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Isolation, characterization, and mesodermic differentiation of stem cells from adipose tissue of camel (Camelus dromedarius)

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Abstract

Adipose-derived stem cells are an attractive alternative as a source of stem cells that can easily be extracted from adipose tissue. Isolation, characterization, and multi-lineage differentiation of adipose-derived stem cells have been described for human and a number of other species. Here we aimed to isolate and characterize camel adipose-derived stromal cell frequency and growth characteristics and assess their adipogenic, osteogenic, and chondrogenic differentiation potential. Samples were obtained from five adult dromedary camels. Fat from abdominal deposits were obtained from each camel and adipose-derived stem cells were isolated by enzymatic digestion as previously reported elsewhere for adipose tissue. Cultures were kept until confluency and subsequently were subjected to differentiation protocols to evaluate adipogenic, osteogenic, and chondrogenic potential. The morphology of resultant camel adipose-derived stem cells appeared to be spindle-shaped fibroblastic morphology, and these cells retained their biological properties during in vitro expansion with no sign of abnormality in karyotype. Under inductive conditions, primary adipose-derived stem cells maintained their lineage differentiation potential into adipogenic, osteogenic, and chondrogenic lineages during subsequent passages. Our observation showed that like human lipoaspirate, camel adipose tissue also contain multi-potent cells and may represent an important stem cell source both for veterinary cell therapy and preclinical studies as well.

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Acknowledgments

This work was supported by a grant from the Stem Cell Technology Research Center, Tehran, Iran. The authors like to thank Miss Lida Langroudi for English editing the manuscript.

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Authors and Affiliations

  1. Stem Cell Biology Department, Stem Cell Technology Research Center, Tehran, Iran

    Abdollah Mohammadi-Sangcheshmeh, Abbas Shafiee, Ehsan Seyedjafari, Peyman Dinarvand, Iman Bagherizadeh & Masoud Soleimani

  2. Department of Animal and Poultry Science, College of Aburaihan, University of Tehran, Pakdasht, Tehran, Iran

    Abdollah Mohammadi-Sangcheshmeh

  3. Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran

    Ehsan Seyedjafari

  4. Department of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran

    Abdolhakim Toghdory

  5. Institute of Animal Science, University of Bonn, Endenicher Allee 15, 53115, Bonn, Germany

    Karl Schellander & Mehmet Ulas Cinar

  6. Department of Hematology, Faculty of Medical Science, Tarbiat Modares University, P.O. Box 14115-111, Tehran, Iran

    Masoud Soleimani

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  1. Abdollah Mohammadi-Sangcheshmeh
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  2. Abbas Shafiee
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Correspondence to Masoud Soleimani.

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Editor: T. Okamoto

Abdollah Mohammadi-Sangcheshmeh and Abbas Shafiee have equally contributed to this publication.

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Mohammadi-Sangcheshmeh, A., Shafiee, A., Seyedjafari, E. et al. Isolation, characterization, and mesodermic differentiation of stem cells from adipose tissue of camel (Camelus dromedarius). In Vitro Cell.Dev.Biol.-Animal 49, 147–154 (2013). https://doi.org/10.1007/s11626-012-9578-9

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  • DOI: https://doi.org/10.1007/s11626-012-9578-9

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