Skip to main content
SpringerLink
Log in

Growth hormone pathway gene expression varies in porcine cumulus–oocyte complexes during in vitro maturation

  • Report
  • Published:
In Vitro Cellular & Developmental Biology - Animal Aims and scope Submit manuscript

Abstract

Growth hormone (GH) plays important roles in oocyte development and facilitates the successful production of competent oocytes in many species both in vivo and in vitro. However, the mechanism of GH action on oocyte maturation is not well known. In this paper, the temporospatial messenger ribonucleic acid expression patterns of GH and several other GH-related factors were quantitatively analyzed in porcine cumulus–oocytes complex throughout in vitro maturation (IVM). GH expression was decreased in oocytes during IVM while absent in cumulus cells. GH receptor, insulin-like growth factor-1 (IGF-1), and IGF-1 receptor expressions were also downregulated in oocytes. In cumulus cells, the expression of IGF-1 decreased significantly while IGF-1 receptor expression remained constant. The transcripts of Janus kinase 2 increased in both oocytes and cumulus cells during IVM. The current precise gene expression information provides further evidence to explain the complex network of GH signaling involved in IVM of porcine oocyte.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1.
Figure 2.

Similar content being viewed by others

References

  • Ali A.; Benkhalifa M.; Miron P. In-vitro maturation of oocytes: biological aspects. Reprod. Biomed. Online 13: 437–446; 2006.

    Article  PubMed  CAS  Google Scholar 

  • Apa R.; Lanzone A.; Miceli F.; Mastrandrea M.; Caruso A.; Mancuso S.; Canipari R. Growth hormone induces in vitro maturation of follicle- and cumulus-enclosed rat oocytes. Mol. Cell. Endocrinol. 106: 207–212; 1994 doi:10.1016/0303-7207(94)90204-6.

    Article  PubMed  CAS  Google Scholar 

  • Bevers M. M.; Izadyar F. Role of growth hormone and growth hormone receptor in oocyte maturation. Mol. Cell. Endocrinol. 197: 173–178; 2002 doi:10.1016/S0303-7207(02)00270-8.

    Article  PubMed  CAS  Google Scholar 

  • Hull K. L.; Harvey S. Growth hormone: roles in female reproduction. J. Endocrinol. 168: 1–23; 2001 doi:10.1677/joe.0.1680001.

    Article  PubMed  CAS  Google Scholar 

  • Izadyar F.; Van Tol H. T.; Colenbrander B.; Bevers M. M. Stimulatory effect of growth hormone on in vitro maturation of bovine oocytes is exerted through cumulus cells and not mediated by IGF-I. Mol. Reprod. Dev. 47: 175–180; 1997 doi:10.1002/(SICI)1098-2795(199706)47:2<175::AID-MRD8>3.0.CO;2-J.

    Article  PubMed  CAS  Google Scholar 

  • Marchal R.; Caillaud M.; Martoriati A.; Gerard N.; Mermillod P.; Goudet G. Effect of growth hormone (GH) on in vitro nuclear and cytoplasmic oocyte maturation, cumulus expansion, hyaluronan synthases, and connexins 32 and 43 expression, and GH receptor messenger RNA expression in equine and porcine species. Biol. Reprod. 69: 1013–1022; 2003 doi:10.1095/biolreprod.103.015602.

    Article  PubMed  CAS  Google Scholar 

  • Petrova I.; Rajmon R.; Sedmikova M.; Kuthanova Z.; Jilek F.; Rozinek J. Improvement of developmental competence of aged porcine oocytes by means of the synergistic effect of insulin-like growth factor-1 (IGF-1) and epidermal growth factor (EGF). Czech J. Anim. Sci. 50: 300–310; 2005.

    CAS  Google Scholar 

  • Rosenfeld R. G.; Belgorosky A.; Camacho-Hubner C.; Savage M. O.; Wit J. M.; Hwa V. Defects in growth hormone receptor signaling. Trends Endocrinol. Metab. 18: 134–141; 2007 doi:10.1016/j.tem.2007.03.004.

    Article  PubMed  CAS  Google Scholar 

  • Singh B.; Armstrong D. T. Insulin-like growth factor-1, a component of serum that enables porcine cumulus cells to expand in response to follicle-stimulating hormone in vitro. Biol. Reprod. 56: 1370–1375; 1997 doi:10.1095/biolreprod56.6.1370.

    Article  PubMed  CAS  Google Scholar 

  • Yoshida Y.; Miyamura M.; Hamano S.; Yoshida M. Expression of growth factor ligand and their receptor mRNAs in bovine ova during in vitro maturation and after fertilization in vitro. J. Vet. Med. Sci. 60: 549–554; 1998 doi:10.1292/jvms.60.549.

    Article  PubMed  CAS  Google Scholar 

  • Zhu G. Y.; Guo B. G.; Pan D. K.; Mu Y. L.; Feng S. T. Expression of bone morphogenetic proteins and receptors in porcine cumulus–oocyte complexes during in vitro maturation. Animal Reprod. Sci. 104: 275–283; 2008 doi:10.1016/j.anireprosci.2007.02.011.

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This research was supported by the Science and Research Start-up Project for the Recruit Talent of Taishan University under grant Y07-2-17.

Author information

Authors and Affiliations

  1. Department of Biology Science and Technology, Taishan University, Taian, 271021, People’s Republic of China

    Guiyu Zhu, Shujuan Liu, Honghua Yang & Jinlian Li

  2. College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian, 271018, People’s Republic of China

    Yunliang Jiang

Authors
  1. Guiyu Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shujuan Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yunliang Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Honghua Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jinlian Li
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Guiyu Zhu.

Additional information

Editor: J. Denry Sato

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhu, G., Liu, S., Jiang, Y. et al. Growth hormone pathway gene expression varies in porcine cumulus–oocyte complexes during in vitro maturation. In Vitro Cell.Dev.Biol.-Animal 44, 305–308 (2008). https://doi.org/10.1007/s11626-008-9130-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11626-008-9130-0

Keywords

Search

Navigation