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Local immune regulatory effects of Bangdeyun on the endometrium of mice with embryo implantation dysfunction during the implantation time

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Summary

This study examined the effects of Bangdeyun on the expressions of nuclear factor-kappaB (NF-κB), interferon-gamma (IFN-γ) and interleukin-10 (IL-10) in the endometrium of mice with embryo implantation dysfunction (EID) during the implantation time (namely on pregnancy day 5, 6, 7 and 8) and explored the local immune regulatory effects of Bangdeyun. The gestational mice were randomly divided into normal group, model group and Bangdeyun-treated group. EID models of mice were established by using indomethacin. The endometrial expression of NF-κB was detected by immunohistochemistry and Western blotting. IFN-γ and IL-10 were measured by enzyme-linked immunosorbent assay (ELISA). The results showed that in the normal group, NF-κB and IFN-γ were weakly expressed and IL-10 was strongly expressed in the endometrium during the whole implantation period. In the model group, the expressions of NF-κB and IFN-γ were increased on pregnancy day 5, 6 and 7, and IL-10 expression decreased during the whole implantation time when compared with those in the normal group (P<0.01 for all). In the Bangdeyun-treated group, little amount of NF-κB and IFN-γ was expressed and IL-10 expression was strong, much the way they were expressed in the normal group (P>0.05). The expressions of NF-κB and IFN-γ were much lower in the Bangdeyun-treated group than those in the model group on pregnancy day 5, 6 and 7 (P<0.01 for all), while the expression of IL-10 was much higher than in the model group during the whole implantation time (P<0.01). It was suggested Bangderun may favor a shift from Th1- to Th2-type immune response, therefore inhibiting the maternal immune rejection, inducing the immune tolerance and improving the fetal implantation.

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Authors and Affiliations

  1. Hubei Key Laboratory of Natural Pharmacochemistry and Resources Evaluation, Department of Traditional Chinese Herbs, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Yunxia Wu  (吴云霞)

  2. Institute of Integrated Traditional Chinese Medicine and Western Medicine, Huazhong University of Science and Technology, Wuhan, 430030, China

    Cuihong Zheng  (郑翠红)

  3. Center of Reproductive Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Linli Hu  (胡琳丽)

  4. Department of Emergency, Hubei General Hospital of Chinese People’s Armed Police Forces, Wuhan, 430060, China

    Jing Li  (李靖)

Authors
  1. Yunxia Wu  (吴云霞)
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  2. Cuihong Zheng  (郑翠红)
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  3. Linli Hu  (胡琳丽)
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  4. Jing Li  (李靖)
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Additional information

This project was supported by a grant from National Natural Science Foundation of China (No. 30801538).

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Wu, Y., Zheng, C., Hu, L. et al. Local immune regulatory effects of Bangdeyun on the endometrium of mice with embryo implantation dysfunction during the implantation time. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 29, 372–376 (2009). https://doi.org/10.1007/s11596-009-0322-y

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  • DOI: https://doi.org/10.1007/s11596-009-0322-y

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