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Effect of different doses of the catecholamine epinephrine on antioxidant responses of larvae of the flesh fly Sarcophaga dux

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Abstract

The production and use of pharmaceutical products have increased over the past decades, and several are considered potential or proved hazardous wastes. When contaminating the environment, they can severely impact biodiversity. The catecholamine epinephrine (adrenaline) is no exception. Epinephrine can be administered as growth promoter in cattle, and is used for anaphylaxis treatment in human. While a range of studies has examined the effects of this catecholamine on vertebrate tissues, and evidenced that it can disrupt the oxidative stress status, the effects epinephrine could have on insects have remained poorly considered. Here, we examined the physiological effects of different concentrations (0, 25, 50, and 100 μg/mL) of epinephrine on larvae of the flesh fly Sarcophaga dux. Following experimental treatments, levels of H2O2, GSH, CAT, GPx, and CEH were measured from the fat body, cuticle, gut, and hemolymph of 3rd instars. Significant differences are reported for these physiological endpoints among the considered body compartments, and epinephrine concentrations. Epinephrine treatments did not increase reactive oxygen species production (H2O2 amounts), except for gut tissues. Increased levels of GSH suggest that epinephrine may have enhanced glucose metabolism and flux towards the pentose phosphate pathway, while reducing glutamine oxidation. CAT activity was slightly increased when the concentration of epinephrine was higher. The decreased GPx activity in the fat body was consistent with GSH variations. In sum, the injection of epinephrine seemed to elicit the antioxidant response in S. dux larvae, in turn attenuating ROS production.

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Data Availability

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

This study was done at the Entomology Department, Faculty of Science, Cairo University, Egypt.

Funding

This study was funded by the Entomology Department (Faculty of Science, Cairo University, Egypt).

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Authors and Affiliations

  1. Entomology Department, Faculty of Science, Cairo University, 11221 Al Orman, Giza, Egypt

    Eman A. Abdelfattah

  2. University of Rennes, CNRS, ECOBIO [(Ecosystèmes, biodiversité, évolution)] - UMR 6553, 263 Avenue du Gal Leclerc, 74205, F 35000, 35042, Rennes, CS, France

    David Renault

  3. Institut Universitaire de France, 1 rue Descartes, 75231, Paris Cedex 05, France

    David Renault

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  1. Eman A. Abdelfattah
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Contributions

Eman A. Abdelfattah: conceptualization, investigation, methodology, resources, writing (original draft preparation), visualization, and funding acquisition

David Renault: conceptualization, supervision, writing (original draft preparation), writing (review and editing), and visualization

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Correspondence to Eman A. Abdelfattah or David Renault.

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Highlights

• The effects of different doses of epinephrine on flesh fly larvae were examined.

• Levels of H2O2, GSH, CAT, GPx, and CEH were measured from different body parts.

• Increased levels of GSH were found in epinephrine-treated larvae.

• CAT activity was slightly increased when the concentration of epinephrine was higher.

• Epinephrine can reduce the production of reactive oxygen species.

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Abdelfattah, .A., Renault, D. Effect of different doses of the catecholamine epinephrine on antioxidant responses of larvae of the flesh fly Sarcophaga dux. Environ Sci Pollut Res 29, 10408–10415 (2022). https://doi.org/10.1007/s11356-021-16325-9

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