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IFN-γ secretion in gut of Ob/Ob mice after vertical sleeve gastrectomy and its function in weight loss mechanism

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Summary

Vertical sleeve gastrectomy (VSG) is becoming more and more popular among the world. Despite its dramatic efficacy, however, the mechanism of VSG remains largely undetermined. This study aimed to test interferon (IFN)-γ secretion n of mesenteric lymph nodes in obese mice (ob/ob mice), a model of VSG, and its relationship with farnesoid X receptor (FXR) expression in the liver and small intestine, and to investigate the weight loss mechanism of VSG. The wild type (WT) mice and ob/ob mice were divided into four groups: A (WT+Sham), B (WT+VSG), C (ob/ob+Sham), and D (ob/ob+VSG). Body weight values were monitored. The IFN-γ expression in mesenteric lymph nodes of ob/ob mice pre- and post-operation was detected by flow cytometry (FCM). The FXR expression in the liver and small intestine was detected by Western blotting. The mouse AML-12 liver cells were stimulated with IFN-γ at different concentrations in vitro. The changes of FXR expression were also examined. The results showed that the body weight of ob/ob mice was significantly declined from (40.6±2.7) g to (27.5±3.8) g on the 30th day after VSG (P<0.05). At the same time, VSG induced a higher level secretion of IFN-γ in mesenteric lymph nodes of ob/ob mice than that pre-operation (P<0.05). The FXR expression levels in the liver and small intestine after VSG were respectively 0.97±0.07 and 0.84±0.07 fold of GAPDH, which were significantly higher than pre-operative levels of 0.50±0.06 and 0.48±0.06 respectively (P<0.05). After the stimulation of AML-12 liver cells in vitro by different concentrations of IFN-γ (0, 10, 25, 50, 100, and 200 ng/mL), the relative FXR expression levels were 0.22±0.04, 0.31±0.04, 0.39±0.05, 0.38±0.05, 0.56±0.06, and 0.35±0.05, respectively, suggesting IFN-γ could distinctly promote the FXR expression in a dose-dependent manner in comparison to those cells without IFN-γ stimulation (P<0.05). It was concluded that VSG induces a weight loss in ob/ob mice by increasing IFN-γ secretion of mesenteric lymph nodes, which then increases the FXR expression of the liver and small intestine.

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Authors and Affiliations

  1. Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China

    Jin-peng Du  (杜锦鹏), Geng Wang  (汪 赓), Chao-jie Hu  (胡超杰), Qing-bo Wang  (王清波), Hui-qing Li  (黎慧清), Wen-fang Xia  (夏文芳), Xiao-ming Shuai  (帅晓明), Kai-xiong Tao  (陶凯雄), Guo-bin Wang  (王国斌) & Ze-feng Xia  (夏泽锋)

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  1. Jin-peng Du  (杜锦鹏)
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  2. Geng Wang  (汪 赓)
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  3. Chao-jie Hu  (胡超杰)
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  4. Qing-bo Wang  (王清波)
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  5. Hui-qing Li  (黎慧清)
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  6. Wen-fang Xia  (夏文芳)
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  7. Xiao-ming Shuai  (帅晓明)
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  8. Kai-xiong Tao  (陶凯雄)
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Correspondence to Ze-feng Xia  (夏泽锋).

Additional information

This study was supported by grants from the National Natural Science Foundation of China (No. 81200276), the Hubei Provincial Natural Science Foundation of China (No. 2015CFB710), the Health and Family Planning Youth Project Foundation of Hubei Province, China (No. WJ2015Q001), the Research Fund of Union Hospital of Huazhong University of Science and Technology, China (No. 000003396), and the Research Fund of Public Welfare in Health Industry, 2014, Health and Family Planning Commission of China (No. 201402015), 2014, Health Ministry of China.

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Du, Jp., Wang, G., Hu, Cj. et al. IFN-γ secretion in gut of Ob/Ob mice after vertical sleeve gastrectomy and its function in weight loss mechanism. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 36, 377–382 (2016). https://doi.org/10.1007/s11596-016-1595-6

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  • DOI: https://doi.org/10.1007/s11596-016-1595-6

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