Abstract
Spider dragline silk possesses impressive mechanical and biochemical properties. It is synthesized by a couple of major ampullate glands in spiders and comprises of two major structural proteins—spidroins 1 and 2. The relationship between structure and mechanical properties of spider silk is not well understood. Here, we modeled the complete process of the spider silk assembly using two new recombinant analogs of spidroins 1 and 2. The artificial genes sequence of the hydrophobic core regions of spidroin 1 and 2 have been designed using computer analysis of existing databases and mathematical modeling. Both proteins were expressed in Pichia pastoris and purified using a cation exchange chromatography. Despite the absence of hydrophilic N- and C-termini, both purified proteins spontaneously formed the nanofibrils and round micelles of about 1 μm in aqueous solutions. The electron microscopy study has revealed the helical structure of a nanofibril with a repeating motif of 40 nm. Using the electrospinning, the thin films with an antiparallel β-sheet structure were produced. In summary, we were able to obtain artificial structures with characteristics that are perspective for further biomedical applications, such as producing three-dimensional matrices for tissue engineering and drug delivery.
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Abbreviations
- PPII:
-
poly-l-proline
- FTIR:
-
Fourier transform infrared spectroscopy
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Acknowledgements
This work was supported in part by grants from Federal Agency of Science and Innovations (2007-3-1.3-22-01-453) to VD and RFBR (07-04-12172-ofi) to OS. The authors thank Ms Lisa Trifonova and Mr Askar Kuchumov for proofreading the manuscript.
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Vladimir G Bogush and Olga S Sokolova contributed equally to the paper.
Name(s) of guarantors of the work: Mikhail P Kirpichnikov, Vladimir G Debabov.
Meeting presentation: Russian–American Nanomedicine Workshop, Moscow, 10–12 Dec 2007.
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Bogush, V.G., Sokolova, O.S., Davydova, L.I. et al. A Novel Model System for Design of Biomaterials Based on Recombinant Analogs of Spider Silk Proteins. J Neuroimmune Pharmacol 4, 17–27 (2009). https://doi.org/10.1007/s11481-008-9129-z
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DOI: https://doi.org/10.1007/s11481-008-9129-z