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DNA methylation of JAK3/STAT5/PPARγ regulated the changes of lipid levels induced by di (2-ethylhexyl) phthalate and high-fat diet in adolescent rats

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Abstract

Di (2-ethylhexyl) phthalate (DEHP) and high-fat diet (HFD) could induce lipid metabolic disorder. This study was undertaken to identify the effect of DNA methylation of JAK3/STAT5/PPARγ on lipid metabolic disorder induced by DEHP and HFD. Wistar rats were divided into a normal diet (ND) group and HFD group. Each diet group treated with DEHP (0, 5, 50, 500 mg/kg/d) for 8 weeks’ gavage. The DNA-methylated levels of PPARγ, JAK3, STAT5a, and STAT5b in rats’ livers and adipose were analyzed with MethylTarget. The lipid levels of rats’ livers and adipose were detected with ELISA. Results showed in ND group that the DNA methylation levels of PPARγ, JAK3 in livers, and STAT5b in adipose were lower in 500 mg/kg/d group than the control. And the level of total cholesterol (TC) in adipose was higher in 500 mg/kg/d group than the control. In HFD group, the DNA methylation level of JAK3 was the lowest in livers and the highest in adipose in 50 mg/kg/d group. And the level of TC in livers was the lowest in 50 mg/kg/d group. In the 500 mg/kg/d group, the DNA methylation level of STAT5b was lower in livers and higher in adipose in HFD group than that in ND group. And the levels of TC in livers were lower in HFD group than those in ND group. Therefore, DNA methylation of JAK3/STAT5/PPARγ regulated the changes in lipid levels induced by DEHP and HFD in adolescent rats.

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Funding

This study was supported by the National Natural Science Foundation of China (Grant No.81972996, 81573184), and the Plan B of the Bethune Medical Department of Jilin University (2018B12).

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Authors and Affiliations

  1. Department of Occupational and Environmental Health, School of Public Health, Jilin University, 1163 Xin Min Street, Changchun, 130021, China

    Qi Xu, Wen Qi, Yuezhu Zhang, Qi Wang, Shuang Ding, Xu Han, Yaming Zhao, Xinyue Song, Tianyang Zhao, Liting Zhou & Lin Ye

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  1. Qi Xu
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  2. Wen Qi
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  10. Liting Zhou
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  11. Lin Ye
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Contributions

LY and LTZ designed the experiments; QX, YZZ, XH, and YMZ conducted the experiments; QX, SD, XYS, and TYZ analyzed the data; QX wrote the manuscript. All authors reviewed the manuscript.

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Correspondence to Liting Zhou or Lin Ye.

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Xu, Q., Qi, W., Zhang, Y. et al. DNA methylation of JAK3/STAT5/PPARγ regulated the changes of lipid levels induced by di (2-ethylhexyl) phthalate and high-fat diet in adolescent rats. Environ Sci Pollut Res 27, 30232–30242 (2020). https://doi.org/10.1007/s11356-020-08976-x

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