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Isolation of cytochrome P450 3A (CYP3A) inhibitors from Hyuganatsu, Citrus tamurana Hort.

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Abstract

The present study was conducted to identify cytochrome P450 3A (CYP3A) inhibitory components of Hyuganatsu, Citrus tamurana Hort., by investigating the effects on midazolam 1′-hydroxylase activity of human liver microsomes. As a consequence, limonin and nomilin were identified as CYP3A inhibitors from the endocarp of Hyuganatsu.

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Acknowledgements

This work was financially supported by the Promotion of Evolutional Science and Technology in Miyazaki Prefecture from the Miyazaki 21st Century Strategy Promotion Foundation. The authors thank Prof. Keiji Wada, Health Sciences University of Hokkaido, and Associate Prof. Yasuhiro Tezuka, Toyama Medical and Pharmaceutical University for the generous gift of nomilin and its NMR spectra, respectively.

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Authors and Affiliations

  1. School of Pharmaceutical Sciences, Kyushu University of Health and Welfare, 1714-1 Yoshino-cho, Nobeoka 882-8508, Japan

    Shinzo Hosoi, Eri Shimizu, Noriyuki Usami, Ikuo Yamamoto & Akiyo Sakushima

  2. Department of Pharmacy, Miyazaki Medical College Hospital, 5200 Kihara, Kiyotake-cho, Miyazaki-gun, Miyazaki 889-1692, Japan

    Kazuhiko Arimori, Manabu Okumura & Muneaki Hidaka

  3. Department of Nutrition Science, Faculty of Health and Nutrition Sciences, Minami Kyushu University, 5-1-2 Kirishima, Miyazaki 880-0032, Japan

    Mitsuko Yamada

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  1. Shinzo Hosoi
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  2. Eri Shimizu
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  3. Noriyuki Usami
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  4. Ikuo Yamamoto
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  5. Kazuhiko Arimori
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  7. Muneaki Hidaka
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  8. Mitsuko Yamada
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  9. Akiyo Sakushima
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Correspondence to Shinzo Hosoi.

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Hosoi, S., Shimizu, E., Usami, N. et al. Isolation of cytochrome P450 3A (CYP3A) inhibitors from Hyuganatsu, Citrus tamurana Hort.. J Nat Med 60, 240–242 (2006). https://doi.org/10.1007/s11418-006-0031-9

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  • DOI: https://doi.org/10.1007/s11418-006-0031-9

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