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Americanin B protects cultured human keratinocytes against oxidative stress by exerting antioxidant effects

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Abstract

We evaluated the cytoprotective effects of americanin B, a lignan compound, against hydrogen peroxide (H2O2)-induced cell damage. Americanin B decreased the level of DPPH radicals, superoxide anions, hydroxyl radicals, and intracellular reactive oxygen species. Americanin B also attenuated DNA damage induced by H2O2 treatment, as shown by the inhibition of formation of comet tails, indicative of DNA strand breakage, and prevented the oxidation of protein and peroxidation of lipid, as determined by protein carbonyls and 8-isoprostane. Furthermore, americanin B protected against H2O2-induced apoptotic cell death, as determined by a reduction in the numbers of apoptotic bodies stained with Hoechst 33342. These findings suggest that americanin B protects cells against oxidative damage by exerting antioxidant effects and inhibiting apoptosis.

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Acknowledgments

This research was financially supported by the Ministry of Trade, Industry and Energy (MOTIE) and the Korea Institute for Advancement of Technology (KIAT) through the Promoting Regional Specialized Industry.

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Authors and Affiliations

  1. School of Medicine and Institute for Nuclear Science and Technology, Jeju National University, Jeju, 690-756, Korea

    Jian Zheng, Mei Jing Piao, Ki Cheon Kim, Cheng Wen Yao, Ji Won Cha, Seong Min Kim, Chang Lim Hyun & Jin Won Hyun

  2. Aging Research Center, Korea Institute of Oriental Medicine, Daejeon, 305-811, Korea

    Sungwook Chae

  3. Research Institute of Processing from Jeju Fisher Food, Choung Ryong Fisheries Co., Ltd., Jeju, 697-943, Korea

    Yong Seok Ahn

  4. School of Medicine, Jeju National University, Jeju, 690-756, Korea

    Jin Won Hyun

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  1. Jian Zheng
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Correspondence to Jin Won Hyun.

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Zheng, J., Piao, M.J., Kim, K.C. et al. Americanin B protects cultured human keratinocytes against oxidative stress by exerting antioxidant effects. In Vitro Cell.Dev.Biol.-Animal 50, 766–777 (2014). https://doi.org/10.1007/s11626-014-9759-9

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