Skip to main content

Advertisement

SpringerLink
Log in

Cryotherapy of Potato Shoot Tips for Efficient Elimination of Potato Leafroll Virus (PLRV) and Potato Virus Y (PVY)

  • Published:
Potato Research Aims and scope Submit manuscript

Abstract

Viral diseases constitute a major constraint to high yield and high quality production of potato. Potato leafroll virus (PLRV) and Potato virus Y (PVY) are among the most damaging potato viruses and are prevalent in most potato growing areas. In the present study, attempts were made to eliminate PLRV and PVY by three cryogenic protocols, i.e., encapsulation-dehydration, encapsulation-vitrification and droplet. Results showed that both PLRV and PVY could be efficiently eliminated by cryogenic treatments with 83–86% and 91–95% of frequencies of virus-free plantlets obtained for the former and latter, respectively. Frequencies of virus-free plantlets produced by cryogenic treatments were higher than those by meristem culture (56% for PLRV and 62% for PVY) and thermotherapy (50% for PLRV and 65% for PVY), and similar to those by thermotherapy followed by meristem culture (90% for PLRV and 93% for PVY). Survival (75–85%) and regrowth (83–89%) from cryo-treated shoot tips were higher than those from meristem culture (50–55%) and thermotherapy followed by meristem culture (40–50%), but similar to those from thermotherapy (80–87%). The morphology of the plantlets regenerated from cryo-treated shoot tips was similar to that of non-treated plantlets. Thus, cryotherapy would provide an alternative method for efficient elimination of potato viruses, and can be simultaneously used for long-term storage of potato germplasm and for production of virus-free plants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

BSV:

Banana streak virus

BAP:

Benzylaminopurine

CMV:

Cucumber mosaic virus

DMSO:

Dimethylsulfoxide

GA3 :

Gibberellic acid 3`

GVA:

Grapevine virus A

LSD:

Least significant difference

LN:

Liquid nitrogen

MS:

Murashige and Skoog medium

PVS2:

Plant vitrification solution 2

PPV:

Plum Pox Potyvirus

PLRV:

Potato leafroll virus

PVY:

Potato virus Y

RAPD:

Random amplified polymorphic DNA

References

  • Benson EE, Wilkinson M, Todd A, Ekuere U, Lyon J (1996) Developmental competence and ploidy stability in plants regenerated from cryopreserved potato shoot-tips. Cryo-lett 17:119–128

    Google Scholar 

  • Bouafia S, Jelti N, Lairy G, Blanc A, Bonnel E, Dereuddre J (1996) Cryopreservation of potato shoot tips by encapsulation-dehydration. Potato Res 39:69–78

    Article  Google Scholar 

  • Brison M, Boucaud M-T, Pierronnet A, Dosba F (1997) Effect of cryopreservation on the sanitary state of a cv. Prunus rootstock experimentally contaminated with Plum Pox Potyvirus. Plant Sci 123:189–196

    Article  CAS  Google Scholar 

  • Brunt A (2001) Potyviruses. In: Loebenstein G, Berger Ph H, Brunt A, Lawson RH (eds) Virus and virus-like diseases of potatoes and production of seed-potatoes. Kluwer, Dordrecht, pp 77–85

    Google Scholar 

  • Clark MF, Adams AN (1977) Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J Gen Virol 34:475–483

    Article  PubMed  CAS  Google Scholar 

  • Engelmann F (1997) In vitro conservation methods. In: Callow JA, Ford-Lloyd BV, Newbury HJ (eds) Biotechnology and plant genetic resources. CAB International, Oxford, pp 119–161

    Google Scholar 

  • Faccioli G (2001) Control of potato viruses using meirstem culture and stem-cutting cultures, thermotherapy and chemotherapy. In: Loebenstein G, Berger Ph H, Brunt A, Lawson RH (eds) Virus and virus-like diseases of potatoes and production of seed-potatoes. Kluwer, Dordrecht, pp 365–390

    Google Scholar 

  • Faccioli G, Colombarini A (1996) Correlation of potato virus S and virus M contents of potato meristem tips with the percentage of virus-free plantlets produced in vitro. Potato Res 39:129–140

    Article  CAS  Google Scholar 

  • Faccioli G, Marani F (1998) Virus elimination by meristem tip culture and tip micrografting. In: Hadidi A, Khetarpal RK, Koganzawa H (eds) Plant virus disease control. APS, St. Paul, MN, pp 346–380

    Google Scholar 

  • Faccioli G, Rubies-Autonell C (1982) PVX and PVY distribution in potato meristem tips and their eradication by the use of thermotherapy and meristem-tip culture. Phytopathol Z 103:66–76

    Google Scholar 

  • Faccioli G, Rubies-Autonell C, Resca R (1988) Potato leafroll virus distribution in potato meristem tips and production of virus-free plants. Potato Res 31:511–520

    Article  Google Scholar 

  • Griffiths HM, Slack SA (1990) Effect of chemical and heat therapy on virus concentrations in in vitro potato plantlets. Can J Bot 68:1515–1521

    CAS  Google Scholar 

  • Hamm PB, Hane DC (1999) Effects of seedborne potato leafroll virus on Russet Norkotah potato. Plant Dis 83:1122–1124

    Article  Google Scholar 

  • Hane DC, Hamm PB (1999) Effects of seedborne potato virus Y infection in two potato cultivars expressing mild disease symptoms. Plant Dis 83:43–45

    Article  Google Scholar 

  • Harding K (1991) Molecular-stability of the ribosomal-RNA genes in Solanum tubersum plants recovered from slow growth and cryopreservation. Euphytica 55:141–146

    Article  CAS  Google Scholar 

  • Harding K (2004) Genetic integrity of cryopreserved plant cells. Cryo-lett 25:3–22

    Google Scholar 

  • Helliot B, Panis B, Poumay Y, Swenen R, Lepoivre P, Frison E (2002) Cryopreservation for the elimination of cucumber mosaic and banana streak viruses from banana (Musa spp.). Plant Cell Rep 20:1117–1122

    Article  CAS  Google Scholar 

  • Hirai D, Sakai A (1999) Cryopreservation of in vitro-grown meristems of potato (Solanum tuberosum L.) by encapsulation-vitrification. Potato Res 42:153–160

    Article  Google Scholar 

  • Loebenstein G (2001) Potato leafroll virus. In: Loebenstein G, Berger Ph H, Brunt A, Lawson RH (eds) Virus and virus-like diseases of potatoes and production of seed-potatoes. Kluwer, Dordrecht, pp 69–75

    Google Scholar 

  • Lozoya-Saldaña H, Dawson WO (1982) The use of constant and alternating temperature regimes and tissue culture to obtain PVS-free potato plants. Am Potato J 59:221–230

    Google Scholar 

  • Lozoya-Saldaña H, Merlin-Lara O (1984) Thermotherapy and tissue culture for elimination of Potato virus X (PVX) in Mexican potato cultivars resistant to late blight. Am Potato J 61:735–739

    Google Scholar 

  • Mellor FC, Stace-Smith R (1970) Virus stain differences in eradication of potato virus X and S. Phytopathology 60:1587–1590

    Google Scholar 

  • Mellor FC, Stace-Smith R (1971) Heat-stable strains of potato leafroll virus. Phytopathology 61:246–248

    Article  Google Scholar 

  • Morel G (1948) Recherches sur la culture associée de parasites obligatoires et de tissus végétaux. Ann Epiphyt 1:123–234

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco cell cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Sakai A, Kobayashi S, Oiyama I (1990) Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. Var. brasiliensis Tanaka) by vitrification. Plant Cell Rep 9:30–33

    Article  Google Scholar 

  • Sanchez GE, Slack SA, Dodds JH (1991) Response of selected Solanum species to virus eradication therapy. Am. Potato J 68:299–315

    Google Scholar 

  • Schäfer-Menuhr A, Müller E, Mix-Wagner G (1996) Cryopreservation: an alternative for the long-term of old potato varieties. Potato Res 39:507–513

    Article  Google Scholar 

  • Slack SA, Singh RP (1998) Control of viruses affecting potatoes through seed potato certification programs. In: Hadidi A, Khetarpal RK, Koganzawa H (eds) Plant virus disease control. APS, St. Paul, MN, pp 249–260

    Google Scholar 

  • Wang QC, Laamanen J, Uosukainen M, Valkonen JPT (2005) Cryopreservation of in vitro-grown shoot tips of raspberry (Rubus idaeus L.) by encapsulation-vitrification and encapsulation-dehydration. Plant Cell Rep 24:280–288

    Article  PubMed  CAS  Google Scholar 

  • Wang QC, Munir M, Li P, Gafny R, Sela I, Tanne E (2003) Elimination of grapevine virus A (GVA) by cryopreservation of in vitro-grown shoot tips of Vitis vinifera L. Plant Sci 165:321–327

    Article  CAS  Google Scholar 

  • Wang QC, Perl A (2006) Cryopreservation in Floricultural Plants. In: Teixeira da Silva JA (ed) Floriculture, Ornamental and Plant Biotechnology: Advances and Topical Issues. Global Science Books, London, UK, pp 524–539

    Google Scholar 

  • Waterworth HE, Hadidi A (1998) Economic losses due to plant viruses. In: Hadidi A, Khetarpal RK, Koganzawa H (eds) Plant virus disease control. APS, St. Paul, MN, pp 1–13

    Google Scholar 

Download references

Author information

Author notes

  1. Qiaochun Wang

    Present address: Department of Applied Biology, University of Helsinki, P.O. Box 27, 00014, Helsinki, Finland

Authors and Affiliations

  1. Plant Biotechnology Laboratory, Chengdu Center of Food Test and Analysis, Jingju Shi Lu 19, Chengdu, 610066, Sichuan, People’s Republic of China

    Qiaochun Wang, Yonghong Xie & Misa You

  2. Instittute of Crop Protection, Sichuan Academy of Agricultural Science, Chengdu, 610066, Sichuan, People’s Republic of China

    Yong Liu

Authors
  1. Qiaochun Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yong Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yonghong Xie
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Misa You
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Qiaochun Wang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wang, Q., Liu, Y., Xie, Y. et al. Cryotherapy of Potato Shoot Tips for Efficient Elimination of Potato Leafroll Virus (PLRV) and Potato Virus Y (PVY). Potato Res. 49, 119–129 (2006). https://doi.org/10.1007/s11540-006-9011-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11540-006-9011-4

Keywords

Search

Navigation