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Profiling of lysine-acetylated proteins in human urine

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Abstract

A biomarker is a measurable indicator associated with changes in physiological state or disease. In contrast to the blood which is under homeostatic controls, urine reflects changes in the body earlier and more sensitively, and is therefore a better biomarker source. Lysine acetylation is an abundant and highly regulated post-translational modification. It plays a pivotal role in modulating diverse biological processes and is associated with various important diseases. Enrichment or visualization of proteins with specific post-translational modifications provides a method for sampling the urinary proteome and reducing sample complexity. In this study, we used anti-acetyllysine antibody-based immunoaffinity enrichment combined with high-resolution mass spectrometry to profile lysine-acetylated proteins in normal human urine. A total of 629 acetylation sites on 315 proteins were identified, including some very low-abundance proteins. This is the first proteome-wide characterization of lysine acetylation proteins in normal human urine. Our dataset provides a useful resource for the further discovery of lysine-acetylated proteins as biomarkers in urine.

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Acknowledgements

This work was supported by the National Key Research and Development Program of China (2018YFC0910202, 2016YFC1306300), the Beijing Natural Science Foundation (7172076), the Beijing cooperative construction project (110651103), the Beijing Normal University (11100704), the Peking Union Medical College Hospital (2016-2.27).

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Gene Engineering Drug and Biotechnology Beijing Key Laboratory, Beijing Normal University, Beijing, 100875, China

    Weiwei Qin, Ting Wang, He Huang & Youhe Gao

  2. Department of Anesthesiology, Qingdao Municipal Hospital, Qingdao, 266071, China

    Weiwei Qin

Authors
  1. Weiwei Qin
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  2. Ting Wang
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  3. He Huang
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  4. Youhe Gao
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Corresponding author

Correspondence to Youhe Gao.

Additional information

Compliance and ethics The author(s) declare that they have no conflict of interest. The consent procedure and the study protocol were approved by the Institutional Review Board of the Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences. (Project No. 007-2014). Written informed consent was obtained from each subject prior to the study.

Supporting Information

Figure S1 Overview of lysine acetylation in urine proteome of healthy humans.

Table S1 Acetylated peptides (Peptide-Spectrum Matches FDR<0.1%)

Table S2 Acetylated proteins (unique peptides≥1) with 629 Kac sites (AScore≥59) in normal human urine

Supporting Spectra S1 The represent MS/MS spectra for 761 lysine acetylated peptides.

Supporting Spectra S2 The remaining MS/MS spectra for 761 lysine acetylated peptides.

The supporting information is available online at http://life.scichina.com and https://link.springer.com. The supporting materials are published as submitted, without typesetting or editing. The responsibility for scientific accuracy and content remains entirely with the authors.

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Qin, W., Wang, T., Huang, H. et al. Profiling of lysine-acetylated proteins in human urine. Sci. China Life Sci. 62, 1514–1520 (2019). https://doi.org/10.1007/s11427-017-9367-6

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