Abstract
Carboxymethytl pachymaram (CMP) was administered to HBV transgenic mice through abdominal injection. Lymphocytes were extracted from the spleens. MTT method was used to detect cytotoxicity of CMP. Dendritic cells (DCs) were separated from lymphocytes and incubated with granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4). Phenotypes of DC’s were assayed by flow cytometry (FCM). IL-12 released by DCs and IL-10 and IFN-γ produced by T cells in mixed lymphocyte reaction (MLR) were measured using ELISA. Results showed that CMP within the concentration of 0–500 μg/mL did not produce cytotoxicity to lymphocytes and could even increase DC phenotypes, and IL-12 level in HBV transgenic mice. It could also increase the secretion of IFN-γ, and inhibit the secretion of IL-10 inMLR. Thus it can up-regulate DC function.
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References
Barratt-Boyes S M, Zimmer M I, Harshyne L A, Meyer E M, Watkins S C, Capuano S3rd, Murphey-Corb M, Falo L DJr, Donnenberg A D (2000). Maturation and trafficking of monocyte-derived dendritic cells in monkeys: implications for dendritic cell-based vaccines. J Immunol, 164: 2487–2495
Beckebaum S, Cicinnati V R, Zhang X, Ferencik S, Frilling A, Grosse-Wilde H, Broelsch C E, Gerken G (2003). Hepatitis B virus-induced defect of monocyte-derived dendritic cells leads to impaired T helper type 1 response in vitro: mechanisms for viral immune escape. Immunology, 109: 487–495
Chen C X (2002). Effect of carboxymethyl pachymaram (CMP) on immunological function in mice. Edible Fungi, 4: 39–41 (in Chinese)
Dakic A, Shao Q X, D’Amico A, O’Keeffe M, Chen WF, Shortman K, Wu L (2004). Development of the dendritic cell system during mouse ontogeny. J Immunol, 172: 1018–1027
Duan H P, Hou A J, Lu F E, Huang J L (2005). Experimental study on the inhibitory effect of carboxymethytl pachymaram on hepatitis B virus expression from transfected cells. Chinese Journal of Experimental and Clinical Virology, 19(3): 290–292 (in Chinese)
Ebner S, Ratzinger G, Krosbacher B, Schmuth M, Weiss A, Reider D, Kroczek R A, Herold M, Heufler C, Fritsch P, Romani N (2001). Production of IL-12 by human monocyte-derived dendritic cells is optimal when the stimulus is given at the onset of maturation, and is further enhanced by IL-4. J Immunol, 166: 633–641
Goncalves L, Albarran B, Salmen S, Borges L, Fields H, Montes H, Soyano A, Diaz Y, Berrueta L (2004). The Nonresponse to hepatitis B vaccination is associated with impaired lymphocyte activation. Virology, 326: 20–28
Kunitani H, Shimizu Y, Murata H, Higuchi K, Watanabe A (2002). Phenotypic analysis of circulating and intrahepatic dendritic cell subsets in patients with chronic liver diseases. J Hepatol, 36: 734–741
Lunga T L, Saurwein-Teissla M, Parsonb W, Schönitzer D, Grubeck-Loebenstein B (2000). Unimpaired dendritic cells can be derived from monocytes in old age and can mobilize residual function in senescent T cells. Vaccine, 18: 1606–1612
MacDonald A S, Straw A D, Dalton N M, Pearce E J (2002). Cutting edge: Th2 response induction by dendritic cells: a role for CD40. J Immunol, 168: 537–540
Pollara G, Katz D R, Chain B M (2005). LIGHTing up Dendritic Cell Activation: Immune Regulation and Viral Exploitation. J Cell Physiol, 205(2): 161–162
Timares L, Safer K M, Qu B, Takashima A, Johnston S A (2003). Drug-inducible, dendritic cell-based genetic immunization. J Immunol, 170: 5483–5490
Wakkach A, Fournier N, Brun V, Breittmayer J, Cottrez F, Groux H (2003). Characterization of dendritic cells that induce tolerance and T regulatory cell differentiation in vivo. Immunity, 18: 605–617
Wang H M, Xia M, Xia D Z, Sun W, Pan H M, Xiang W L (2006). Anti-tumor effect of LPS and its mechanism. Zhejiang Journal of Integrated Traditional Chinese and Western Medicine, 16(5): 291–292 (in Chinese)
Yang J C, Sheng W H, Li L (1998). IL-2, TNF-α, IL-6, IFNγ/α production induced by carboxymethytl pachymaram (CMP) and pilot test. Immunological Journal, 14(3): 194–197 (in Chinese)
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Translated from Journal of Wuhan University (Natural Science Edition), 2006, 52(6): 778–782 [译自: 武汉大学学报(理学版)]
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Hou, A., Yang, Z., Huang, J. et al. Carboxymethytl pachymaram up-regulates dendritic cell function in hepatitis B virus transgenic mice. Front. Biol. China 3, 408–413 (2008). https://doi.org/10.1007/s11515-008-0058-2
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DOI: https://doi.org/10.1007/s11515-008-0058-2