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Allen, F., Crepaldi, L., Alsinet, C., Strong, A.J., Kleshchevnikov, V., De Angeli, P., Páleníková, P., Khodak, A., Kiselev, V., Kosicki, M., et al. (2018). Predicting the mutations generated by repair of Cas9-induced double-strand breaks. Nat Biotechnol 37, 64–72.
Anzalone, A.V., Randolph, P.B., Davis, J.R., Sousa, A.A., Koblan, L.W., Levy, J.M., Chen, P.J., Wilson, C., Newby, G.A., Raguram, A., et al. (2019). Search-and-replace genome editing without double-strand breaks or donor DNA. Nature 576, 149–157.
Chen, W., McKenna, A., Schreiber, J., Haeussler, M., Yin, Y., Agarwal, V., Noble, W.S., and Shendure, J. (2019). Massively parallel profiling and predictive modeling of the outcomes of CRISPR/Cas9-mediated double-strand break repair. Nucleic Acids Res 47, 7989–8003.
Chen, Y., Wang, Z., Ni, H., Xu, Y., Chen, Q., and Jiang, L. (2017). CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Sci China Life Sci 60, 520–523.
Gaudelli, N.M., Komor, A.C., Rees, H.A., Packer, M.S., Badran, A.H., Bryson, D.I., and Liu, D.R. (2017). Programmable base editing of A*T to G-C in genomic DNA without DNA cleavage. Nature 551, 464–471.
Jin, S., Zong, Y., Gao, Q., Zhu, Z., Wang, Y., Qin, P., Liang, C., Wang, D., Qiu, J.L., Zhang, F., et al. (2019). Cytosine, but not adenine, base editors induce genome-wide off-target mutations in rice. Science 364, 292–295.
Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J.A., and Charpentier, E. (2012). A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science 337, 816–821.
Komor, A.C., Kim, Y.B., Packer, M.S., Zuris, J.A., and Liu, D.R. (2016). Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Nature 533, 420–424.
Mallapaty, S. (2019). Australian gene-editing rules adopt ‘middle ground’. Nature https://doi.org/10.1038/d41586-019-01282-8.
Miller, S.M., Wang, T., Randolph, P.B., Arbab, M., Shen, M.W., Huang, T. P., Matuszek, Z., Newby, G.A., Rees, H.A., and Liu, D.R. (2020). Continuous evolution of SpCas9 variants compatible with non-G PAMs. Nat Biotechnol 38, 471–481.
Shen, M.W., Arbab, M., Hsu, J.Y., Worstell, D., Culbertson, S.J., Krabbe, O., Cassa, C.A., Liu, D.R., Gifford, D.K., and Sherwood, R.I. (2018). Predictable and precise template-free CRISPR editing of pathogenic variants. Nature 563, 646–651.
Walton, R.T., Christie, K.A., Whittaker, M.N., and Kleinstiver, B.P. (2020). Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants. Science 368, 290–296.
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This work was supported by the National Transgenic Science and Technology Program (2019ZX08010-003) and National Natural Science Foundation of China (31872933).
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A patent was filed to patent office in China, and key authors were listed as inventors. L.J. and H.L. designed the experiments; W.Y., W.Q. and Y.L. performed most of the experiments with the help from other authors; H.L. supervised the project at Kingagroot and L.J. supervised the project at CAU; H.L. and L.J. wrote the manuscript; L.J. conceived the idea.
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Yang, W., Qi, W., Li, Y. et al. Programmed sequential cutting endows Cas9 versatile base substitution capability in plants. Sci. China Life Sci. 64, 1025–1028 (2021). https://doi.org/10.1007/s11427-020-1798-4
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DOI: https://doi.org/10.1007/s11427-020-1798-4