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The PPR protein PDM1 is involved in the processing of rpoA pre-mRNA in Arabidopsis thaliana

  • Article
  • Plant Genetics
  • Published:
Chinese Science Bulletin

Abstract

Pentatricopeptide repeat (PPR) proteins, containing tandem repeats of degenerate 35 amino acid motifs, are important for post-transcriptional chloroplast gene expression. In this study, we report the characterization of a pigment-deficient mutant 1 (pdm1) in Arabidopsis, which displays the albino phenotype. PDM1 contains 5 PPR motifs followed by a PLS domain. The levels of plastid-encoded polymerase-dependent chloroplast genes were reduced dramatically, whereas those of nucleus-encoded polymerase-dependent chloroplast genes increased in the mutant. In addition, the pattern of rpoA pre-mRNA was altered and the rpoA transcript was absent in pdm1. Thus, these results suggest that PDM1 is required for processing of rpoA pre-mRNA in Arabidopsis.

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Authors and Affiliations

  1. School of Life Sciences, Lanzhou University, Lanzhou, 730000, China

    Hao Wu

  2. Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China

    LiXin Zhang

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  1. Hao Wu
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  2. LiXin Zhang
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Correspondence to LiXin Zhang.

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Wu, H., Zhang, L. The PPR protein PDM1 is involved in the processing of rpoA pre-mRNA in Arabidopsis thaliana . Chin. Sci. Bull. 55, 3485–3489 (2010). https://doi.org/10.1007/s11434-010-4040-4

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  • DOI: https://doi.org/10.1007/s11434-010-4040-4

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