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Analysis of hupehenine in the total alkaloids from Fritillaria hupehensis by HPLC-ELSD

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Summary

A reverse-phase high pressure liquid chromatography (HPLC) method with evaporative light scattering detection (ELSD) has been developed for the quantitative analysis of hupehenine in the total alkaloids from Fritillaria hupehensis. Samples were analyzed on a reverse-phase column (Hypersil C-18) with a mobile phase of methanol:water:chloroform: triethylamine (85:15:1:0.6). The ELSD was set at the drift tube temperature of 68.3°C and gas flow rate of 1.8 L/min. Hupehenine’s retention time was 13.7 min with an asymmetry factor of 1.2. The validity of the method has been verified with linearity, limit of detection, accuracy and precision. The logarithmic linear curve was obtained from 8.936 to 134.04 μg/mL (r=0.9993). The detection limit (S/N>3) of hupehenine was 1.79 μg/mL on the column. Intra-day RSD was 1.42% and inter-day RSD was 2.26% (3 days within a week). The average recovery of hupehenine was 101.50%, and RSD was 1.62%.

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References

  1. State Pharmacopoeia Committee of the People’s Republic of China. Pharmacopoeia of the People’s Republic of China (Chinese). 2000ED. Beijing: Chemical Industry Press, 2000, 286–287.

    Google Scholar 

  2. Stolyhuo A, Colin H, Guiochon G. Use of light scattering as a detector principle in liquid chromatography. J Chromatogr, 1983,265:1–18

    Article  Google Scholar 

  3. Zhao Y Q. Analytic Method of Chinese Traditional Herb Drugs and Preparations (Chinese). Shenyang: Liaoning Publishing House of Sciences and Technology, 1995,93–95

    Google Scholar 

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Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Peng Zhang  (张 鹏), Juan Li  (李 娟), Guoxin Zhang  (张国欣), Huifang Pi  (皮慧芳), Yonghui Zhang  (张勇慧), Hanli Ruan  (阮汉利) & Jizhou Wu  (吴继洲)

Authors
  1. Peng Zhang  (张 鹏)
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  2. Juan Li  (李 娟)
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  3. Guoxin Zhang  (张国欣)
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  4. Huifang Pi  (皮慧芳)
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  5. Yonghui Zhang  (张勇慧)
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  6. Hanli Ruan  (阮汉利)
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  7. Jizhou Wu  (吴继洲)
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Additional information

ZHANG Peng, male, born in 1979, M.D., Ph.D.

This project was supported by a grant from the State Administration of Traditional Chinese Medicine of China (No. D2X004A).

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Zhang, P., Li, J., Zhang, G. et al. Analysis of hupehenine in the total alkaloids from Fritillaria hupehensis by HPLC-ELSD. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 28, 118–120 (2008). https://doi.org/10.1007/s11596-008-0130-9

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  • DOI: https://doi.org/10.1007/s11596-008-0130-9

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