Summary
In order to explore the method to prepare hypoxia UW solution and the stability and preservation of hypoxia UW solution, UW solution was purged by argon or air for 15 min or 60 at a flow rate of 0.8 or 2 L/min, and the oxygen partial pressure of UW solution was detected. The hypoxia UW solution was exposed to the air or sealed up to preserve by using different methods, and the changes of oxygen partial pressure was tested. The results showed that oxygen partial presure of 50 mL UW solution, purged by argon for 15 min at a flow rate of 2 L/min, was declined from 242±6 mmHg to 83±10 mmHg. After exposure to the air, oxygen partial pressure of hypoxia UW solution was gradually increased to 160±7 mmHg at 48 h. After sealed up by the centrifuge tube and plastic bad filled with argon, oxygen partial pressure of hypoxia UW solution was stable, about 88±13 mmHg at 72 h. It was concluded that oxygen of UW solution could be purged by argon efficiently. Sealed up by the centrifuge tube and plastic bag filled with argon, oxygen partial pressure of UW solution could be stabilized.
Similar content being viewed by others
References
Lee P J, Choi A M. Pathways of cell signaling in hyperoxia. Free Radic Biol Med, 2003,35:341–350
Southard J H. Viability assays in organ preservation. Cryobiology, 1989,26:232–238
Shen Y L, Wang B Z. Dynamic mechanism of removal of ammonia in extravasate by purge method. Wuran Fangzhi Jishu (Chinese), 1999,12:67–70
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Wan, C., Wang, C., Liu, T. et al. Preparation and preservation of hypoxia UW solution. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 27, 554–556 (2007). https://doi.org/10.1007/s11596-007-0520-4
Received:
Issue Date:
DOI: https://doi.org/10.1007/s11596-007-0520-4