Summary
To investigate the distribution of the genes of two major metallo-β-lactamases (MBL; i.e., IMP and VIM) and class 1 integrons (intI) in the clinical imipenem-resistant Pseudomonas aeruginosa, a total of 65 isolates, from a university hospital in Sichuan between December 2004 and April 2005 were screened for MBL genes by PCR using primers specific for bla IMP-1 , bla VIM and bla VIM-2 genes. The MBL-positive isolates were further assessed for class 1 integrons by PCR using specific primers. The nucleotide sequences of several PCR products were also determined. The results revealed that the bla VIM gene was found in 81.5% (53/65) of all isolates, bla VIM-2 gene was found in only 1 isolate and the intI gene was observed in 45.3% (24/53) of bla VIM -positive isolates. One isolate carried simultaneously both bla IMP-1 and intI genes, and to the best of our knowledge this is the first report of such isolate in southwest China. These observations highlight that the genes for VIM β-lactamase and class 1 integrons were predominantly present among the imipenem-resistant P. aeruginosa tested, confirming the current widespread threat of imipenem-resistant, integron-borne P. aeruginosa.
Article PDF
Similar content being viewed by others
References
Shiri N V, Ronen B A, Yehuda C. Update on Pseudomonas aeruginosa and Acinetobacter baumannii infections in the healthcare setting. Curr Opin Infect Dis, 2005,18(4):306–313
Livermore D M, Woodford N. Carbapenemases: a problem in waiting? Curr Opin Microbiol, 2000,3(5):489–495
Queenan A M, Bush K. Carbapenemases: the versatile β-lactamases. Clin Microbiol Rev, 2007,20(3):440–458
Boucher Y, Labbate M, Koenig J E et al. Integrons: mobilizable platforms that promote genetic diversity in bacteria. Trend Microbiol, 2007,15(7):301–309
Yang Q, Wei Q Z, Yu Y S et al. Study on the VIM-2 metallo-beta-lactamase-producing Pseudomonas aeruginosa strains in intensive care unit. Chin J Lab Med (Chinese), 2004,27(10):678–682
Zhao Z W, Zhong W N, Ma W. Clinical analysis on an outbreak of ventilator-associated pneumonia caused by metallo-β-lactamase-producing Pseudomonas aeruginosa in respiratory intensive care unit. Chin J Resp Critical Care Med (Chinese), 2006,5(3):168–171
Yang W Q, Shi L, Yin X L et al. Distribution and identification of class 1 integrons and integrated gene cassettes in clinical Pseudomonas aeruginosa in different geographic regions. Chin J Antibiot (Chinese), 2006,31(1):15–18
Senda K, Arakawa Y, Ichiyama S et al. PCR detection of metallo-β-lactamase gene (bla IMP) in gram-negative rods resistant to broad-spectrum β-lactams. J Clin Microbiol, 1996,34(12):2909–2913
Watanabe M, Iyobe S, Inoue M et al. Transferable imipenem resistance in Pseudomonas aeruginosa. Antimicrob. Agents Chemother, 1991,35(1):147–151
Cornaqlia G, Mazzariol A, Lauretti L et al. Hospital outbreak of carbapenem-resistant Pseudomonas aeruginosa producing VIM-1, a novel transferable metallo-β-lactamase. Clin Infect Dis, 2000,31(5):1119–1125
Zhang Y B, Song s D, Qi W et al. Detection of metallo-β-lactamase genes (Imp-1, Vim-1 and Vim-2) by PCR in clinical isolates of imipenem-resistant Pseudomonas Aeruginosa. Tianjing Med J, 2003,30(3):131–133
Chen R, Peng X M, Zhu J X et al. VIM-2 metallo-β-lactamase producing Pseudomonas aeruginosa in brun wards. Clin J Nosocomiol, 2006,16(7):734–737
Xu Y H, Shen J L, Yang B X et al. Detection of metallo-beta-lactamase as well as loss of out membrane protein from Pseudomonas aeruginosa. Clin J Nosocomiol, 2005,15(10):1085–1089
Li X Z. Quinolone resistance in bacteria: emphasis on plasmid-mediated mechanisms. Int J Antimicrob Agents, 2005,25(6):453–463
Li X Z, Nikaido H. Efflux-mediated drug resistance in bacteria. Drugs, 2004,64(2):159–204
Maniati M, Ikonomidis A, Mantzana P et al. A highly carbapenem-resistant Pseudomonas aeruginosa isolate with a novel bla VIM-4/bla PIb integron overexpresses two efflux pumps and lacks OprD. J Antimicrob Chemother, 2007,60(1):132–135
Author information
Authors and Affiliations
Corresponding author
Additional information
This project was supported by a grant from National Natural Science Foundation of China (No. 30370079).
Rights and permissions
About this article
Cite this article
Cheng, X., Wang, P., Wang, Y. et al. Identification and distribution of the clinical isolates of imipenem-resistant Pseudomonas aeruginosa carrying metallo-β-lactamase and/or class 1 integron genes. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 28, 235–238 (2008). https://doi.org/10.1007/s11596-008-0301-8
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11596-008-0301-8