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Efficacy of Origanum vulgare essential oil and carvacrol against the housefly, Musca domestica L. (Diptera: Muscidae)

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Abstract

The toxicity of Origanum vulgare essential oil to the housefly Musca domestica L. was evaluated. The major constituents of the O. vulgare essential oil by gas chromatographic mass spectrometry (GC-MS) analysis were carvacrol (58.13%), p-cymene (17.85%), thymol (8.15%), γ-terpinene (4.96%), and linalool (3.69%). Toxicity of O. vulgare essential oil against larvae and pupae was evaluated using fumigation and contact assays. The contact toxicity (LC50) of O. vulgare essential oil and carvacrol for larvae was 0.23 and 0.03 μL/cm2, respectively. The fumigation toxicity (LC50) of O. vulgare essential oil and carvacrol for larvae was 9.52 and 2.78 μL/L, respectively. Pupal toxicity was evaluated by percentage inhibition rate (PIR). PIR of O. vulgare essential oil at 0.25 μL/cm2 was 90.9% for the contact assay and 100% at 20 μL/L for the fumigation assay. PIR of carvacrol was 29.5% (0.025 μL/cm2) and 81.8% (1.25 μL/L) for the contact toxicity and fumigation assay, respectively. O. vulgare essential oil and carvacrol have significant toxicity to the housefly and are potential insecticides for housefly control.

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References

  • Abdelgaleil SAM, Mohamed MIE, Shawir MS, Abou-Taleb HK (2016) Chemical composition, insecticidal and biochemical effects of essential oils of different plant species from northern Egypt on the rice weevil, Sitophilus oryzae L. J Pest Sci 89:219–229

    Article  Google Scholar 

  • Abou-Taleb HK, Mohamed MIE, Shawir MS, Abdelgaleil SAM (2015) Insecticidal properties of essential oils against Tribolium castaneum (Herbst) and their inhibitory effects on acetylcholinesterase and adenosine triphosphatases. Nat Prod Res 30:710–714

    Article  CAS  Google Scholar 

  • Adams RP (2007) Identification of essential oil components by gascromatography/quadrupole mass spectroscopy, 4th edn. Allured Publishing Corporation, Carol Stream, IL, p 455

    Google Scholar 

  • Anjali CH, Sharma Y, Mukherjee A, Chandrasekaran N (2012) Neem oil (Azadirachta indica) nanoemulsion—a potent larvicidal agent against Culex quinquefasciatus. Pest Manag Sci 68:158–163

    Article  CAS  Google Scholar 

  • Ashraf SA, Al-Shammari E, Hussain T, Tajuddin S, Panda BP (2017) In-vitro antimicrobial activity and identification of bioactive components using GC–MS of commercially available essential oils in Saudi Arabia. J Food Sci Technol 54(12):3948–3958

    Article  CAS  Google Scholar 

  • Benelli G, Pavela R, Giordani C, Casettari L, Curzi G, Cappellacci L, Petrelli R, Maggi F (2018) Acute and sub-lethal toxicity of eight essential oils of commercial interest against the filariasis mosquito Culex quinquefasciatus and the housefly Musca domestica. Ind Crop Prod 112:668–680

    Article  CAS  Google Scholar 

  • Bernath J, Padulosi S (1996) Origanum dictamnus L. and Origanum vulgare L. ssp. Hirtum (link) Letswaart: traditional uses and production in Greece. In: Proceedings of the IPGRI international workshop on oregano. CIHEAM, Bari, pp 8–12

    Google Scholar 

  • Bojar W, Gruszecki TM, Junkuszew A, Dudko P, Greguła-Kania M, Szczepaniak K, Studzińska M, Roczeń-Karczmarz M, Tomczuk K, Le Scouarnec J, Milerski M (2017) Influence of dietary supplementation with a formulation containing essential oils from Origanum vulgare (Lamiacea) and Citrus spp. (Citraceae) as a means of prevention against invasions of gastrointestinal parasites in sheep. Med Weter 73(11):694–697

    Google Scholar 

  • Chauhan N, Malik A, Sharma S, Dhiman RC (2016) Larvicidal potential of essential oils against Musca domestica and Anopheles stephensi. Parasitol Res 115:2223–2231

    Article  Google Scholar 

  • Chauhan N, Malik A, Sharma S (2018) Repellency potential of essential oils against housefly, Musca domestica L. Environ Sci Pollut Res 25:4707–4714

    Article  CAS  Google Scholar 

  • Falco ED, Mancini E, Roscigno G, Mignola E, Taglialatela-Scafati O, Senatore F (2013) Chemical composition and biological activity of essential oils of Origanum vulgare L. subsp. vulgare L. under different growth conditions. Molecules 18:14948–14960

    Article  CAS  Google Scholar 

  • Gallardo A, Picollo MI, Mougabure-Cueto G (2015) Lethal activity of individual and mixed monoterpenoids of geranium essential oil on Musca domestica. Parasitol Res 114:1229–1232

    Article  Google Scholar 

  • Giatropoulos A, Kimbaris A, Michaelakis A, Papachristos DP, Polissiou MG, Emmanouel N (2018) Chemical composition and assessment of larvicidal and repellent capacity of 14 Lamiaceae essential oils against Aedes albopictus. Parasitol Res 117:1953–1964

    Article  Google Scholar 

  • Govindarajan M, Rajeswary M, Hoti SL, Benelli G (2016) Larvicidal potential of carvacrol and terpinen-4-ol from the essential oil of Origanum vulgare (Lamiaceae) against Anopheles stephensi, Anopheles subpictus, Culex quinquefasciatus and Culex tritaeniorhynchus (Diptera: Culicidae). Res Vet Sci 104:77–82

    Article  CAS  Google Scholar 

  • Grevsen K, Fretté XC, Christensen LP (2009) Content and composition of volatile terpenes, flavonoids and phenolic acids in Greek oregano (Origanum vulgare L. ssp. hirtum) at different development stages during cultivation in cool temperate climate. Eur J Hort Sci 74:193–203

    CAS  Google Scholar 

  • Kamaraj C, Rajakumar G, Rahuman AA, Velayutham K, Bagavan A, Zahir AA, Elango G (2012) Feeding deterrent activity of synthesized silver nanoparticles using Manilkara zapota leaf extract against the house fly, Musca domestica (Diptera: Muscidae). Parasitol Res 111:2439–2448

    Article  Google Scholar 

  • Kanda D, Kaur S, Koul O (2017) A comparative study of monoterpenoids and phenylpropanoids from essential oils against stored grain insects: acute toxins or feeding deterrents. J Pest Sci 90:531–545

    Article  Google Scholar 

  • Kim SI, Yoon JS, Jung JW, Hong KB, Ahn YJ, Kwon HW (2010) Toxicity and repellency of origanum essential oil and its components against Tribolium castaneum (Coleoptera: Tenebrionidae) adults. J Asia Pac Entomol 13:369–373

    Article  CAS  Google Scholar 

  • Kim JR, Haribalan P, Son BK, Ahn YJ (2012) Fumigant toxicity of plant essential oils against Camptomyia corticalis (Diptera: Cecidomyiidae). J Econ Entomol 105(4):1329–1334

    Article  Google Scholar 

  • Koul O, Singh R, Kaur B, Kanda D (2013) Comparative study on the behavioral response and acute toxicity of some essential oil compounds and their binary mixtures to larvae of Helicoverpa armigera, Spodoptera litura and Chilo partellus. Ind Crop Prod 49:428–436

    Article  CAS  Google Scholar 

  • Kumar P, Mishra S, Malik A, Satya S (2011) Repellent, larvicidal and pupicidal properties of essential oils and their formulations against the housefly, Musca domestica. Med Vet Entomol 25:302–310

    Article  CAS  Google Scholar 

  • Kumar P, Mishra S, Malik A, Satya S (2012a) Efficacy of Mentha × piperita and Mentha citrata essential oils against housefly, Musca domestica L. Ind Crop Prod 39:106–112

    Article  CAS  Google Scholar 

  • Kumar P, Mishra S, Malik A, Satya S (2012b) Insecticidal evaluation of essential oils of Citrus sinensis L. (Myrtales: Myrtaceae) against housefly, Musca domestica L. (Diptera: Muscidae). Parasitol Res 110:1929–1936

    Article  Google Scholar 

  • Kumar P, Mishra S, Malik A, Satya S (2013) Housefly (Musca domestica L.) control potential of Cymbopogon citratus Stapf. (Poales: Poaceae) essential oil and monoterpenes (citral and 1,8-cineole). Parasitol Res 112:69–76

    Article  Google Scholar 

  • Kumar P, Mishra S, Malik A, Satya S (2014) Biocontrol potential of essential oil monoterpenes against housefly, Musca domestica (Diptera: Muscidae). Ecotoxicol Environ Saf 100:1–6

    Article  CAS  Google Scholar 

  • Lawrence BM (1984) The botanical and chemical aspects of oregano. Perfum Flavor 9:41–51

    CAS  Google Scholar 

  • Lee HR, Kim GH, Choi WS, Park IK (2017) Repellent activity of Apiaceae plant essential oils and their constituents against adult German cockroaches. J Econ Entomol 110(2):552–557

    Article  CAS  Google Scholar 

  • Lima RK, Cardoso MG, Moraes JC, Carvalho SM, Rodrigues VG, Guimarães LGL (2011) Chemical composition and fumigant effect of essential oil of Lippia sidoides Cham and monoterpenes against Tenebrio molitor (L.) (Coleoptera: Tenebrionidae). Ciênc agrotec Lavras 35:664–671

    Article  CAS  Google Scholar 

  • López V, Cascella M, Benelli G, Maggi F, Gómez-Rincón C (2018) Green drugs in the fight against Anisakis simplex-larvicidal activity and acetylcholinesterase inhibition of Origanum compactum essential oil. Parasitol Res 117:861–867

    Article  Google Scholar 

  • Ma WB, Feng JT, Jiang ZL, Wu H, Ma ZQ, Zhang X (2014) Fumigant activity of eleven essential oil compounds and their selected binary mixtures against Culex pipiens pallens (Diptera: Culicidae). Parasitol Res 113:3631–3637

    Article  Google Scholar 

  • Mansour SA, El-Sharkawy AZ, Abdel-Hamid NA (2015) Toxicity of essential plant oils, in comparison with conventional insecticides, against the desert locust, Schistocerca gregaria (Forskål). Ind Crop Prod 63:92–99

    Article  CAS  Google Scholar 

  • Martino LD, Feo VD, Formisano C, Mignola E, Senatore F (2009) Chemical composition and antimicrobial activity of the essential oils from three chemotypes of Origanum vulgare L. ssp. hirtum (link) Ietswaart growing wild in Campania (southern Italy). Molecules 14:2735–2746

    Article  CAS  Google Scholar 

  • Meng H, Li AY, Junior LMC, Castro-Arellano I, Liu J (2016) Evaluation of DEET and eight essential oils for repellency against nymphs of the lone star tick, Amblyomma americanum (Acari: Ixodidae). Exp Appl Acarol 68:241–249

    Article  CAS  Google Scholar 

  • Morshedloo MR, Craker LE, Salami A, Nazeri V, Sang H, Maggi F (2017a) Effect of prolonged water stress on essential oil content, compositions and gene expression patterns of mono- and sesquiterpene synthesis in two oregano (Origanum vulgare L.) subspecies. Plant Physiol Bioch 111:119–128

    Article  CAS  Google Scholar 

  • Morshedloo MR, Mumivand H, Craker LE, Maggi F (2017b) Chemical composition and antioxidant activity of essential oils in Origanum vulgare subsp gracile at different phenological stages and plant parts J Food Process Preserv. e13516

  • Morshedloo MR, Salami SA, Nazeri V, Maggi F, Craker L (2018) Essential oil profile of oregano (Origanum vulgare L.) populations grown under similar soil and climate conditions. Ind Crop Prod 119:183–190

    Article  CAS  Google Scholar 

  • Muturi EJ, Ramirez JL, Doll KM, Bowman MJ (2017) Combined toxicity of three essential oils against Aedes aegypti (Diptera: Culicidae) larvae. J Med Entomol 54(6):1684–1691

    Article  CAS  Google Scholar 

  • Nuruzzaman M, Rahman MM, Liu YJ, Naidu R (2016) Nanoencapsulation, nano-guard for pesticides: a new window for safe application. J Agric Food Chem 64(7):1447–1483

    Article  CAS  Google Scholar 

  • Palacios SM, Bertoni A, Rossi Y, Santander R, Urzúa A (2009a) Insecticidal activity of essential oils from native medicinal plants of Central Argentina against the housefly, Musca domestica (L.). Parasitol Res 106:207–212

    Article  Google Scholar 

  • Palacios SM, Bertoni A, Rossi Y, Santander R, Urzúa A (2009b) Efficacy of essential oils from edible plants as insecticides against the house fly, Musca domestica L. Molecules 14:1938–1947

    Article  CAS  Google Scholar 

  • Pandey A, Chattopadhyay P, Banerjee S, Pakshirajan K, Singh L (2012) Antitermitic activity of plant essential oils and their major constituents against termite Odontotermes assamensis Holmgren (Isoptera: Termitidae) of north East India. Int Biodeterior Biodegradation 75:63–67

    Article  CAS  Google Scholar 

  • Park IK, Kim JN, Lee YS, Lee SG, Ahn YJ, Shin SC (2008) Toxicity of plant essential oils and their components against Lycoriella ingenua (Diptera: Sciaridae). J Econ Entomol 101:139–144

    Article  CAS  Google Scholar 

  • Park CG, Jang M, Yoon KA, Kim J (2016) Insecticidal and acetylcholinesterase inhibitory activities of Lamiaceae plant essential oils and their major components against Drosophila suzukii (Diptera: Drosophilidae). Ind Crop Prod 89:507–513

    Article  CAS  Google Scholar 

  • Pavela R (2008) Insecticidal properties of several essential oils on the house fly (Musca domestica L.). Phytother Res 22:274–278

    Article  CAS  Google Scholar 

  • Pavela R (2012) Sublethal effects of some essential oils on the cotton Leafworm Spodoptera littoralis (Boisduval). J Essent Oil Bear Pl 15:144–156

    Article  CAS  Google Scholar 

  • Pavela R (2014a) Insecticidal properties of Pimpinella anisum essential oils against the Culex quinquefasciatus and the non-target organism Daphnia magna. J Asia Pac Entomol 17:287–293

    Article  CAS  Google Scholar 

  • Pavela R (2014b) Acute, synergistic and antagonistic effects of some aromatic compounds on the Spodoptera littoralis Boisd. (Lep., Noctuidae) larvae. Ind Crop Prod 60:247–258

    Article  CAS  Google Scholar 

  • Pavela R (2015) Acute toxicity and synergistic and antagonistic effects of the aromatic compounds of some essential oils against Culex quinquefasciatus say larvae. Parasitol Res 114:3835–3853

    Article  Google Scholar 

  • Pavela R (2016) History, presence and perspective of using plant extracts as commercial botanical insecticides and farm products for protection against insects—a review. Plant Prot Sci 52:229–241

    Article  CAS  Google Scholar 

  • Pavela R, Benelli G (2016) Essential oils as eco-friendly biopesticides? Challenges and constraints. Trends Plant Sci 21:1000–1007

    Article  CAS  Google Scholar 

  • Pavela R, Govindarajan M (2017) The essential oil from Zanthoxylum monophyllum a potential mosquito larvicide with low toxicity to the non-target fish Gambusia affinis. J Pest Sci 90:369–378

    Article  Google Scholar 

  • Pavela R, Maggi F, Lupidi G, Mbuntcha H, Woguem V, Womeni HM, Barboni L, Tapondjou LA, Benelli G (2018) Clausena anisata and Dysphania ambrosioides essential oils: from ethno-medicine to modern uses as effective insecticides. Environ Sci Pollut Res 25:10493–10503

    Article  CAS  Google Scholar 

  • Pergola AL, Restuccia C, Napoli E, Bella S, Brighina S, Russo A, Suma P (2017) Commercial and wild Sicilian Origanum vulgare essential oils: chemical composition, antimicrobial activity and repellent effects. J Essent Oil Res 29(6):451–460

    Article  CAS  Google Scholar 

  • Rao GV, Mukhopadhyay T, Annamalai T, Radhakrishnan N, Sahoo MR (2011) Chemical constituents and biological studies of Origanum vulgare Linn. Pcog Res 3:143–145

    CAS  Google Scholar 

  • Rossi YE, Palacios SM (2015) Insecticidal toxicity of Eucalyptus cinerea essential oil and 1,8-cineole against Musca domestica and possible uses according to the metabolic response of flies. Ind Crop Prod 63:133–137

    Article  CAS  Google Scholar 

  • Sarikurkcu C, Zengin G, Oskay M, Uysal S, Ceylan R, Aktumsek A (2015) Composition, antioxidant, antimicrobial and enzyme inhibition activities of two Origanum vulgare subspecies (subsp. vulgare andsubsp. hirtum) essential oils. Ind Crop Prod 70:178–184

    Article  CAS  Google Scholar 

  • Sekine N, Shibutani S (2013) Chemical structures of p-menthane monoterpenes with special reference to their effect on seed germination and termite mortality. J Wood Sci 59:229–237

    Article  CAS  Google Scholar 

  • Seo SM, Park IK (2012) Larvicidal activity of medicinal plant extracts and lignin identified in Phryma leptostachya var. asiatica roots against housefly (Musca domestica L.). Parasitol Res 110:1849–1853

    Article  Google Scholar 

  • Seo SM, Park HM, Park IK (2012) Larvicidal activity of Ajowan (Trachyspermum ammi) and Peru balsam (Myroxylon pereira) oils and blends of their constituents against mosquito, Aedes aegypti, acute toxicity on water flea, Daphnia magna, and aqueous residue. J Agric Food Chem 60:5909–5914

    Article  CAS  Google Scholar 

  • Seo SM, Jung CS, Kang J, Lee HR, Kim S.W, Hyun J, Park IK (2015) Larvicidal and acetylcholinesterase inhibitory activities of Apiaceae plant essential oils and their constituents against Aedes albopictus and formulation development. J Agric Food Chem 63:9977–9986

  • Sharififard M, Safdari F, Siahpoush A, Kassiri H (2016) Evaluation of some plant essential oils against the Brown-banded cockroach, Supella longipalpa (Blattaria: Ectobiidae): a mechanical vector of human pathogens. J Arthropod Borne Dis 10:528–537

    Google Scholar 

  • Szczepanik M, Walczak M, Zawitowska B, Michalska-Sionkowska M, Szumny A, Wawrzeńczyk C, Brzezinska MS (2018) Chemical composition, antimicromicrobial activity and insecticidal activity against the lesser mealworm Alphitobius diaperinus (panzer) (Coleoptera: Tenebrionidae) of Origanum vulgare L. ssp. hirtum (link) and Artemisia dracunculus L. essential oils. J Sci Food Agric 98:767–774

    Article  CAS  Google Scholar 

  • Tabanca N, Bernier UR, AliA WM, Demirci B, Blythe EK, Khan SI, Baser KHC, Khan IA (2013) Bioassay-guided investigation of two Monarda essential oils as repellents of yellow fever mosquito Aedes aegypti. J Agric Food Chem 61:8573–8580

    Article  CAS  Google Scholar 

  • Tabari MA, Youssefi MR, Maggi F, Benelli G (2017) Toxic and repellent activity of selected monoterpenoids (thymol, carvacrol and linalool) against the castor bean tick, Ixodes ricinus (Acari: Ixodidae). Vet Parasitol 245:86–91

    Article  CAS  Google Scholar 

  • Tak JH, Isman MB (2017) Acaricidal and repellent activity of plant essential oil-derived terpenes and the effect of binary mixtures against Tetranychus urticae Koch (Acari: Tetranychidae). Ind Crop Prod 108:786–792

    Article  CAS  Google Scholar 

  • Wang HW, Liu YQ, Wei SL, Yan ZJ (2012) Comparative seasonal variation and chemical composition of essential oils from the leaves and stems of Schefflera heptaphylla using microwave-assisted and conventional hydrodistillation. Ind Crop Prod 36:229–237

    Article  CAS  Google Scholar 

  • Wijesundara NM, Rupasinghe HPV (2018) Essential oils from Origanum vulgare and Salvia officinalis exhibit antibacterial and anti-biofilm activities against Streptococcus pyogenes. Microb Pathog 117:118–127

    Article  CAS  Google Scholar 

  • Xie YJ, Huang QY, Yang FL, Lei CL (2012) Chemical variation in essential oil of Cryptomeria fortunei from various areas of China. Ind Crop Prod 36:308–312

    Article  CAS  Google Scholar 

  • Xie YJ, Wang K, Huang QY, Lei CL (2014) Evaluation toxicity of monoterpenes to subterranean termite, Reticulitermes chinensis Snyder. Ind Crop Prod 53:163–166

    Article  CAS  Google Scholar 

  • Xie YJ, Wang ZJ, Huang QQ, Zhang DY (2017) Antifungal activity of several essential oils and major components against wood-rot fungi. Ind Crop Prod 108:278–285

    Article  CAS  Google Scholar 

  • Yeom HJ, Kang JS, Kim GH, Park IK (2012) Insecticidal and acetylcholine esterase inhibition activity of Apiaceae plant essential oils and their constituents against adults of German cockroach (Blattella germanica). J Agric Food Chem 60:7194–7203

    Article  CAS  Google Scholar 

  • Yoon C, Moon SR, Jeong JW, Shin YH, Cho SR, Ahn KS, Yang JO, Kim GH (2011) Repellency of lavender oil and linalool against spot clothing wax cicada, Lycorma delicatula (Hemiptera: Fulgoridae) and their electrophysiological responses. J Asia Pac Entomol 14:411–416

    Article  CAS  Google Scholar 

  • Zahran HEDM, Abou-Talebb HK, Abdelgaleil SAM (2017) Adulticidal, larvicidal and biochemical properties of essential oils against Culex pipiens L. J Asia Pac Entomol 20:133–139

    Article  Google Scholar 

  • Zhang ZL, Xie YJ, Wang Y, Lin ZF, Wang LH, Li GY (2017) Toxicities of monoterpenes against housefly, Musca domestica L. (Diptera: Muscidae). Environ Sci Pollut Res 24:24708–24713

    Article  CAS  Google Scholar 

  • Zhao X, Cui HX, Wang Y, Sun CJ, Cui B, Zeng ZH (2018) Development strategies and prospects of nano-based smart pesticide formulation. J Agric Food Chem 66(26):6504–6512

    Article  CAS  Google Scholar 

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Acknowledgments

This work was supported by Zhejiang Provincial Natural Science Foundation of China under grant number LQ14C040003 and Zhejiang A&F University research fund for financial supports (nos. 2012FR087, 2014FR009).

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  1. School of Agricultural and Food Science, Zhejiang A&F University, Hangzhou, 311300, People’s Republic of China

    Yongjian Xie, Qianqian Huang, Yuqing Rao, Liang Hong & Dayu Zhang

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Xie, Y., Huang, Q., Rao, Y. et al. Efficacy of Origanum vulgare essential oil and carvacrol against the housefly, Musca domestica L. (Diptera: Muscidae). Environ Sci Pollut Res 26, 23824–23831 (2019). https://doi.org/10.1007/s11356-019-05671-4

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