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Photosynthetic regulation in response to strontium stress in moss Racomitrium japonicum L

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Abstract

Strontium (Sr2+) pollution and its biological effects are of great concern including photosynthetic regulation, which is fundamental to environmental responses, especially for bryophytes during their terrestrial adaptation. Alternative electron flows mediated by flavodiiron proteins (FLVs) and cyclic electron flow (CEF) in photosystem I (PSI) are crucial to abiotic stresses moss responses; however, little is known about the moss photosynthesis regulation under nuclide treatment. We measured chlorophyll fluorescence parameters in PSI, photosystem II (PSII) and the P700 redox state, oxidative stress in the moss Racomitrium japonicum under low (5 mg/L), moderate (50 mg/L) and high (500 mg/L) Sr2+ stress level. Moderate and high Sr2+ stress triggered H2O2 and malondialdehyde (MDA) generation, and catalase (CAT) activity increases, which are involved in reactive oxygen species regulation. The significant PSII photochemistry (Fv/Fm), Chla/chlb, Y(I)/Y(II), Y(NA), Y(ND) and ETRI-ETRII decreases at moderate and high Sr2+, and the Y(I), Y(II) decreases at high Sr2+ revealed the photo-inhibition and photo-damage in PSI and PSII by moderate and high Sr2+ stress. The nonphotochemical quenching (NPQ) increased significantly at moderate and high Sr2+ stress, reflecting a heat-dissipation-related photo-protective mechanism in antenna system and reaction centers. Moreover, rapid re-oxidation of P700 indicated that FLV-dependent flows significantly regulated PSI redox state under moderate and high Sr2+ stress. and CEF upregulation was found at low Sr2+. Finally, photosynthetic acclimation to Sr2+ stress in R. japonicum was linked to FLVs and CEF adjustments.

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Data availability

The datasets analyzed during this study are available from the corresponding author.

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Funding

This work was supported by the National Key Research and Development Program of China (2020YFE0203200), the Open Fund Program of the CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization and Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences (KXYSWS2204), Sichuan Science and Technology Department Program (2020YFS0344, 2022YFS0492, 2021YFH0008, 2022YFH0040) and the Opening Project of Hubei Engineering Research Center for Specialty Flowers Biological Breeding (2022ZD003).

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  1. Hui Ren and Renhua Huang contributed equally to this work.

Authors and Affiliations

  1. School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, 621010, China

    Hui Ren, Wanting Li, Liuliu Zheng & Ke Chen

  2. Hubei Engineering Research Center for Specialty Flowers Biological Breeding, Jingchu University of Technology, Jingmen, 448000, Hubei, China

    Renhua Huang

  3. China-Croatia “Belt and Road” Joint Laboratory On Biodiversity and Ecosystem Services, CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China

    Yanbao Lei

  4. Administration Bureau of Jiuzhaigou National Nature Reserve, Jiuzhaigou, 623402, China

    Ying Li

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  1. Hui Ren
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Contributions

All authors contributed to the study conception and design. KC, RHH and YBL conceived and designed research. HR and YHL conducted experiments. RHH, WTL, LLZ and HR analyzed data. HR, KC and YBL wrote the first draft of the manuscript and intensively edited by all authors.

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Correspondence to Ke Chen.

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Ren, H., Huang, R., Li, Y. et al. Photosynthetic regulation in response to strontium stress in moss Racomitrium japonicum L. Environ Sci Pollut Res 30, 20923–20933 (2023). https://doi.org/10.1007/s11356-022-23684-4

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