Abstract
Transporters play an important role in the uptake and redistribution of agrochemicals to the site of insect feeding. The product of the Arabidopsis thaliana gene AtAAP1 substantially contributes to inorganic nitrogen acquisition under ecologically relevant amino acid concentrations. Here, the transporter ability of AtAAP1 to a chlorantraniliprole-alanine conjugate (CAP-Ala-1) was tested both in planta and in vitro. Thirty-day-old and 15-day-old plants overexpressing AtAAP1 increased the uptake of CAP-Ala-1 into the roots, whereas AtAAP1 deficiency did not completely block the uptake of CAP-Ala-1. An uptake experiment carried out in Xenopus laevis oocytes expressing AtAAP1 showed that CAP-Ala-1 interacted with AtAAP1. Although little native AtAAP1 transcription was present in the leaves, constitutive expression of AtAAP1 in plants significantly increased the ability of the leaf mesophyll protoplasts to take up CAP-Ala-1. The observations supported the possibility of exploiting AtAAP1 as a component of a novel delivery and redistribution system for amino acid-based pesticide conjugates.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
An YQ, McDowell JM, Huang S, McKinney EC, Chambliss S, Meagher RB (1996) Strong, constitutive expression of the Arabidopsis ACT2/ACT8 ctin subclass in vegetative tissues. Plant J 10:107-121. https://doi.org/10.1046/j.1365-313x.1996.10010107.x
Chen LS, Bush DR (1997) LHT1, A lysine- and histidine-specific amino acid transporter in Arabidopsis. Plant Physiol 115:1127–1134
Chen Y et al (2018) AtLHT1 transporter can facilitate the uptake and translocation of a glycinergic-chlorantraniliprole conjugate in Arabidopsis thaliana. J Agric Food Chem 66:12527–12535. https://doi.org/10.1021/acs.jafc.8b03591
Damak M, Mahmoudi SR, Hyder MN, Varanasi KK (2016) Enhancing droplet deposition through in-situ precipitation. Nat Commun 7:12560. https://doi.org/10.1038/ncomms12560
Delétage-Grandon C, Chollet JF, Faucher M, Rocher F, Komor E, Bonnemain JL (2001) Carrier-mediated uptake and phloem systemyof a 350-Dalton chlorinated xenobiotic with an alpha-amino acid function. Plant Physiol 125:1620–1632
Denis M, Delrot S (1993) Carrier-mediated uptake of glyphosate in broad bean (Vicia faba) via a phosphate transporter. Physiol Plant 87:569–575
Hirner A, Ladwig F, Stransky H, Okumoto S, Keinath M, Harms A, Frommer WB, Koch W (2006) Arabidopsis LHT1 is a high-affinity transporter for cellular amino acid uptake in both root epidermis and leaf mesophyll. Plant Cell 18:1931–1946. https://doi.org/10.1105/tpc.106.041012
Hu AL, Yang W, Xu HH (2010) Novel fluorescent conjugate containing glucose and NBD and its carrier-mediated uptake by tobacco cells. J Photochem Photobiol B 101:215–223. https://doi.org/10.1016/j.jphotobiol.2010.07.006
Jiang X, Xie Y, Ren Z, Ganeteg U, Lin F, Zhao C, Xu HH (2018) Design of a new glutamine-gipronil conjugate with alpha-amino acid function and its uptake by A. thaliana lysine histidine transporter 1 ( AtLHT1). J Agric Food Chem 66:7597–7605. https://doi.org/10.1021/acs.jafc.8b02287
Lee YH, Foster J, Chen J, Voll LM, Weber AP, Tegeder M (2007) AAP1 transports uncharged amino acids into roots of Arabidopsis. Plant J 50:305–319. https://doi.org/10.1111/j.1365-313X.2007.03045.x
Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)). Methods 25:402–408. https://doi.org/10.1006/meth.2001.1262
Mao GL, Yan Y, Chen Y, Wang BF, Xu FF, Zhang ZX, Lin F, Xu HH (2017) Family of Ricinus communis monosaccharide transporters and RcSTP1 in promoting the uptake of a glucose-fipronil conjugate. J Agric Food Chem 65:6169–6178. https://doi.org/10.1021/acs.jafc.7b02044
Ortiz-Lopez A, Chang H-C, Bush DR (2000) Amino acid transporters in plants. Biochimica et Biophysica Acta 1465:275–280
Perchlik M, Foster J, Tegeder M (2014) Different and overlapping functions of Arabidopsis LHT6 and AAP1 transporters in root amino acid uptake. J Exp Bot65:5193–5204
Schröder P, Scheer CE, Diekmann F, Stampfl A (2007) How plants cope with foreign compounds. Environ Sci Pollut Res Int 14:114–122
Sheng QQ, Liu X, Xie Y, Lin F, Zhang ZX, Zhao C, Xu HH (2018) Synthesis of novel amino acid-fipronil conjugates and study on their phloem loading mechanism. Molecules 23. https://doi.org/10.3390/molecules23040778
Svennerstam H, Ganeteg U, Nasholm T (2008) Root uptake of cationic amino acids by Arabidopsis depends on functional expression of amino acid permease 5. New Phytol 180:620–630. https://doi.org/10.1111/j.1469-8137.2008.02589.x
Svennerstam H, Jamtgard S, Ahmad I, Huss-Danell K, Nasholm T, Ganeteg U (2011) Transporters in Arabidopsis roots mediating uptake of amino acids at naturally occurring concentrations. New Phytol 191:459–467. https://doi.org/10.1111/j.1469-8137.2011.03699.x
Tegeder M (2012) Transporters for amino acids in plant cells: some functions and many unknowns. Curr Opin Plant Biol 15:315–321
Vryzas Z (2016) The Plant as metaorganism and research on next-generation systemic pesticides - prospects and challenges. Front Microbiol 7. https://doi.org/10.3389/fmicb.2016.01968
Wang CJ, Liu ZQ (2007) Foliar uptake of pesticides—present status and future challenge. Pesticide Biochem Physiol 87:1–8. https://doi.org/10.1016/j.pestbp.2006.04.004
Wang T, Chen Y, Zhang M, Chen J, Liu J, Han H, Hua X (2017) Arabidopsis AMINO ACID PERMEASE 1 contributes to salt stress-induced proline uptake from exogenous sources. Front Plant Sci 8:2182. https://doi.org/10.3389/fpls.2017.02182
Wu HX, Yang W, Zhang ZX, Huang T, Yao GK, Xu HH (2012) Uptake and phloem transport of glucose-fipronil conjugate in Ricinus communis involve a carrier-mediated mechanism. J Agric Food Chem 60:6088–6094. https://doi.org/10.1021/jf300546t
Wu H, Xu HH, Marivingt-Mounir C, Bonnemain JL, Chollet JF (2018) Vectorizing agrochemicals: enhancing bioavailability via carrier-mediated transport. Pest Manag Sci. https://doi.org/10.1002/ps.5298
Xie Y, Zhao JL, Wang CW, Yu AX, Liu N, Chen L, Lin F, Xu HH (2016) Glycinergic-fipronil uptake is mediated by an amino acid carrier system and induces the expression of amino acid transporter genes in Ricinus communis seedlings. J Agric Food Chem 64:3810–3818. https://doi.org/10.1021/acs.jafc.5b06042
Yao G, Wen Y, Zhao C, Xu HH (2017) Novel amino acid ester-chlorantraniliprole conjugates: design, synthesis, phloem accumulation and bioactivity. Pest Manag Sci 73:2131–2137. https://doi.org/10.1002/ps.4592
Zhang X, Henriques R, Lin SS, Niu QW, Chua NH (2006) Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method. Nat Protoc 1:641–646. https://doi.org/10.1038/nprot.2006.97
Funding
This research was supported by the National Key R&D Program of China (2017YFD0200307 and 2017YFD0201100), the Project of Science and Technology in Guangdong Province (Grant No. 2018A030313188), and the Scientific Project in Guangzhou City (Grant No. 201707020013 and 201704030027).
Author information
Authors and Affiliations
Corresponding authors
Additional information
Responsible editor: Gangrong Shi
Publisher’s note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Electronic supplementary material
ESM 1
(PDF 126 kb)
Rights and permissions
About this article
Cite this article
Ren, Z., Chen, Z., Luo, X. et al. Overexpression of AtAAP1 increased the uptake of an alanine-chlorantraniliprole conjugate in Arabidopsis thaliana. Environ Sci Pollut Res 26, 36680–36687 (2019). https://doi.org/10.1007/s11356-019-06671-0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11356-019-06671-0