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LncRNA TUG1 promoted osteogenic differentiation through promoting bFGF ubiquitination

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Abstract

LncRNA TUG1 has the potential to promote the osteogenic differentiation of several cells, but the role of lncRNA TUG1 in osteogenic differentiation of tendon stem/progenitor cells (TSPCs) is still unknown. This study aims to determine the role of lncRNA TUG1 in osteogenic differentiation of TSPCs. bFGF, RUNX2, and Osterix protein expressions were detected by western blot. LncRNA TUG1 and bFGF expression was detected by qRT-PCR. RNA immunoprecipitation (RIP) assay was used to confirm the interaction between TUG1 and bFGF2. Ubiquitination assay was used to determine the ubiquitination of bFGF protein. During osteogenic differentiation, the protein expression of bFGF was significantly downregulated in TSPCs, and the expression of TUG1 was significantly elevated in TSPCs. Interfering TUG1 or overexpressing bFGF suppressed osteogenic differentiation of TSPCs. In addition, lncRNA TUG1 interacted with bFGF, and lncRNA TUG1 promoted the ubiquitination of bFGF protein. We also determined that lncRNA TUG1 downregulated bFGF protein expression through promoting the ubiquitination of bFGF. LncRNA TUG1 promoted the osteogenic differentiation of TSPCs through promoting bFGF ubiquitination.

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Financial support

This study was supported by fund of the Zhejiang Provincial Natural Science Foundation (No.Y18H060051), Zhejiang Medical and Health Science and Technology Project (No. 2019RC218), and Wenzhou Municipal Science and Technology Bureau (No. Y20190077).

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Authors and Affiliations

  1. Department of Traumatic Orthopaedics, Shandong Provincial Hospital Affiliated to Shandong University, No.324, Jingwu Road, Jinan, Shandong, 250021, People’s Republic of China

    Yang Yu & Dong-sheng Zhou

  2. Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children’s Hospital, Wenzhou Medical University, Wenzhou, China

    Yang Yu, Yi-jing Zheng, Qi-hao Weng & Si-pin Zhu

  3. Department of Emergency, The Second Affiliated Hospital and Yuying Children’s Hospital, Wenzhou Medical University, Wenzhou, China

    Ying Chen

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  1. Yang Yu
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  2. Ying Chen
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  3. Yi-jing Zheng
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  4. Qi-hao Weng
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  5. Si-pin Zhu
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  6. Dong-sheng Zhou
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Correspondence to Dong-sheng Zhou.

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Editor: Tetsuji Okamoto

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Yu, Y., Chen, Y., Zheng, Yj. et al. LncRNA TUG1 promoted osteogenic differentiation through promoting bFGF ubiquitination. In Vitro Cell.Dev.Biol.-Animal 56, 42–48 (2020). https://doi.org/10.1007/s11626-019-00410-y

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  • DOI: https://doi.org/10.1007/s11626-019-00410-y

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