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A lncRNA promotes myoblast proliferation by up-regulating GH1

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Abstract

Long noncoding RNAs (lncRNAs) are key regulatory factors for gene expression in a variety of biological processes; however, the role of lncRNAs in muscle formation and development is poorly understood, particularly in cattle. Here, we identified a highly expressed lncRNA in muscle, lncYYW, by high-throughput sequencing in bovine longissimus, scapular, intercostal, and gluteus muscles. The expression of lncYYW increased gradually during myoblast differentiation. Overexpression of lncYYW increased the number of cells in the DNA synthesis (S) stage of the cell cycle and upregulated the expression of two well-established myogenic markers, myogenin and myosin heavy chain. A microarray analysis showed that lncYYW positively regulates the expression of growth hormone 1 and its downstream genes, AKT1 and PIK3CD, in bovine myoblasts. This discovery provides a good foundation for further study of the mechanism of action of lncYYW during bovine myoblast development. Taken together, our results reveal a novel lncRNA associated with bovine myoblast proliferation and differentiation. This lncRNA will play a crucial and critical role in future studies of bovine muscle development.

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Acknowledgements

This study was supported by grants from the National Natural Science Foundation of China (31572380) and the National Natural Science Foundation of Tianjin (16JCZDJC33700).

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Authors and Affiliations

  1. College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300384, China

    Yingwei Yue, Congfei Jin, Mingming Chen, Linlin Zhang, Xinfeng Liu, Wenzhi Ma & Hong Guo

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  1. Yingwei Yue
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  2. Congfei Jin
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  3. Mingming Chen
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  4. Linlin Zhang
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  5. Xinfeng Liu
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  6. Wenzhi Ma
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  7. Hong Guo
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Correspondence to Hong Guo.

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Editor: Tetsuji Okamoto

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Yue, Y., Jin, C., Chen, M. et al. A lncRNA promotes myoblast proliferation by up-regulating GH1. In Vitro Cell.Dev.Biol.-Animal 53, 699–705 (2017). https://doi.org/10.1007/s11626-017-0180-z

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  • DOI: https://doi.org/10.1007/s11626-017-0180-z

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