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Study on the mechanism of Salvia miltiorrhiza polysaccharides in relieving liver injury of broilers induced by florfenicol

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Abstract

In order to explore the transcriptomics and proteomics targets and pathways of Salvia miltiorrhiza polysaccharides (SMPs) alleviating florfenicol (FFC)-induced liver injury in broilers, 60 1-day-old broilers were randomly divided into 3 groups: control group ( GP1) was fed tap water, FFC model (GP2) was given tap water containing FFC 0.15 g/L, and SMPs treatment group (GP3) was given tap water containing FFC 0.15 g/L and SMPs 5 g/L. Starting from 1 day of age, the drug was administered continuously for 5 days. On the 6th day, blood was collected from the heart and the liver was taken. Then 3 chickens were randomly taken from each group, and their liver tissues were aseptically removed and placed in an enzyme-free tube. Using high-throughput mRNA sequencing and TMT-labeled quantitative proteomics technology, the transcriptome and proteome of the three groups of broiler liver were analyzed, respectively. The results of the study showed that the liver tissue morphology of the chicks in the GP1 and GP3 groups was complete and there were no obvious necrotic cells in the liver cells. The liver tissue cells in the GP2 group showed obvious damage, the intercellular space increased, and the liver cells showed extensive vacuolation and steatosis. Compared with the GP1 group, the daily gain of chicks in the GP2 group was significantly reduced (P < 0.0 5 or P < 0.01). Compared with the GP2 group, the GP3 group significantly increased the daily gain of chicks (P <0.0 5 or P <0.01). Compared with the GP1 group, the serum levels of ALT, AST, liver LPO, ROS, and IL-6 in the GP2 group were significantly increased (P < 0.0 5 or P < 0.01), and the contents of T-AOC, GSH-PX, IL-4, and IL-10 in the liver were significantly decreased (P < 0.0 5 or P < 0.01). After SMPs treatment, the serum levels of ALT, AST, liver LPO, ROS, and IL-6 were significantly reduced (P < 0.0 5 or P < 0.01), and the contents of T-AOC, GSH-PX, IL-4, and IL-10 in the liver were significantly increased (P < 0.0 5 or P < 0.01). There were 380 mRNA and 178 protein differentially expressed between GP2 group and GP3 group. Part of DEGs was randomly selected for QPCR verification, and the expression results of randomly selected FABP1, SLC16A1, GPT2, AACS, and other genes were verified by QPCR to be consistent with the sequencing results, which demonstrated the accuracy of transcriptation-associated proteomics sequencing. The results showed that SMPs could alleviate the oxidative stress and inflammatory damage caused by FFC in the liver of chicken and restore the normal function of the liver. SMPs may alleviate the liver damage caused by FFC by regulating the drug metabolism-cytochrome P450, PPAR signaling pathway, MAPK signaling pathway, glutathione metabolism, and other pathways.

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Data availability

The datasets used and analyzed during the current study are available from the corresponding author on reasonable request.

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Funding

The authors are thankful to the Natural Science Foundation of Hebei Province, China——Study on the Mechanism of Salvia Miltiorrhiza Polysaccharides Alleviating Florfenicol-induced Liver Injury in Broilers Based on Multi-omics Integration Analysis (C2021204026) and the Precision Animal Husbandry Discipline Group Construction Project of Hebei Agricultural University (1090064) for the support in this work.

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Authors and Affiliations

  1. College of Traditional Chinese Veterinary Medicine, Hebei Agricultural University, 2596, Le Kai South Street, Baoding, 071001, China

    Yumeng Geng, Chunyu Lu, Guozhong Jin, Shuying Li, Yuqing Cui, Chao Han, Wanyu Shi & Yongzhan Bao

  2. Hebei Veterinary Biotechnology Innovation Center, Baoding, 071000, China

    Wanyu Shi & Yongzhan Bao

Authors
  1. Yumeng Geng
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  2. Chunyu Lu
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  3. Guozhong Jin
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  4. Shuying Li
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  5. Yuqing Cui
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  6. Chao Han
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  7. Wanyu Shi
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  8. Yongzhan Bao
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Contributions

Wanyu Shi and Yongzhan Bao conceived and designed the study, and they rigorously revised the manuscript. Yumeng Geng performed most of the experiments and analyzed the results and drafted the manuscript. Chunyu Lu and Guozhong Jin helped collect samples and participated in the H&E. Shuying Li and Yuqing Cui participated in the qPCR experiment and analyzed the results. Chao Han participated in the detection and result analysis of biochemical indicators. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Wanyu Shi.

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The use of animals in this study was approved by the Hebei Animal Protection Association.

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Informed consent was obtained from all individual participants included in the study.

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Consent was obtained from participants about publishing their data.

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The authors declare no competing interests.

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Responsible Editor: Mohamed M. Abdel-Daim

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Geng, ., Lu, C., Jin, G. et al. Study on the mechanism of Salvia miltiorrhiza polysaccharides in relieving liver injury of broilers induced by florfenicol. Environ Sci Pollut Res 29, 3372–3385 (2022). https://doi.org/10.1007/s11356-021-15687-4

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  • DOI: https://doi.org/10.1007/s11356-021-15687-4

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