Abstract
The unique mode of nutrition by carnivorous plants makes the facilitation of nutrient acquisition by mycorrhizal fungi seem unlikely. However, previously we have reported that the carnivorous plant Drosera rotundifolia can host at least eight species of fungal root endophyte. Although the function of these fungi remains unknown, their ubiquitous presence suggests that they may give plants a competitive advantage in both stressful and nutrient poor environments. The aim of this study was to examine the species of fungal endophyte colonising the roots of Pinguicula vulgaris and to compare them with the species isolated from D. rotundifolia found growing in the same location. Trichoderma spp. were isolated from every plant, whilst four other species were isolated once from single plants (Neonectria sp., Leohumicola spp., Cladosporium macrocarpum and Volutella ciliata). Although Trichoderma were isolated from both carnivorous plants, all of the other fungal endophytes were found colonising either P. vulgaris or D. rotundifolia. This observation may indicate a degree of host-specificity for these fungal species, despite the two host plants having very similar ecological strategies for living in a very nutrient poor and abiotically stressful environment.
References
Brundrett MC (2009) Mycorrhizal associations and other means of nutrition of vascular plants: understanding the global diversity of host plants by resolving conflicting information and developing reliable means of diagnosis. Plant Soil 320:37–77
Chambers SM, Curlevski NJA, Cairney JWG (2008) Ericoid mycorrhizal fungi are common root inhabitants of non-Ericaceae plants in a south-eastern Australian sclerophyll forest. FEMS Microbiol Ecol 65:263–270
Ellison AM, Gotelli NJ (2009) Energetics and the evolution of carnivorous plants - Darwin's ‘most wonderful plants in the world’. J Exp Bot 60:19–42
Fuchs B, Haselwandter K (2004) Red list plants: colonization by arbuscular mycorrhizal fungi and dark septate endophytes. Mycorrhiza 14:277–281
Gardes M, Bruns TD (1993) ITS primers with enhanced specificity for Basidiomycetes – application to the identification of mycorrhizae and rusts. Mol Ecol 2:113–118
Hall TA (1999) BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
Kernaghan G, Patriquin G, 2011. Host associations between fungal root endophytes and boreal trees. Microb Ecol. DOI 10.1007/s00248-011-9851-6 in press.
Kopchinskiy A, Komon M, Kubicek CP, Druzhinina IS (2005) TrichoBLAST, a multilocus database for Trichoderma and Hypocrea identifications. Mycol Res 109:658–660
Mandyam K, Jumpponen A (2005) Seeking the elusive function of the root-colonising dark septate endophytic fungi. Stud Mycol 53:173–189
Quilliam RS, Jones DL (2010) Fungal root endophytes of the carnivorous plant Drosera rotundifolia. Mycorrhiza 20:341–348
Rodriguez RJ, Henson J, Van Volkenburgh E, Hoy M, Wright L, Beckwith F, Kim YO, Redman RS (2008) Stress tolerance in plants via habitat-adapted symbiosis. ISME J 2:404–416
Thompson JD, Higgins DG, Gibson TJ (1994) Clustal-W – improving the sensitivity of progressive multiple sequence alignment through sequence-weighting position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680
Weiß M, Sýkorová Z, Garnica S, Riess K, Martos F, Krause C, Oberwinkler F, Bauer R, Redecker D (2011) Sebacinales everywhere: previously overlooked ubiquitous fungal endophytes. PLoS One 6:e16793
White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Shinsky JJ, White TJ (eds) PCR Protocols: a guide to methods and applications. Academic Press, San Diego, CA, USA, pp 315–322
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This project was funded by a Botanical Society of the British Isles (BSBI) research grant awarded to RSQ.
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Quilliam, R.S., Jones, D.L. Evidence for host-specificity of culturable fungal root endophytes from the carnivorous plant Pinguicula vulgaris (Common Butterwort). Mycol Progress 11, 583–585 (2012). https://doi.org/10.1007/s11557-011-0795-5
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DOI: https://doi.org/10.1007/s11557-011-0795-5