Abstract
MicroRNAs are a class of highly conserved and widely distributed non-coding RNAs. It is known that miR-26b has a high abundance in adipose tissue and is considered to be an effective regulator of adipogenesis. However, it is unclear whether miR-26b-5p, the product of miR-26b precursor, has the same effect as miR-26b. In the present study, we explored the potential role of miR-26b-5p in preadipocyte differentiation of goats. We found that the expression of miR-26b-5p had dramatic change during goat intramuscular preadipocyte differentiation. Transfection and RT-qPCR revealed that overexpression of miR-26b-5p increased the level of adipogenic marker genes and lipid accumulation in goat preadipocyte, suggesting that miR-26b-5p positively regulates goat preadipocyte differentiation. Furthermore, bioinformatics analysis and dual fluorescein reporter assays were performed to predict and validate the targets of miR-26b-5p. The results showed that miR-26b-5p has a binding site in the 3′UTR of FGF21 and overexpression of miR-26b-5p significantly down-regulated the expression of FGF21 mRNA. Luciferase activity assays confirmed that miR-26b-5p is a positive regulator of goat intramuscular preadipocyte via targeting FGF21. These findings provide reference for further revealing of the regulatory networks of goat fat metabolism and contribute to a better understanding of intramuscular fat deposition in goats.
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Acknowledgment
We are grateful to Dr. Bai Wenlin for proofreading this manuscript and modify the statement critically.
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This work was jointly supported by the National Key R&D Program of China (2018YFD0502002), the National Natural Science Foundation of China (31672395), the Applied Basic Research Program Key Project of Sichuan Province (2018JY0036), and the college students’ innovation project of Southwest Minzu University (CX2018SZ09).
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Editor: Tetsuji Okamoto
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Ma, J., Lin, Y., Zhu, J. et al. MiR-26b-5p regulates the preadipocyte differentiation by targeting FGF21 in goats. In Vitro Cell.Dev.Biol.-Animal 57, 257–263 (2021). https://doi.org/10.1007/s11626-020-00493-y
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DOI: https://doi.org/10.1007/s11626-020-00493-y