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Detecting CD20-Rituximab specific interactions on lymphoma cells using atomic force microscopy

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Abstract

Elucidating the underlying mechanisms of cell physiology is currently an important research topic in life sciences. Atomic force microscopy methods can be used to investigate these molecular mechanisms. In this study, single-molecule force spectroscopy was used to explore the specific recognition between the CD20 antigen and anti-CD20 antibody Rituximab on B lymphoma cells under near-physiological conditions. The CD20-Rituximab specific binding force was measured through tip functionalization. Distribution of CD20 on the B lymphoma cells was visualized three-dimensionally. In addition, the relationship between the intramolecular force and the molecular extension of the CD20-Rituximab complex was analyzed under an external force. These results facilitate further investigation of the mechanism of Rituximab’s anti-cancer effect.

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Authors and Affiliations

  1. State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, 110016, China

    Mi Li, LianQing Liu, Ning Xi, YueChao Wang, ZaiLi Dong & GuangYong Li

  2. Department of Electrical and Computer Engineering, Michigan State University, East Lansing, 48824, USA

    Ning Xi

  3. Department of Electrical and Computer Engineering, University of Pittsburgh, Pittsburgh, 15261, USA

    GuangYong Li

  4. Department of Lymphoma Affiliated Hospital of Military Medical Academy of Sciences, Beijing, 100071, China

    XiuBin Xiao & WeiJing Zhang

  5. Graduate University of Chinese Academy of Sciences, Beijing, 100049, China

    Mi Li

  6. State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China

    Mi Li

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  1. Mi Li
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Correspondence to LianQing Liu, Ning Xi or WeiJing Zhang.

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Li, M., Liu, L., Xi, N. et al. Detecting CD20-Rituximab specific interactions on lymphoma cells using atomic force microscopy. Sci. China Life Sci. 53, 1189–1195 (2010). https://doi.org/10.1007/s11427-010-4070-9

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  • DOI: https://doi.org/10.1007/s11427-010-4070-9

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