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A simple quantitation method for benzoylecgonine from oral fluid, blood, and urine samples used for determining 22 illicit and licit drugs by GC–MS with liquid–liquid extraction

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Abstract

The analytical system used for determination of 22 illicit and licit drugs by gas chromatography–mass spectrometry following liquid–liquid extraction has been extended with an additional extraction step with n-butyl acetate:CH2Cl2 = 1:8 to determine benzoylecgonine from the same oral fluid, blood, or urine samples. The extraction recovery of benzoylecgonine was 39.7% for oral fluid, 14.9% for blood, and 26.7% for urine; the cutoffs were 25, 8.0, and 8.0 ng/ml, respectively. The method was fully validated and proved suitable in further proficiency tests and for analysis of 2738 oral fluid, 197 blood, and 1298 urine samples.

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Acknowledgments

This work was financially supported by TREN-05-PF6TR-S07. 61320-518404-DRUID. The authors thank to Pirjo Lillsunde, Kaarina Langel, Kari Ariniemi, and Teemu Gunnar (National Institute for Health and Welfare, Helsinki, Finland) for their help and advice in adaptation of the LLE and GC–MS analysis system to our laboratory. The authors also thank Tünde Benkő and Edit Kopasz for technical assistance.

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Authors and Affiliations

  1. Department of Forensic Medicine, University of Szeged, Kossuth L. sgt. 40, P.O. Box 427, Szeged, 6724, Hungary

    László Institóris, Zsófia Árok, Éva Kereszty & Tibor Varga

  2. TEVA Pharmaceutical Works, Táncsics Mihály út 82, Gödöllő, 2100, Hungary

    Vilmos Angyal

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  1. László Institóris
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  2. Vilmos Angyal
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  3. Zsófia Árok
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Correspondence to László Institóris.

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Institóris, L., Angyal, V., Árok, Z. et al. A simple quantitation method for benzoylecgonine from oral fluid, blood, and urine samples used for determining 22 illicit and licit drugs by GC–MS with liquid–liquid extraction. Forensic Toxicol 30, 59–65 (2012). https://doi.org/10.1007/s11419-011-0128-z

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  • DOI: https://doi.org/10.1007/s11419-011-0128-z

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