Abstract
The company Appacale started applying molecular markers in 1998 with the implementation of RYSC3 marker of resistance to Potato virus Y (PVY). Since then, five more molecular markers have been implemented, and now it is possible to select for PVY, Globodera rostochiensis and Globodera pallida resistance with this technology. The markers used have allowed the selection of breeding clones with resistance to one or more pathogens that are currently part of the breeding programme, new cultivars or even being used as resistant parents. The results obtained are presented, as well as the advantages and setbacks found in applying Molecular-Assisted Selection from the point of view of a practical breeding programme developed in a small company.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barone A (2004) Molecular marker assisted selection for potato breeding. Am J Potato Res 81:111–117
Barone A, Ritter E, Schachtschabel U, Debenr T, Salamini F, Gebhardt C (1990) Localization by restriction fragment length polymorphism mapping in potato of a major dominant gene conferring resistance to the potato cyst nematode Globodera rostochiensis. Mol Gen Genet 224:177–182
Bryan GJ, McLean K, Bradshaw JE, De Jong WS, Phillips M, Castelli L, Waugh R (2002) Mapping QTLs for resistance to the cyst nematode Globodera pallida derived from the wild potato species Solanum vernei. Theor Appl Genet 105:68–77
Collard BCY, Mackill DJ (2008) Marker-assisted selection: an approach for precision plant breeding in the twenty-first century. Phil Trans R Soc B 363:557–572
Edwards K, Johnstone C, Thompson C (1991) A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucleic Acids Res 19:1349
Gebhardt C, Valkonen JPT (2001) Organization of genes controlling disease resistance in potato genome. Annu Rev Phytopathol 39:79–102
Gebhardt C, Mugniery D, Ritter E, Salamini F, Bonnel E (1993) Identification of RFLP markers closely linked to the H1 gene conferring resistance to Globodera rostochiensis in potato. Theor Appl Genet 85:541–544
Gebhardt C, Bellin D, Henselwski H, Lehmann W, Schwarzfisher J, Valkonen JPT (2006) Marker-assisted combination of major genes for pathogen resistance in potato. Theor Appl Genet 112:1458–1464
Hämälainen JH, Watanabe KN, Valkonen JPT, Arihara A, Plaisted RL, Pehu E, Miller L, Slack SA (1997) Mapping and marker-assisted selection for a gene for extreme resistance to potato virus Y. Theor Appl Genet 94:192–197
Kasai K, Morikawa Y, Sorri VA, Valkonen JPT, Gebbhardt C, Watanabe KN (2000) Development of SCAR markers to the PVY resistance gene Ryadg based on a common feature of plant disease resistance genes. Genome 43:1–8
Lopez-Vizcon C, Ortega F (2011) Molecular marker assisted selection in a small breeding company. In: Abstracts of the 18th EAPR meeting, Oulu, Finland, p. 44
Muñoz FJ, Plaisted RL, Thruston HD (1975) Resistance to potato virus Y in Solanum tuberosum spp. andigena. Am Potato J 52:107–115
Ottoman RJ, Hane DC, Brown CR, Yilma S, James SR, Mosley AR, Crosslin JM, Vales MI (2009) Validation and implementation of marker-assisted selection (MAS) for PVY resistance (Ry adg gene) in a tetraploid potato breeding program. Am J Pot Res 86:304–314
Paal J, Henselewsk H, Muth J, Meksem K, Menéndez CM, Salamini F, Ballvora A, Gebhardt C (2004) Molecular cloning of the potato Gro1-4 gene conferring resistance to pathotype Ro1 of the root cyst nematode Globodera rostochiensis, based on a candidate gene approach. Plant J 38:285–297
Pineda O, Bonierbale MW, Plaisted RL, Brodie BB, Tanksley SD (1993) Identification of RFLP markers linked to the H1 gene conferring resistance to the potato cyst nematode Globodera rostochiensis. Genome 36:152
Ross H (1986) Potato breeding–problems and perspectives. Advances in plant breeding. Supplement 13 to Journal of Plant Breeding, Paul Parey, Berlin, Hamburg, pp 75–82
Ruiz de Arcaute R, Isla S, Carrasco A (2002) New strategies on breeding for PVY resistance. In: Abstracts of the15th EAPR Meeting, Potato today and tomorrow, Hamburg, Vortr Pflanzenzüchtung, p 16
Sattarzadeh A, Achenbach U, Lübeck J, Strahwald J, Tacke E, Hofferbert H-R, Rothsteyn T, Gebhardt C (2006) Single nucleotide polymorphism (SNP) genotyping as basis for developing a PCR-based marker highly diagnostic for potato varieties with high resistance to Globodera pallida pathotype Pa2/3. Mol Breeding 18:301–318
Simko I, Jansky S, Stephenson S, Spooner D (2007) Genetics of resistance to pests and diseases. In: Vreugdenhil D et al. (eds) Potato biology and biotechnology advantages and perspectives. Elsevier, Oxford, pp 117–155
Song YS, Hepting L, Schweizer G, Hartl L, Wenzel G, Schwarzfisher A (2005) Mapping of extreme resistance to PVY (Ry sto ) on chromosome XII using anther-culture-derived primary dihaploid potato lines. Theor Appl Genet 111:879–887
Young ND (1999) A cautiously optimistic vision for marker-assisted breeding. Mol Breed 5:505–510
Zar JH (1974) Biostatistical analysis. Prentice-Hall Inc, Englewood Cliffs
Acknowledgements
We would like to thank Dr. W.S. De Jong who kindly provided us the protocol of TG689 marker and Dr. Ana Carrasco who started to apply the molecular markers in Appacale.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Ortega, F., Lopez-Vizcon, C. Application of Molecular Marker-Assisted Selection (MAS) for Disease Resistance in a Practical Potato Breeding Programme. Potato Res. 55, 1–13 (2012). https://doi.org/10.1007/s11540-011-9202-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11540-011-9202-5