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A new 2H-benzindazole compound from Alternaria alternata Shm-1, an endophytic fungus isolated from the fresh wild fruit of Phellinus igniarius

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Abstract

Endophytic fungi have been shown in recent years to produce a series of bioactive secondary metabolites. Several endophytic fungi were isolated from the fresh wild body of Phellinus igniarius, and initially evaluated for their antimicrobial activity. Among which, Shm-1 extract showed moderate inhibitory activity against Clavibacter michiganense and the fungus was identified to be Alternaria alternata Shm-1 through the comparison of morphological characteristics and the sequence of the rDNA ITS with those of other Alternaria species. A new 2H-benzindazole derivative, alterindazolin A (1), has been isolated from cultures of the endophyte Alternaria alternata Shm-1. Its structure was characterized as 1-benzyl-5-p-hydroxy-phenyloxygen-benz[e]indazole by spectroscopic data analysis including 1D NMR, 2D NMR and MS spectrum.

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Acknowledgements

The authors are grateful to Prof. Z. G. Yue for the collection of fresh fruit of P. igniarius. The project was financially supported by the National Natural Science Foundation of China (NNSFC; Grant nos: 81260477 and 81560567).

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    Authors and Affiliations

    1. College of Pharmacy, Ningxia Engineering and Technology Research Center of Modern Hui Medicine, Key Laboratory of Hui Ethnic Medicine Modernization, Ministry of education, Ningxia Medical University, Yinchuan, 750004, People’s Republic of China

      Xiuli Wu, Sa Wang, Cheng Liu, Caifang Zhang & Jinjing Guo

    2. Beijing Key Laboratory of Bioactive Substances and Functional Foods, Beijing Union University, Beijing, 100191, People’s Republic of China

      Xiaoya Shang

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    1. Xiuli Wu
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    Correspondence to Xiuli Wu or Xiaoya Shang.

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    Sa Wang contributed equally to this work.

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    Wu, X., Wang, S., Liu, C. et al. A new 2H-benzindazole compound from Alternaria alternata Shm-1, an endophytic fungus isolated from the fresh wild fruit of Phellinus igniarius. J Nat Med 73, 620–626 (2019). https://doi.org/10.1007/s11418-019-01291-x

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