Skip to main content
Log in

Precise editing of methylated cytosine in Arabidopsis thaliana using a human APOBEC3Bctd-Cas9 fusion

  • Letter to the Editor
  • Published:
Science China Life Sciences Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  • Ge, Z., Zheng, L., Zhao, Y., Jiang, J., Zhang, E.J., Liu, T., Gu, H., and Qu, L.J. (2019). Engineered xCas9 and SpCas9-NG variants broaden PAM recognition sites to generate mutations in Arabidopsis plants. Plant Biotechnol J 17, 1865–1867.

    Article  Google Scholar 

  • Jin, S., Fei, H., Zhu, Z., Luo, Y., Liu, J., Gao, S., Zhang, F., Chen, Y.H., Wang, Y., and Gao, C. (2020). Rationally designed APOBEC3B cytosine base editors with improved specificity. Mol Cell 79, 728–740.e6.

    Article  CAS  Google Scholar 

  • Komor, A.C., Kim, Y.B., Packer, M.S., Zuris, J.A., and Liu, D.R. (2016). Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Nature 533, 420–424.

    Article  CAS  Google Scholar 

  • Law, J.A., and Jacobsen, S.E. (2010). Establishing, maintaining and modifying DNA methylation patterns in plants and animals. Nat Rev Genet 11, 204–220.

    Article  CAS  Google Scholar 

  • Nabel, C.S., Jia, H., Ye, Y., Shen, L., Goldschmidt, H.L., Stivers, J.T., Zhang, Y., and Kohli, R.M. (2012). AID/APOBEC deaminases disfavor modified cytosines implicated in DNA demethylation. Nat Chem Biol 8, 751–758.

    Article  CAS  Google Scholar 

  • Stroud, H., Do, T., Du, J., Zhong, X., Feng, S., Johnson, L., Patel, D.J., and Jacobsen, S.E. (2014). Non-CG methylation patterns shape the epigenetic landscape in Arabidopsis. Nat Struct Mol Biol 21, 64–72.

    Article  CAS  Google Scholar 

  • Stroud, H., Greenberg, M.V.C., Feng, S., Bernatavichute, Y.V., and Jacobsen, S.E. (2013). Comprehensive analysis of silencing mutants reveals complex regulation of the Arabidopsis methylome. Cell 152, 352–364.

    Article  CAS  Google Scholar 

  • Wang, X., Li, J., Wang, Y., Yang, B., Wei, J., Wu, J., Wang, R., Huang, X., Chen, J., and Yang, L. (2018). Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol 36, 946–949.

    Article  CAS  Google Scholar 

  • Zeng, D., Li, X., Huang, J., Li, Y., Cai, S., Yu, W., Li, Y., Huang, Y., Xie, X., Gong, Q., et al. (2020). Engineered Cas9 variant tools expand targeting scope of genome and base editing in rice. Plant Biotechnol J 18, 1348–1350.

    Article  Google Scholar 

  • Zhang, R., Chen, S., Meng, X., Chai, Z., Wang, D., Yuan, Y., Chen, K., Jiang, L., Li, J., and Gao, C. (2020). Generating broad-spectrum tolerance to ALS-inhibiting herbicides in rice by base editing. Sci China Life Sci doi: https://doi.org/10.1007/s11427-020-1800-5.

  • Zong, Y., Song, Q., Li, C., Jin, S., Zhang, D., Wang, Y., Qiu, J.L., and Gao, C. (2018). Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A. Nat Biotechnol 36, 950–953.

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by the National Natural Science Foundation of China (31960138), the Natural Science Foundation of Jiangxi Province (20171ACB20001), and the National Key Research and Development Program of China (2016YRD0102003).

Author information

Authors and Affiliations

Authors

Corresponding authors

Correspondence to Xiaofeng Cao or Dong Wang.

Additional information

Compliance and ethics

The author(s) declare that they have no conflict of interest.

Supporting Information

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Liu, ZH., Tang, S., Hu, W. et al. Precise editing of methylated cytosine in Arabidopsis thaliana using a human APOBEC3Bctd-Cas9 fusion. Sci. China Life Sci. 65, 219–222 (2022). https://doi.org/10.1007/s11427-021-1970-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11427-021-1970-x

Navigation