Skip to main content

Advertisement

SpringerLink
Log in

Beneficial regulation of type I collagen and matrixmetalloproteinase-1 expression by estrogen, progesterone, and its combination in skin fibroblasts.

  • Published:
Journal of the American Aging Association Aims and scope Submit manuscript

Abstract

There is impaired wound healing and loss of type I collagen in skin aging, which can be improved by topical estrogen in vivo. The goal of this study was to determine the effects of estrogen, and progesterone and a combination of estrogen and progesterone as well, on the proliferation and the expression of type I collagen and matrixmetalloprotienase-1 (MMP-1, degrades collagen) in dermal fibroblasts (cells that synthesize collagen and MMP-1) in-vitro. Estrogen, progesterone, and its combination similarly and significantly inhibited cell proliferation and MMP-1 protein levels, and simultaneously stimulated type I collagen expression in the fibroblasts, indicating beneficial modulation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Vaillant, L, and Callens, A: Hormone Replacement Treatment and Skin Aging. Therapie, 51:67–70, 1996.

    PubMed  CAS  Google Scholar 

  2. Mori, Y, Hatamochi, A, Arakawa, M, and Ueki, H: Reduced Expression of mRNA for Transforming Growth Factor Beta (TGF beta) and TGF Beta Receptors I and II and Decreased TGF Beta Binding to the Receptors in in Vitro-Aged Fibroblasts. Arch Dermatol Res, 290:158–62, 1998.

    Article  PubMed  CAS  Google Scholar 

  3. Ashcroft, G, Dodsworth, J, VanBoxtel, E, Tarnuzzer, R, Horan, M, Schultz, G, and Ferguson, M: Estrogen Accelerates Cutaneous Wound Healing Associated With an increase in TGF-β1 Levels. Nat Med, 3: 1209–1215, 1997.

    Article  PubMed  CAS  Google Scholar 

  4. Ashcroft, GS, Greenwell-Wild, T, Horan, MA, Wahl, SM, Ferguson, M WJ: Topical Estrogen Accelerates Cutaneous Wound Healing in Aged Humans Associated with an Altered Inflammatory Response. Am J Pathol, 155:1137–1146, 1999.

    PubMed  CAS  Google Scholar 

  5. Uitto, J, Fazio, MJ, and Olsen, D: Molecular mechanisms of cutaneous aging. J Am Acad Dermatol, 21: 614–22, 1989.

    Article  PubMed  CAS  Google Scholar 

  6. Pierard G, Letawe C, Dowlati A, and Pierard-Franchimont C: Effect of Hormone Replacement Therapy for Menopause on the Mechanical Properties of Skin. J Am Geriat, 43:662–5, 1995.

    CAS  Google Scholar 

  7. Pierard-Franchimont C, Letawe C, Goffin V, and Pierard GE: Skin Water-Holding Capacity and Transdermal Estrogen Therapy for Menopause. A Pilot Study. Maturitas, 22:151–4, 1995.

    Article  PubMed  CAS  Google Scholar 

  8. Castelo-Branco C, Figueras F, Martinez de Osaba M, and Vanrell J: Facial Wrinkling in Postmenopausal Women. Effects of Smoking Status and Hormone Replacement Therapy. Maturitas, 20:75–86, 1998.

    Article  Google Scholar 

  9. Pierard-Franchimont C, Cornil F, Dehavay J, Deleixhe-Mauhin F, Letot B, and Pierard G: Climacteric Skin Ageing of the Face—A Prospective Longitudinal Comparative Trial on the Effect of Oral Hormone Replacement Therapy. Maturitas, 32:87–93, 1999.

    Article  PubMed  CAS  Google Scholar 

  10. Bullard K, Cass D, Banda M, and Adzick N: Transforming Growth Factor beta-1 Decreases Interstitial Collagenase in Healing Human Fetal Skin. J Pediatric Surg, 32:1023–7, 1998.

    Article  Google Scholar 

  11. Im, S, Lee, E, Kim, W, Song, J, Kim, J, Lee, M and Kang, W: Expression of Progersterone Receptor in Human Keratinocytes. J Korean Med Sci, 15:647–54, 2000.

    PubMed  CAS  Google Scholar 

  12. Ikeda T, Shigeno C, Kasai R, Kohno H, Ohta S, Okumura H, Konishi J, and Yamamuro T: Ovariectomy Decreases the mRNA Levels of Transforming Growth Factor-Beta I and Increases the mRNA levels of Osteocalcin in Rat Bone in Vivo. Biochem Biophys Res Commun, 194:1228–33, 1993.

    Article  PubMed  CAS  Google Scholar 

  13. Tranquilli A, Mazzanti L, Cugini A, Cester N, Garzetti G, and Romanini C: Transdermal Estradiol and Medroxyprogesterone Acetate in Hormone Replacement Therapy are Both Antioxidants. Gynecol Endocrinol, 9: 137–41, 1995.

    Article  PubMed  CAS  Google Scholar 

  14. Leal M, Diaz J, Serrano E, Abellan J, and Carbonell L: Hormone Replacement Therapy for Oxidative Stress in Postmenopausal Women with Hot Flushes. Obstet Gynecol, 95:804–9, 2000.

    Article  PubMed  CAS  Google Scholar 

  15. Ayres S, Abplanalp W, Liu J, and Subbiah M: Mechanisms Involved in the Protective effect of Estradiol-17 Beta on lipid Peroxidation and DNA damage. Am J Physiol, 274:E1002–8, 1998.

    Google Scholar 

  16. Zeng G, McCue H, Mastrangelo L, and Millis A: Endogenous TGF-β Activity is Modified During Cellular Aging: Effects on Metalloproteinase and TIMP-I Expression. Expt Cell Res, 228:271–276, 1996.

    Article  CAS  Google Scholar 

  17. Bruner K, Eisenberg E, Gorstein F, Osteen K: Progesterone and transforming growth factor-β coordinately regulate suppression of endometrial matrix metalloproteinases in a model of experimental endometriosis, Steroids 64:648–653, 1999.

    Article  PubMed  CAS  Google Scholar 

  18. Singer C, Marbaix E, Kokorine I, Lemoine P, Donnez J, Eeckhout Y, Courtoy P: Paracrine stimulation of interstitial collagenase (MMP-1) in the human endometrium by interleukin 1a and its dual block by ovarian steroids. Proc Natl Acad Sci, 94:10341–10345, 1997.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Biology and Chemistry/Biochemistry, Georgian Court College, 900 Lakewood Avenue, Lakewood, NJ, 08701, USA

    Neena Philips Ph.D. & Jan Devaney

Authors
  1. Neena Philips Ph.D.
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jan Devaney
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Neena Philips Ph.D..

About this article

Cite this article

Philips, N., Devaney, J. Beneficial regulation of type I collagen and matrixmetalloproteinase-1 expression by estrogen, progesterone, and its combination in skin fibroblasts.. AGE 26, 59–62 (2003). https://doi.org/10.1007/s11357-003-0006-7

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11357-003-0006-7

Keywords

Search

Navigation