Abstract
This work studied the transcriptional patterns of three antibacterial genes, attacin, defensin and cecropin, during the development of Musca domestica. Quantitative analysis by real-time PCR was performed on mRNA levels in different development stages and challenged 3rd-instar larva at different time points after challenge of Musca domestica. The results revealed a predominance of the transcripts of all three genes during the 3rd-instar larvae and the adults. In the meanwhile, it revealed the greatest increase in mRNA. The transcript levels increased to 801 times, 1009 times and 2500 times respectively for cecropin, attacin and defensin in 3rd-instar larvae after challenging susceptible bacterium. The results suggested that the transcriptional patterns of Musca domestica antibacterial genes were different during the different growth stages as well as the microbial challenge encountered in 3rd-instar larvae.
Similar content being viewed by others
Reference
Silverman N, Maniatis T. NF-κB signalling pathways in mammalian and insect immunity. Genes, 2001, 15: 2321–2342 10.1101/gad.909001, 1:CAS:528:DC%2BD3MXnsV2hs7o%3D
Hoffmann J A, Reichhart J M. Drosophila innate immunity: An evolutionary perspective. Nat Immunol, 2002, 3: 121–126 11812988, 10.1038/ni0202-121, 1:CAS:528:DC%2BD38XhtVygtbc%3D
Boman H G. Gene-encoded peptide antibiotics and the concept of innate immunity: an update review. Scand J Immunol, 1998, 48: 15–25 9714406, 10.1046/j.1365-3083.1998.00343.x, 1:CAS:528:DyaK1cXlsVKltr4%3D
Naitza S, Ligoxygakis P. Antimicrobial defences in Drosophila: The story so far. Mol Immunol, 2004, 40: 887–896 14698227, 10.1016/j.molimm.2003.10.008, 1:CAS:528:DC%2BD3sXpvFensrY%3D
Mittapalli O, Shukle R H, Sardesai N, et al. Expression patterns of antibacterial genes in the Hessian fly. J Insect Physiol, 2006, 52: 1143–1152 17070830, 10.1016/j.jinsphys.2006.08.007, 1:CAS:528:DC%2BD28Xht1CqtrfI
Choe K M, Werner T, Stoven S, et al. Requirement for a peptidoglycan recognition protein PGRP in Relish activation and antibacterial immune responses in Drosophila. Science, 2002, 296: 359–362 11872802, 10.1126/science.1070216, 1:CAS:528:DC%2BD38XjtVWhu7g%3D
An R J, Xing C L, Wang Z Y, et al. Feeding technique of Musca domestica linnaous. J Zhelimu Anim Husbandry Coll, 1999, 9: 49–52
Chaves-Pozo E, Liarte S, Fernández-Alacid L, et al. Pattern of expression of immune-relevant genes in the gonad of a teleost, the gilthead seabream (Sparus aurata L). Mol Immunol, 2008, 45: 2998–3011 18325594, 10.1016/j.molimm.2008.01.018, 1:CAS:528:DC%2BD1cXksFKkt7w%3D
Imler J L, Bulet P, Kabelitz D, et al. Antimicrobial peptides in Drosophila: structures, activities and gene regulation. Chem Immunol Allergy, 2005, 86: 1–21 15976485, 10.1159/000086648, 1:CAS:528:DC%2BD2MXhtVensr%2FJ
Li B, Calvo E, Marinotti O, et al. Characterization of the C-type lysozyme gene family in Anopheles gambiae. Gene, 2005, 360: 131–139 16137842, 10.1016/j.gene.2005.07.001, 1:CAS:528:DC%2BD2MXhtFaktbvI
Subramanyam S, Sardesai N, Puthoff D P, et al. Expression of two wheat defense-response genes, Hfr-1 and Wci-1, under biotic and abiotic stresses. Plant Sci, 2006, 170: 90–103 10.1016/j.plantsci.2005.08.006, 1:CAS:528:DC%2BD2MXhtFektbvO
Jin X B, Zhu J Y, Ma Y, et al. The expression of anti-bacterial peptide cecropin gene in COS-7 cells and the preliminary study on the activities of its gene product. Chin J Zooneses (In Chinese), 2007, 23: 566–568 1:CAS:528:DC%2BD1cXpvVGktbo%3D
Li Y L. Human Parasitology(In Chinese). 6th ed. Beijing: People’s Medical Publishing House, 2004, 229.
JIN X B, WANG Y, ZHU J Y, et al. Study on expression analysis of antibacterial peptide genes at different development stages of Musca domestica. Chin J Parasitol Parasit Dis (in Chinese), 2008, 26: 1-0187–0190
Wang Y, Jin X B, Zhu J Y, et al. Expression pattern of attacin gene in larvae of Musca domestica. Chin Trop Med (In Chinese), 2007, 7: 862–864
Author information
Authors and Affiliations
Corresponding authors
Additional information
Supported by the National Natural Science Foundation of China (Grant No. 30671832) and the Key Science and Technology Program of Guangzhou (Grant No. 2005Z32E0211)
Rights and permissions
About this article
Cite this article
Wang, Y., Jin, X., Zhu, J. et al. Expression pattern of antibacterial genes in the Musca domestica. SCI CHINA SER C 52, 823–830 (2009). https://doi.org/10.1007/s11427-009-0121-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11427-009-0121-5