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Silencing Filamin A Inhibits the Invasion and Migration of Breast Cancer Cells by Up-regulating 14-3-3σ

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Summary

Filamin A and 14-3-3-σ are closely associated with the development of breast cancer. However, the exact relationship between them is still unknown. The present study aimed to examine the interaction of filamin A with 14-3-3-σ in the invasion and migration of breast cancer. RNA interference technology was employed to silence filamin A in MDA-MB-231 cells. Real-time PCR and Western blotting were used to detect the expression of filamin A and 14-3-3-σ at mRNA and protein levels, respectively. Double immunofluorescence was applied to show their colocalization morphologically. Wound healing assay and Trans-well assay were used to testify the migration and invasion of MDA-MB-231 cells in filamin A-silenced cells. The results showed that silencing filamin A significantly increased the mRNA and protein levels of 14-3-3σ. In addition, double immunofluorescence displayed that filamin A and 14-3-3σ were predominantly colocalized in the cytoplasm of MDA-MB-231 cells. Silencing filamin A led to the enhanced fluorescence of 14-3-3σ. Furthermore, cell functional experiments showed that silencing filamin A inhibited the migration and invasion of MDA-MB-231 cells in vitro. In conclusion, silencing filamin A may inhibit the invasion and migration of breast cancer cells by upregulating 14-3-3σ.

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Acknowledgments

We are deeply indebted to Dr. Chun-wei SHI for her technique assistance.

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Authors and Affiliations

  1. Institute of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Zhi-min Ji, Li-li Yang, Juan Ni, San-peng Xu, Cheng Yang, Pei Duan, Li-ping Lou & Qiu-rong Ruan

  2. Puren Hospital Affiliated to Wuhan University of Science and Technology, Wuhan, 430080, China

    Zhi-min Ji

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  1. Zhi-min Ji
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  2. Li-li Yang
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  3. Juan Ni
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Correspondence to Qiu-rong Ruan.

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Ji, Zm., Yang, Ll., Ni, J. et al. Silencing Filamin A Inhibits the Invasion and Migration of Breast Cancer Cells by Up-regulating 14-3-3σ. CURR MED SCI 38, 461–466 (2018). https://doi.org/10.1007/s11596-018-1901-6

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  • DOI: https://doi.org/10.1007/s11596-018-1901-6

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