Abstract
A simple and reliable method to determine acephate and methamidophos in mammalian tissues is presented. The method includes solid-phase extraction of tissue extracts with active carbon cartridges followed by gas chromatography–mass spectrometry analysis. During the study, a matrix effect was observed especially at low concentrations of acephate and methamidophos in serum and in brain. To minimize the effect, we prepared calibration curves with relatively short ranges. The validation data, such as the linearity of calibration curves, limits of detection, and coefficients of variation for recovery rates, were generally satisfactory. The present method is useful for determination of acephate and methamidophos in mammalian tissues because of its simplicity and speed, keeping in mind the presence of the matrix effect.
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Acknowledgments
This work was supported by Grant-in-Aid for Scientific Research (22930007) and Grant-in-Aid for Graduate Students, Hyogo College of Medicine.
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Adachi, N., Kinoshita, H., Nishiguchi, M. et al. Determination of acephate and methamidophos in tissues: appearance of matrix effect in gas chromatography–mass spectrometry. Forensic Toxicol 29, 159–162 (2011). https://doi.org/10.1007/s11419-011-0110-9
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DOI: https://doi.org/10.1007/s11419-011-0110-9