Abstract
In this study, we aimed to determine whether Sanggenon G, an active compound isolated from the root bark of Morus alba, exhibited enhanced anti-immobility activity with the addition of the α2-antagonist yohimbine in rats subjected to forced swim test (FST)-induced depression. Fluoxetine (a selective serotonin reuptake inhibitor) treatment in rats reduced the immobility time, and pretreatment with yohimbine significantly enhanced the antidepressant-like behavior of fluoxetine at 5, 10 and 20 mg/kg. Similarly, Sanggenon G significantly decreased the immobility time, reducing immobility by a maximum of 43.9 % when treated at a dose of 20 mg/kg. Furthermore, pretreatment with yohimbine significantly enhanced the antidepressant-like behavior of Sanggenon G at 5 and 10 mg/kg. Our findings suggest that the antidepressant-like effect of Sanggenon G could be facilitated by concomitant use of the α2-antagonist. Further studies are needed to evaluate the potential of Sanggenon G as an alternative therapeutic approach for the treatment of depression.
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References
Muguruza C, Rodriguez F, Rozas I, Meana JJ, Uriguen L, Callado LF (2013) Antidepressant-like properties of three new alpha2-adrenoceptor antagonists. Neuropharmacology 65:13–19
Rodriguez F, Rozas I, Ortega JE, Erdozain AM, Meana JJ, Callado LF (2008) Guanidine and 2-aminoimidazoline aromatic derivatives as alpha2-adrenoceptor antagonists. 2. Exploring alkyl linkers for new antidepressants. J Med Chem 51:3304–3312
Rozas I (2009) Improving antidepressant drugs: update on recently patented compounds. Expert Opin Ther Pat 19:827–845
Bremner JD (1995) A double-blind comparison of Org 3770, amitriptyline, and placebo in major depression. J Clin Psychiatry 56:519–525
Asano N, Yamashita T, Yasuda K, Ikeda K, Kizu H, Kameda Y, Kato A, Nash RJ, Lee HS, Ryu KS (2001) Polyhydroxylated alkaloids isolated from mulberry trees (Morusalba L.) and silkworms (Bombyx mori L.). J Agric Food Chem 49:4208–4213
Nade VS, Kawale LA, Naik RA, Yadav AV (2009) Adaptogenic effect of Morus alba on chronic footshock-induced stress in rats. Indian J Pharmacol 41:246–251
Lee MS, Park WS, Kim YH, Kwon SH, Jang YJ, Han D, Morita K, Her S (2013) Antidepressant-like effects of Cortex Mori Radicis extract via bidirectional phosphorylation of glucocorticoid receptors in the hippocampus. Behav Brain Res 236:56–61
Lim DW, Kim YT, Park JH, Baek NI, Han D (2014) Antidepressant-like effects of the ethyl acetate soluble fraction of the root bark of Morus alba on the immobility behavior of rats in the forced swim test. Molecules 19:7981–7989
Nade VS, Yadav AV (2010) Anti-stress effect of ethyl acetate soluble fraction of Morus alba in chronic restraint stress. Pharm Biol 48:1038–1046
Sattayasai J, Tiamkao S, Puapairoj P (2008) Biphasic effects of Morus alba leaves green tea extract on mice in chronic forced swimming model. Phytother Res 22:487–492
Jung JW, Ko WM, Park JH, Seo KH, Oh EJ, Lee DY, Lee DS, Kim YC, Lim DW, Han D, Baek NI (2015) Isoprenylated flavonoids from the root bark of Morus alba and their hepatoprotective and neuroprotective activities. Arch Pharm Res 38:2066–2075
Lim DW, Jung JW, Park JH, Baek NI, Kim YT, Kim IH, Han D (2015) Antidepressant-like effects of sanggenon G, isolated from the root bark of Morus alba, in rats: involvement of the serotonergic system. Biol Pharm Bull 38:1772–1778
Cryan JF, Markou A, Lucki I (2002) Assessing antidepressant activity in rodents: recent developments and future needs. Trends Pharmacol Sci 23:238–245
Petit-Demouliere B, Chenu F, Bourin M (2005) Forced swimming test in mice: a review of antidepressant activity. Psychopharmacology 177:245–255
Renard CE, Dailly E, David DJ, Hascoet M, Bourin M (2003) Monoamine metabolism changes following the mouse forced swimming test but not the tail suspension test. Fundam Clin Pharmacol 17:449–455
Sanacora G, Berman RM, Cappiello A, Oren DA, Kugaya A, Liu N, Gueorguieva R, Fasula D, Charney DS (2004) Addition of the alpha2-antagonist yohimbine to fluoxetine: effects on rate of antidepressant response. Neuropsychopharmacology 29:1166–1171
Hopwood SE, Stamford JA (2001) Noradrenergic modulation of serotonin release in rat dorsal and median raphe nuclei via alpha(1) and alpha(2A) adrenoceptors. Neuropharmacology 41:433–442
Yanpallewar SU, Fernandes K, Marathe SV, Vadodaria KC, Jhaveri D, Rommelfanger K, Ladiwala U, Jha S, Muthig V, Hein L, Bartlett P, Weinshenker D, Vaidya VA (2010) Alpha2-adrenoceptor blockade accelerates the neurogenic, neurotrophic, and behavioral effects of chronic antidepressant treatment. J Neurosci 30:1096–1109
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This study was supported by a grant from the Korea Food Research Institute.
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Lim, D.W., Baek, NI., Kim, Y.T. et al. Enhanced anti-immobility effects of Sanggenon G isolated from the root bark of Morus alba combined with the α2-antagonist yohimbine in the rat forced swim test. J Nat Med 70, 679–682 (2016). https://doi.org/10.1007/s11418-016-0975-3
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DOI: https://doi.org/10.1007/s11418-016-0975-3