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Role of soluble and exchangeable nitrogen pools in N cycling and the impact of nitrogen added in forest soil

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Abstract

Nitrogen (N) cycle in forest soils is altered by water, salt, or acid solutions, and its internal transfers to and from each existing inorganic pools are not known comprehensively. To evaluate the soluble and exchangeable N pools, bulk soil (B soil), water-extracted soil (W soil), and the 0.5 mol L−1 K2SO4–treated soil (K soil) were incubated for up to 48 days to comprehend the dynamics of inorganic (NH4+ and NO3) and soluble organic N (SON) in water-soluble, exchangeable, 2.5 mol L−1 H2SO4 (labile pool I, LPI) and 13 mol L−1 H2SO4 (labile pool II, LPII) pools. To test the N deposition effects, additional NH4NO3 solution was added to B, W, and K soils at amount of 40 mg N kg−1 soil. The results showed that though there was more NO3 removed when W soil was prepared, the similar net nitrification rate in W soil to B soil and more than 20 mg N kg−1 water-soluble NO3 were observed in W soil, which indicated that the loss of NO3 would be enhanced. In contrast, there was more water-soluble and exchangeable NH4+ for K soil compared with B soil. The different dynamic of NO3 between W and K soil suggested that nitrifiers might dominate in the soil matrix rather than the soil solution. After incubation, each N form in the LPI decreased, which can be attributed to the allocation of remaining N into the recalcitrant pool, except the increase of NH4+ for B soil and NO3 for K soil, and NO3 in LPII for B soil. Compared with control, N addition increased mineralization of exchangeable SON to promote nitrification regardless of soils, but weakened the immobilization of NO3. In addition, N in LPI and LPII pools have increased, which might be related to decomposition of recalcitrant organic matter induced by N addition to transform when the water-soluble and exchangeable N was removed. Therefore, the changes of soluble and exchangeable nitrogen pools impact the N cycling. Our findings can give some explanation for whole soil N transformation responses to N deposition.

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References

  • Beuters P, Scherer HW, Spott O, Vetterlein D (2015) Impact of potassium on plant uptake of non-exchangeable NH4+-N. Plant Soil 387:37–47

    Article  CAS  Google Scholar 

  • Brzostek ER, Greco A, Drake JE, Finzi AC (2013) Root carbon inputs to the rhizosphere stimulate extracellular enzyme activity and increase nitrogen availability in temperate forest soils. Biogeochemistry 115:65–76

    Article  CAS  Google Scholar 

  • Cavalli D, Consolati G, Marino P, Bechini L (2015) Measurement and simulation of soluble, exchangeable, and non-exchangeable ammonium in three soils. Geoderma 259–260:116–125

    Article  Google Scholar 

  • Che J, Zhao XQ, Zhou X, Jia ZJ, Shen RF (2015) High pH-enhanced soil nitrification was associated with ammonia-oxidizing bacteria rather than archaea in acidic soils. Appl Soil Ecol 85:21–29

    Article  Google Scholar 

  • Chen H, Li DJ, Zhao J, Xiao KC, Wang KL (2018) Effects of nitrogen addition on activities of soil nitrogen acquisition enzymes: a meta-analysis. Agric Ecosyst Environ 252:126–131

    Article  CAS  Google Scholar 

  • Chen CR, Xu ZH (2006) On the nature and ecological functions of soil soluble organic nitrogen (SON) in forest ecosystems. J Soils Sediments 6:63–66

    Article  Google Scholar 

  • Chen CR, Xu ZH, Zhang SL, Keay P (2005) Soluble organic nitrogen pools in forest soils of subtropical Australia. Plant Soil 277:285–297

    Article  CAS  Google Scholar 

  • Chen XY, Wu LH, Cao XC, Zhu YH (2013) Organic nitrogen components in soils from southeast China. J Zhejiang Univ Sci B (Biomedicine and Biotechnology) 14:259–269

    Article  CAS  Google Scholar 

  • Cheng Y, Wang J, Chang SX, Cai Z, Müller C, Zhang J (2019) Nitrogen deposition affects both net and gross soil nitrogen transformations in forest ecosystems: a review. Environ Pollut 244:608–616

    Article  CAS  Google Scholar 

  • Cookson WR, Murphy DV (2004) Quantifying the contribution of dissolved organic matter to soil nitrogen cycling using 15N isotopic pool dilution. Soil Biol Biochem 36:2097–2100

    Article  CAS  Google Scholar 

  • Currey PM, Johnson D, Sheppard LJ, Leith ID, Toberman H, Wal RV, Dawson LA, Artz RRE (2010) Turnover of labile and recalcitrant soil carbon differ in response to nitrate and ammonium deposition in an ombrotrophic peatland. Glob Chang Biol 16:2307–2321

    Article  Google Scholar 

  • Dou X, Li F, Cheng X, Zhu P (2018) Soil organic carbon and nitrogen dynamics induced by continuous maize cropping compared to maize–soya bean rotation. Eur J Soil Sci 69:535–544

    Article  CAS  Google Scholar 

  • Frey SD, Ollinger S, Nadelhoffer K, Bowden R, Brzostek E, Burton A, Caldwell BA, Crow S, Goodale CL, Grandy AS, Finzi A, Kramer MG, Lajtha K, LeMoine J, Martin M, McDowell WH, Minocha R, Sadowsky JJ, Templer PH, Wickings K (2014) Chronic nitrogen additions suppress decomposition and sequester soil carbon in temperate forests. Biogeochemistry 121:305–316

    Article  CAS  Google Scholar 

  • Gärdenäs AI, Ågren GI, Bird JA, Clarholm M, Hallin S, Ineson P, Kätterer T, Knicker H, Nilsson SI, Näsholm T, Ogle S, Paustian K, Persson T, Stendahl J (2011) Knowledge gaps in soil carbon and nitrogen interactions e From molecular to global scale. Soil Biol Biochem 43:702–717

    Article  Google Scholar 

  • Gao J, Jansen B, Cerli C, Helmus R, Mikutta R, Dultz S, Guggenberger G, Vogel C, Kalbitz K (2018) Organic matter coatings of soil minerals affect adsorptive interactions with phenolic and amino acids. Eur J Soil Sci 69:613–624

    Article  CAS  Google Scholar 

  • Gao W, Kou L, Zhang J, Müller C, Wang H, Yang H, Li S (2016) Enhanced deposition of nitrate alters microbial cycling of N in a subtropical forest soil. Biol Fertil Soils 52:977–986

    Article  CAS  Google Scholar 

  • Gao W, Yang H, Kou L, Li SG (2015) Effects of nitrogen deposition and fertilization on N transformations in forest soils: a review. J Soils Sediments 15:863–879

    Article  CAS  Google Scholar 

  • Haynes RJ (2000) Labile organic matter as an indicator of organic matter quality in arable and pastoral soils in New Zealand. Soil Biol Biochem 32:211–219

    Article  CAS  Google Scholar 

  • Jiang YM, Chen CR, Liu YQ, Xu ZH (2010) Soil soluble organic carbon and nitrogen pools under mono- and mixed species forest ecosystems in subtropical China. J Soils Sediments 10:1071–1081

    Article  CAS  Google Scholar 

  • Jiao Y, Xu Z, Zhao JH, Yang WZ (2012) Changes in soil carbon stocks and related soil properties along a 50-year grassland-to-cropland conversion chronosequence in an agro-pastoral ecotone of Inner Mongolia, China. J Arid Land 4:420–430

    Article  Google Scholar 

  • Liu XJ, Duan L, Mo JM, Du EZ, Shen JL, Lu XK, Zhang Y, Zhou XB, He CE, Zhang FS (2011) Nitrogen deposition and its ecological impact in China: an overview. Environ Pollut 159:2251–2264

    Article  CAS  Google Scholar 

  • Lu M, Yang Y, Luo Y, Fang C, Zhou X, Chen J, Yang X, Li B (2011) Responses of ecosystem nitrogen cycle to nitrogen addition: a meta-analysis. New Phytol 189:1040–1050

    Article  CAS  Google Scholar 

  • Luce MS, Whalen JK, Noura Z, Zebarth BJ, Chantigny MH (2014) Labile organic nitrogen transformations in clay and sandy-loam soils amended with 15N-labelled faba bean and wheat residues. Soil Biol Biochem 68:208–218

    Article  Google Scholar 

  • Luo X, Hou E, Zhang L, Zang X, Yi Y, Zhang G, Wen D (2019) Effects of forest conversion on carbon-degrading enzyme activities in subtropical China. Sci Total Environ 696:133968

    Article  CAS  Google Scholar 

  • McFarland JW, Ruess RW, Kielland K, Doyle AP (2002) Cycling dynamics of NH4+ and amino acid nitrogen in soils of a deciduous boreal forest ecosystem. Ecosystems 5:775–788

    CAS  Google Scholar 

  • Muqaddas B, Lewis T, Esfandbod M, Chen C (2019) Responses of labile soil organic carbon and nitrogen pools to long-term prescribed burning regimes in a wet sclerophyll forest of southeast Queensland, Australia. Sci Total Environ 647:110–120

    Article  CAS  Google Scholar 

  • Nieder R, Benbi DK, Scherer HW (2011) Fixation and defixation of ammonium in soils: a review. Biol Fertil Soils 47:1–14

    Article  CAS  Google Scholar 

  • Prendergast-Miller MT, de Menezes AB, Farrell M, Macdonald LM, Richardson AE, Bissett A, Toscas P, Baker G, Wark T, Thrall PH (2015) Soil nitrogen pools and turnover in native woodland and managed pasture soils. Soil Biol Biochem 85:63–71

    Article  CAS  Google Scholar 

  • Quan Z, Huang B, Lu C, Shi Y, Chen X, Zhou J, Fang Y (2018) Formation of extractable organic nitrogen in an agricultural soil: a 15N labeling study. Soil Biol Biochem 118:161–165

    Article  CAS  Google Scholar 

  • Ros GH, Hoffland E, Temminghoff EJM (2010) Dynamics of dissolved and extractable organic nitrogen upon soil amendment with crop residues. Soil Biol Biochem 42:2094–2101

    Article  CAS  Google Scholar 

  • Rovira P, Vallejo VR (2002) Labile and recalcitrant pools of carbon and nitrogen in organic matter decomposing at different depths in soil: an acid hydrolysis approach. Geoderma 107:109–141

    Article  CAS  Google Scholar 

  • Rovira P, Vallejo VR (2007) Labile, recalcitrant, and inert organic matter in Mediterranean forest soils. Soil Biol Biochem 39:202–215

    Article  CAS  Google Scholar 

  • Scherer HW, Feils E, Beuters P (2014) Ammonium fixation and release by clay minerals as influenced by potassium. Plant Soil Environ 60:325–331

    Article  CAS  Google Scholar 

  • Schimel JP, Bennett J (2004) Nitrogen mineralisation: challenges of a changing paradigm. Ecology 85:591–602

    Article  Google Scholar 

  • Soil Survey Staff (2014) Keys to Soil Taxonomy. 12th ed. Washington: USDA; Natural Resources Conservation Service.

  • Toosi ER, Clinton PW, Beare MH, Norton DA (2012) Biodegradation of soluble organic matter as affected by land-use and soil depth. Soil Sci Soc Am J 76:1667–1677

    Article  CAS  Google Scholar 

  • Warren CR (2014) Organic N molecules in the soil solution: what is known, what is unknown and the path forwards. Plant Soil 375:1–19

    Article  CAS  Google Scholar 

  • Xing S, Chen C, Zhou B, Zhang H, Nang ZM, Xu ZH (2010) Soil soluble organic nitrogen and active microbial characteristics under adjacent coniferous and broadleaf plantation forests. J Soils Sediments 10:748–757

    Article  CAS  Google Scholar 

  • Yang K, Zhu J, Yan Q, Zhang J (2012) Soil enzyme activities as potential indicators of soluble organic nitrogen pools in forest ecosystems of Northeast China. Ann For Sci 69:795–803

    Article  Google Scholar 

  • Zhang W, Liang C, Kao-Kniffin J, He HB, Xie HT, Zhang H, Zhang XD (2015) Differentiating the mineralization dynamics of the originally present and newly synthesized amino acids in soil amended with available carbon and nitrogen substrates. Soil Biol Biochem 85:162–169

    Article  CAS  Google Scholar 

  • Zhang Y, Tigabu M, Yi Z, Li H, Zhuang Z, Yang Z, Ma X (2019) Soil parent material and stand development stage effects on labile soil C and N pools in Chinese fir plantations. Geoderma 338:247–258

    Article  CAS  Google Scholar 

Download references

Funding

This work is supported by the National Natural Science Foundation of China (Grant No. 31770659, 31570607 and 31470628) and by the Natural Science Foundation of Fujian Province (2018J01716).

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Correspondence to Hongliang Ma.

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Ma, H., Tecimen, H.B., Lin, W. et al. Role of soluble and exchangeable nitrogen pools in N cycling and the impact of nitrogen added in forest soil. Environ Sci Pollut Res 27, 5398–5407 (2020). https://doi.org/10.1007/s11356-019-07316-y

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