Abstract
A quantitative 1H-NMR method (qHNMR) was used to measure paeoniflorin content in Paeoniae radix (dried root of Paeonia lactiflora), of which paeoniflorin is a major component. The purity of paeoniflorin was calculated from the ratio of the intensity of the H-9 signal at δ 5.78 ppm of paeoniflorin to that of a hexamethyldisilane (HMD) signal at 0 ppm. The concentration of HMD was corrected with SI traceability by using bisphenol A of certified reference material (CRM) grade. The paeoniflorin content in 2 separate samples of Paeoniae radix was determined by qHNMR and was found to be 2.15 and 2.45 %. We demonstrated that this method is useful for quantitative analysis of crude drugs.
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References
Shibata S, Nakahara M (1963) Studies on the constituents of Japanese and Chinese crude drugs. VIII. Paeoniflorin, a glucoside of Chinese paeony root. Chem Pharm Bull 11(3):372–378
Harada M (1989) Experimental methodology on pharmacological studies on crude drugs. J Tradit Sino Jpn Med 107:1–16
Hikino H (1985) Oriental medical plants. In: Wagner H, Hikino H, Farnsworth NR (eds) Economic and medicinal plant research, vol. 1. London Academic Press, London
Yamahara J, Yamada T, Kimura H, Sawada T, Fujimura H (1982) Biologically active principles of crude drugs. II. Anti-allergic principles in “Shoseiryu-To” anti-inflammatory properties of paeoniflorin and its derivatives. J Pharmacobiodyn 5(11):921–929
Takagi K, Harada M (1969) Pharmacological studies on herb paeony root. II. Anti-inflammatory effect, inhibitory effect on gastric juice secretion, preventive effect on stress ulcer, antidiuretic effect of paeoniflorin and combined effects with licorice component Fm 100. Yakugaku Zasshi 89(7):887–892
Sugishita E, Amagaya S, Ogihara Y (1984) Studies on the combination of Glycyrrhizae Radix in Shakuyakukanzo-To. J Pharmacobiodyn 7(7):427–435
Ministry of Health and Welfare (2000) The Japanese Pharmacopeia, 15th edn. Japan, pp 1223–1224
Pauli GF, Jaki BU, Lankin DC (2005) Quantitative 1H NMR: development and potential of a method for natural products analysis. J Nat Prod 68:133–149
Tahara M, Sugimoto N, Suematsu T, Arifuku K, Saito T, Ihara T, Yoshida Y, Tada A, Kubota R, Shimizu K, Yamazaki T, Tanamoto K, Nakazawa H, Nishimura T (2009) Quality control of organophosphorous isoxathion oxon based on qNMR. Jpn J Food Chem Safety 16:28–33
Hasada K, Yoshida T, Yamazaki T, Sugimoto N, Nishimura T, Nagatsu A, Mizukami H (2010) Quantitative determination of atractylon in Atractylodis Rhizoma and Atractylodis Lanceae Rhizoma by 1H-NMR spectrometry. J Nat Med 64:161–164
Hasada K, Yoshida T, Yamazaki T, Sugimoto N, Nishimura T, Nagatsu A, Mizukami H (2011) Application of 1H-NMR spectroscopy to validation of berberine alkaloid reagents and to chemical evaluation of Coptidis Rhizoma. J Nat Med 65:262–267
Saito T, Ihara T, Koike M, Kinugasa S, Fujimine Y, Nose K, Hirai T (2009) A new traceability scheme for the development of international system-traceable persistent organic pollutant reference materials by quantitative nuclear magnetic resonance. Accredit Qual Assur 14:79–86
Yoo J-S, Song M-C, Ahn E-M, Lee Y-H, Rho Y-D, Baek N-I (2006) Quantitative analysis of paeoniflorin from Paeonia lactiflora using 1H-NMR. Nat Prod Sci 12(4):237–240
Acknowledgments
We thank Dr. N. Sugimoto of the National Institute of Health Sciences for kind advice on measurement using qHNMR.
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Tanaka, R., Yamazaki, M., Hasada, K. et al. Application of quantitative 1H-NMR method to determination of paeoniflorin in Paeoniae radix. J Nat Med 67, 657–661 (2013). https://doi.org/10.1007/s11418-012-0711-6
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DOI: https://doi.org/10.1007/s11418-012-0711-6