Summary
In order to investigate the protective effects of the overexpression of bcl-xl gene on local cerebral infarction in the transgenic mice subject to permanent occlusion of middle cerebral artery, the models of bcl-xl transgenic mice were established and subjected to cerebral infarction by intraluminal occlusion of the middle cerebral artery. The infarct volume and the neurological scores were observed and comparison between the wild type mice and the transgenic mice was made. It was found that the infarct volume and the neurological scores in the transgenic mice were significantly decreased as compared with those in the wild type mice. It was suggested that the overexpression of bcl-xl gene in transgenic mice could reduce the infarct volume and improve the neurological function of the mice.
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References
Gonzalez-Garcia M, Garcia I, Ding L et al. Bcl-x is expressed in embryonic and postnatal neural tissues and functions to prevent neuronal cell death. Proc Natl Acad Sci USA, 1992,92:4304–4308
Parsadanian A S, Cheng Y, Keller-Peck C R et al. Bcl-xL is an antiapoptotic regulator for postnatal CNS neurons. J Neurosci, 1998,18(3):1009–1019
Xiao W W, Wang F R, Zhang S M et al. The establishment of transgenic mice with microinjection. J China Neurosci (Chinese), 2002,18(3):630–634
Xiao W W, Wang F R, Zhang S M et al. The establishment and detection of transgenic mice with microinjection. J Huazhong Univ Sci Technol (Med Ed) (Chinese), 2003,32(2):180–181.
Wang F R, Yuan H, Xiao W W et al. Observation on reproduction and stable transmissibility of foreign gene in human Bcl-xl transgenic mice. J Huazhong Univ Sci Technol (Med Ed) (Chinese), 2002,31(6):615–617.
Wang F R, Xiao W W, Zhang S M et al. Using a line bolt in the internal carotid artery to establish the model of partial cerebral ischemia and reperfusion of mice. J Stroke Neural Dis (Chinese), 2003,10(2):112–114.
Connolly Jr E S, Winfree C J, Stern D M et al. Procedural and strain-related variables significantly affect outcome in a murine model of focal cerebral ischemia. Neurosurgery, 1996,38(3):523–532
Hata R, Mies G, Wiessner C et al. A reproducible model of middle cerebral artery occlusion in mice: hemodynamic, biochemical and magnetic resonance imaging. J Cereb Blood Flow Metab, 1998,18(4):367–375
Kim Y C, Shim J W, Oh Y J et al. Co-transfection with cDNA encoding the Bcl family of anti-apoptotic proteins improves the efficiency of transfection in primary fetal neural stem cells. J Neurosci Methods, 2002,117(2): 153–158
Wiessner C, Allegrini P R, Rupalla K et al. Neuron-specific transgene expression of Bcl-xl but not Bcl-2 genes reduced lesion size after permanent middle cerebral artery occlusion in mice. Neurosci Lett, 1999,268(3): 119–122
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Furong WANG, female, born in 1975, Associate Professor, M.D., Ph.D.
This project was supported by a grant from National Natural Sciences Foundation of China (No. 30500167).
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Wang, F., Jiang, Y., Zhang, S. et al. Protective effects of overexpression of bcl-xl gene on local cerebral infarction in transgenic mice undergoing permanent occlusion of middle cerebral artery. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 28, 56–59 (2008). https://doi.org/10.1007/s11596-008-0114-9
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DOI: https://doi.org/10.1007/s11596-008-0114-9