Abstract
Chemical oxidation as a pretreatment step coupled with bioremediation for petroleum-contaminated soil may pose serious impacts on indigenous microorganisms and the available nutrients. Petroleum-contaminated soil were treated by hydrogen peroxide (H2O2) at initial concentrations of 105 mM (HH), 21 mM (HL), and 105 mM in three equal amounts (HT) without adding any external catalyst. The contents of total petroleum hydrocarbons (TPH) and dissolved nutrients (total organic compounds, nitrogen, and phosphate), and the indigenous bacteria community succession (analyzed by high-throughput sequencing of 16S rDNA) were investigated over 50 days. Compared to the control treatment without H2O2 addition, H2O2 treatments for the petroleum-contaminated soil significantly promoted the TPH removal especially in the first 4 days and impacted the contents of dissolved nutrients. Both of chemical oxidation and nutrients contributed to microbial community structure changes in alpha diversity. Although the soil microbial community structure had undergone significant changes after different chemical oxidation pretreatments, Firmicutes, Proteobacteria, Gemmatimonadetes, and Actinobacteria were the main bacterial phyla. Compared with adding H2O2 at one time, H2O2 added in stepwise was beneficial to indigenous bacterial diversity recovery and TPH removal. H2O2 oxidation treatments showed a great influence on the microbial community structures in the start-up stage, while recovery time rather than the oxidation treatments presented greater effects on the composition of the microbial community structure with the incubation time extended. Therefore, adding H2O2 as pretreatment for petroleum-contaminated soil showed little effect on the structure of soil indigenous microbial community from a long-term scale, and was conducive to the continuous removal of TPH by indigenous microorganisms.
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The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
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Funding
This research was partially supported by the National Natural Science Foundation of China (21707111), the Opening Project of Oil & Gas Field Applied Chemistry Key Laboratory of Sichuan Province (YQKF202119), Sichuan Science and Technology Support Project (2020JDTD0018), Qilian Mountains Eco-environment Research Center in Gansu Province (QLS202004), and China Postdoctoral Science Foundation (2021M692263).
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All authors contributed to the study conception and design. Bing Yang: conceptualization, methodology, writing—review and editing. Mi Zhou, Yuan Meng, Keming Chen, and Xiangfu Huang: material preparation, data collection and analysis, writing—original draft and editing. Jie Xu: validation, writing—review and editing. Yucheng Liu: validation, supervision. Lingli Li and Lili Ma: software, validation, writing—review. Mingyan Chen: methodology, supervision. All authors read and approved the final manuscript.
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Yang, B., Zhou, M., Meng, Y. et al. Hydrocarbons removal and microbial community succession in petroleum-contaminated soil under hydrogen peroxide treatment. Environ Sci Pollut Res 30, 27081–27091 (2023). https://doi.org/10.1007/s11356-022-23875-z
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DOI: https://doi.org/10.1007/s11356-022-23875-z