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Development of a horseradish peroxidase-Fenton-like system for the degradation of sulfamethazine under weak acid condition

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Abstract

Both horseradish peroxidase (HRP) and ferrous ion (Fe2+) can degrade organic micropollutants (e.g., sulfamethazine) in the presence of hydrogen peroxide (H2O2), but they have their own disadvantages, such as low degradation efficiency and low pH condition, respectively. In order to overcome the above shortcomings, this study is to develop a HRP-Fenton-like system for sulfamethazine (SMR) efficient degradation by analyzing the optimal reaction conditions, degradation mechanisms, and effect of ions. Results show that HRP and Fe2+ achieve effective coupling by adding trace Fe2+ (≤ 10 μmol/mL) to the HRP system at pH = 5, and the degradation rate of SMR increased by 20.7–42% depending on Fe2+ concentration compared with single HRP treatment. Consumption of H2O2 and quenching of hydroxyl radicals confirmed that HRP dominated SMR removal in the HRP-Fenton-like system. HPLC-MS analysis shows that SMR was degraded by C-S bond breaking, N-S bond breaking, hydroxyl substitution, and rearrangement. Furthermore, Cl−, HCO3−, and NO3− exhibit an acceptable negative effect, while SO42− shows a positive effect on the degradation of SMR.

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Data availability

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Funding

This study has been supported by the National Natural Science Foundation of China (42077391) and Shandong Provincial Natural Science Foundation, China (ZR2019BB049).

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Author notes

  1. Hong Liu and Zaihui Huang contributed equally to this work.

Authors and Affiliations

  1. School of Environmental Science and Engineering, Shandong Provincial Key Laboratory of Water Pollution Control and Resource Reuse, China–America CRC for Environment & Health of Shandong Province, Shandong University, 72# Jimo Binhai Road, Qingdao, Shandong, 266237, People’s Republic of China

    Hong Liu, Zaihui Huang & Chunguang Liu

  2. Engineering Research Center of Ministry of Education on Groundwater Pollution Control and Remediation, College of Water Sciences, Beijing Normal University, 19# Xinjiekouwai Street, Beijing, 100875, China

    Hong Liu

  3. Guangzhou Key Laboratory of Environmental Exposure and Health, School of Environment, Jinan University, Guangzhou, China

    Chunguang Liu

Authors
  1. Hong Liu
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  2. Zaihui Huang
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  3. Chunguang Liu
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Contributions

Hong Liu: writing—original draft, data curation, investigation, methodology; Zaihui Huang: investigation, writing—original draft, formal analysis; Chunguang Liu: experiment design, data analysis, and writing.

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Correspondence to Chunguang Liu.

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Responsible Editor: Ricardo A. Torres-Palma

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Highlights

1. HRP-Fenton-like system is developed to degrade SMR by adding Fe2+ to the HRP system.

2. Trace Fe2+ (≤ 10 μM/mL) addition can increased SMR removal by 20.7–42% at pH 5.

3. HRP, not Fenton, dominated SMR removal in the HRP-Fenton-like system.

4. Cl−/HCO3− inhibits Fenton, but has little effect on HRP-Fenton-like for SMR removal.

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Liu, H., Huang, Z. & Liu, C. Development of a horseradish peroxidase-Fenton-like system for the degradation of sulfamethazine under weak acid condition. Environ Sci Pollut Res 29, 12065–12074 (2022). https://doi.org/10.1007/s11356-021-16681-6

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  • DOI: https://doi.org/10.1007/s11356-021-16681-6

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