. Merr. var MD-2) as CAM or C3 is regulated by the environmental conditions. Plant Cell Rep 31:757–769
Araújo SAC, Deminicis BB (2009) Fotoinibição da fotossíntese. Rev Bras Biosci 7:463–472
Arigita L, Gonzalez A, Tamés RS (2002) Influence of CO2 and sucrose on photosynthesis and transpiration of Actinia deliciosa explants cultured in vitro. Physiol Plant 115:166–173
Aro EM, Virgin I, Andersson B (1993) Photoinhibition of photosystem II. Inactivation, protein damage and turnover. Biochim Biophys Acta 1143:113–134
Asada K (2006) Production and scavenging of reactive oxygen species in chloroplasts and their functions. Plant Physiol 141:391–396
Bolhar-Nordenkampf HR, Long SP, Baker NR, Öquist G, Schreiber U, Lechner G (1989) Chlorophyll fluorescence as a probe of the photosynthetic competence of leaves in the field: a review of current instrumentation. Funct Ecol 3:497–514
Brito G, Costa A, Coelho C, Santos C (2009) Large-scale field acclimatization of Olea maderensis micropropagated plants: morphological and physiological survey. Trees 23:1019–1031
Čaňová I, Ďurkovič J, Hladká D (2008) Stomatal and chlorophyll fluorescence characteristics in European beech cultivars during leaf development. Biol Plant 52:577–581
Carvalho ACPP, Santos EO, Rodrigues AAJ (2011) Panorama da produção de mudas micropropagadas no Brasil. In: Gerald LTS (ed) Biofábrica de plantas: produção industrial de plantas in vitro. Atiqua, São Paulo, pp 380–393
Carvalho LC, Osório ML, Chaves MM, Amâncio S (2001) Chlorophyll fluorescence as an indicator of photosynthetic functioning of in vitro grapevine and chestnut plantlets under ex vitro acclimatization. Plant Cell Tissue Organ Cult 67:271–280
Chu EP, Tavares AR, Kanashiro S, Giampaoli P, Yokota ES (2010) Effects of auxins on soluble carbohydrates, starch and soluble protein content in Aechmea blanchetiana (Bromeliaceae) cultured in vitro. Sci Hortic 125:451–455
Cruz CD (2013) Genes: a software package for analysis in experimental statistics and quantitative genetics. Acta Sci Agron 35:271–276
Demmig-Adams B, Adams WW (1992) Photoprotection and other responses of plants to high light stress. Annu Rev Plant Physiol 43:599–626
Dias DP, Marenco RA (2006) Photoinhibition of photosynthesis in Minquartia guianensis and Swietenia macrophylla inferred by monitoring the initial fluorescence. Photosynthetica 44:235–240
Droste A, Silva AM, Matos AV, Almeida JW (2005) In vitro culture of Vriesea gigantea and Vriesea philippocoburgii: two vulnerable bromeliads native to southern Brazil. Braz Arch Biol Technol 48:717–722
Ďurkovič J, Čaňová I, Pichler V (2009) Water loss and chlorophyll fluorescence during ex vitro acclimatization in micropropagated black mulberry (Morus nigra L.). Prop Ornam Plants 9:107–112
Guerra MP, Vesco LLD (2010) Strategies for the micropropagation of bromeliads. In: Jain SM, Ochatt SJ (eds) Protocols for in vitro propagation of ornamental plants: methods in molecular biology. Humana Press, New York, pp 47–66
Hazarika BN (2006) Morpho-physiological disorders in in vitro culture of plants. Sci Hortic 108:105–120
Huang PL, Liao LJ, Tsai CC, Liu ZH (2011a) Micropropagation of bromeliad Aechmea fasciata via floral organ segments and effects of acclimatization on plantlet growth. Plant Cell Tissue Organ Cult 105:73–78
Huang PL, Liu ZH, Chang ML, Liao LJ (2011b) Micropropagation of the bromeliad Guzmania ‘Hilda’ via organogenesis and the effect of α-naphthaleneacetic acid on plantlet elongation. Sci Hortic 130:894–898
Kitao M, Lei TT, Koike T, Tobita H, Maruyama Y (2000) Susceptibility to photoinhibition of three deciduous broadleaf tree species with different successional traits raised under various light regimes. Plant Cell Environ 23:81–89
Kozai T, Kubota C (2001) Developing a photoautotrophic micropropagation system for woody plants. J Plant Res 114:525–537
Martins JPR, Schimildt ER, Alexandre RS, Castro EM, Nani TF, Pires MF, Pasqual M (2014) Direct organogenesis and leaf-anatomy modifications in vitro of Neoregelia concentrica (Vellozo) L.B. Smith (Bromeliaceae). Pak J Bot 46:2179–2187
Martins JPR, Schimildt ER, Alexandre RS, Santos BR, Magevski GC (2013) Effect of synthetic auxins on in vitro and ex vitro bromeliad rooting. Pesq Agrop Trop 43:138–146
Mehta P, Jajoo A, Mathur S, Bharti S (2010) Chlorophyll a fluorescence study revealing effects of high salt stress on photosystem II in wheat leaves. Plant Physiol Biochem 48:16–20
Michael PI, Krishnaswamy M (2011) The effect of zinc stress combined with high irradiance stress on membrane damage and antioxidative response in bean seedlings. Environ Exp Bot 74:171–177
Mohamed AA (2008) Promotive effects of a 5-aminolevulinic acid-based fertilizer on growth of tissue culture-derived date palm plants (Phoenix dactylifera L.) during acclimatization. Sci Hortic 118:48–52
Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497
Negrelle RRB, Mitchell D, Anacleto A (2012) Bromeliad ornamental species: conservation issues and challenges related to commercialization. Acta Sci Biol Sci 34:91–100
Oliveira Y, Pinto F, Silva ALL, Guedes I, Biasi LA, Quoirin M (2010) An efficient protocol for micropropagation of Melaleuca alternifolia Cheel. In Vitro Cell Dev Biol Plant 46:192–197
Osório ML, Gonçalves S, Osório J, Romano A (2005) Effects of CO2 concentration on acclimatization and physiological responses of two cultivars of carob tree. Biol Plant 49:161–167
Osório ML, Osório J, Gonçalves S, David MM, Correia MJ, Romano A (2012) Carob trees (Ceratonia siliqua L.) regenerated in vitro can acclimatize successfully to match the field performance of seed-derived plants. Trees 26:1837–1846
Osório ML, Osório J, Romano A (2010) Chlorophyll fluorescence in micropropagated Rhododendron ponticum subsp. baeticum plants in response to different irradiances. Biol Plant 54:415–422
Oukarroum A, El Madidi S, Schansker G, Strasser RJ (2007) Probing the responses of barley cultivars (Hordeum vulgare L.) by chlorophyll a fluorescence OLKJIP under drought stress and re-watering. Environ Exp Bot 60:438–446
Palliotti A, Silvestroni O, Petoumenou D (2009) Photosynthetic and photoinhibition behavior of two field-grown grapevine cultivars under multiple summer stresses. Am J Enol Vitic 60:189–198
Pickens K, Wolf J, Affolter JM, Wetzstein HY (2006) Adventitious bud development and regeneration in Tillandsia eizii. In Vitro Cell Dev Biol Plant 42:348–353
Pospíšilová J, Synková H, Haisel D, Baťková P (2009) Effect of abscisic acid on photosynthetic parameters during ex vitro transfer of micropropagated tobacco plantlets. Biol Plant 53:11–20
Sáez PL, Bravo LA, Sáez KL, Sánchez-Olate M, Latsague MI, Ríos DG (2012) Photosynthetic and leaf anatomical characteristics of Castanea sativa: a comparison between in vitro and nursery plants. Biol Plant 56:15–24
Santos DS, Tamaki V, Nievola CC (2010) In vitro propagation of the ornamental bromeliad Acanthostachys strobilacea (Schult. f.) Klotzsch via nodal segments. In Vitro Cell Dev Biol Plant 46:524–529
Seon JH, Cui YY, Kozai T, Paek KY (2000) Influence of in vitro growth on photosynthetic competence and survival rate of Rehmannia glutinosa plantlets during acclimatization period. Plant Cell Tissue Organ Cult 61:135–142
Silva ALL, Costa JL, Alcantara GB, Carvalho DC, Schuck MR, Biasi LA, Scheidt GN, Soccol CR (2012) Micropropagation of Nidularium innocentii Lem. and Nidularium procerum Lindm (Bromeliaceae). Pak J Bot 44:1095–1101
Souza RP, Machado EC, Silva JAB, Lagoa AMMA, Silveira JAG (2004) Photosynthetic gas exchange, chlorophyll fluorescence and some associated metabolic changes in cowpea (Vigna unguiculata) during water stress and recovery. Environ Exp Bot 51:45–56
Strasser BJ, Strasser RJ (1995) Measuring fast fluorescence transients to address environ-mental questions: the JIP-test. In: Mathis P (ed) Photosynthesis: from light to biosphere. Kluwer Academic Publishers, Dordrecht, pp 977–980
Strasser RJ, Stirbet AD (1998) Heterogeneity of photosystem II probed by the numerically simulated chlorophyll a fluorescence rise (O–J–I–P). Math Comput Simul 48:3–9
Strasser RJ, Tsimilli-Michael M (2001) Stress in plants, from daily rhythm to global changes, detected and quantified by the JIP-test. Chim Nouv 75:3321–3326
Strasser RJ, Tsimilli-Michael M, Srivastava A (2004) Analysis of the chlorophyll a fluorescence transient. In: Papageorgiou GC, Govindjee (eds) Chlorophyll fluorescence: a signature of photosynthesis. Advances in photosynthesis and respiration series. Springer, Dordrecht, pp 321–362
Swain SS, Tripathy T, Mohapatra PK, Chand PK (2010) Photosynthetic and transpiration responses of in vitro-regenerated Solanum nigrum L. plants to ex vitro adaptation. In Vitro Cell Dev Biol Plant 46:134–141
Takahashi S, Badger MR (2011) Photoprotection in plants: a new light on photosystem II damage. Trends Plant Sci 16:53–60
Valladares F, Niinemets Ü (2008) Shade tolerance, a key plant feature of complex nature and consequences. Annu Rev Ecol Evol Syst 39:237–257
Van Heerden PDR, Swanepoel JW, Krüger GHJ (2007) Modulation of photosynthesis in two desert scrub species exhibiting C3-mode CO2 assimilation. Environ Exp Bot 61:124–136
Van Kooten O, Snel JFH (1990) The use of chlorophyll fluorescence and other noninvasive spectroscopic techniques in plant stress physiology. Photosynth Res 25:146–332
Yaseen M, Ahmad T, Sablok G, Standardi A, Hafiz IA (2013) Review: role of carbon sources for in vitro plant growth and development. Mol Biol Rep 40:2837–2849
Yusuf MM, Kumar D, Rajwanshi R, Strasser RJ, Tsimilli-Michael M, Govindjee, Sarin NB (2010) Overexpression of γ-tocopherol methyl transferase gene in transgenic Brassica juncea plants alleviates abiotic stress: physiological and chlorophyll fluorescence measurements. Biochim Biophys Acta 1797:1428–1438
Zhang F, Wang W, Ge Y, Shen X, Tian D, Liu J, Liu X, Yu X, Zhang Z (2012) Genetic relatedness among Aechmea species and hybrids inferred from AFLP markers and pedigree data. Sci Hortic 139:39–45
Acknowledgments
The authors would like to acknowledge the scholarship awarded by Espírito Santo State Research Foundation (FAPES). The authors are also grateful to Patrick Callegari Magnani for his technical assistance.
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Martins, J.P.R., Schimildt, E.R., Alexandre, R.S. et al. Chlorophyll a fluorescence and growth of Neoregelia concentrica (Bromeliaceae) during acclimatization in response to light levels. In Vitro Cell.Dev.Biol.-Plant 51, 471–481 (2015). https://doi.org/10.1007/s11627-015-9711-z
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DOI: https://doi.org/10.1007/s11627-015-9711-z