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An enhancement of antimicrobial efficacy of biogenic and ceftriaxone-conjugated silver nanoparticles: green approach

  • Plant-borne compounds and nanoparticles: challenges for medicine, parasitology and entomology
  • Published:
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Abstract

Of the various methods explored for the synthesis of nanoparticles, biogenesis of silver nanoparticles (AgNPs) received great attention due to their versatile properties. In this report, Daucus carota extract was used for the synthesis of AgNPs and ceftriaxone was conjugated with AgNPs to enhance their antimicrobial efficacy. The conjugated and unconjugated AgNPs were characterized by adopting UV-Vis spectroscopy, FTIR, AFM, DLS, and TEM, which revealed the SPR peak at 420 nm and spherical shaped nanoparticles of 20 nm size, respectively. The antimicrobial efficacies of the unconjugated AgNPs and ceftriaxone-conjugated AgNPs were tested against ceftriaxone-resistant human pathogens, Bacillus cereus, Staphylococcus aureus, Klebsiella pneumoniae, and Pseudomonas aeruginosa. The ceftriaxone-conjugated AgNPs showed high inhibitory action (23 mm) than the unconjugated AgNPs (18 mm) at the concentration of 50 μg/mL. Both the unconjugated and ceftriaxone-conjugated AgNPs were found to be non-toxic on EAC cells at 50 μg/mL. The dose-dependent cytotoxic activities were observed on increasing the concentration of the AgNPs. The ceftriaxone-conjugated AgNPs showed high activity than the unconjugated AgNPs. The enhanced activity could be useful to treat ceftriaxone-resistant human pathogens.

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References

  • Baüer AW, Kirby WM, Sherris JC, Turck M (1966) Antibiotic susceptibility testing by a standardized single disk method. J Clin Pathol 45:493–496

    Article  Google Scholar 

  • Christy AJ, Umadevi M (2012) Synthesis and characterization of monodispersed silver nanoparticles. Adv Nat Sci Nanosci Nanotechnol 3:035013

    Article  Google Scholar 

  • Durán N, Marcato PD, Alves OL et al (2005) Mechanistic aspects of biosynthesis of silver nanoparticles by several Fusarium oxysporum strains. J Nanobiotechnol 3:8

    Article  Google Scholar 

  • Dutta PP, Bordoloi M, Gogoi K et al (2017) Antimalarial silver and gold nanoparticles: green synthesis, characterization and in vitro study. Biomed Pharmacother 91:567–580

    Article  CAS  Google Scholar 

  • Gomaa EZ (2017) Antimicrobial, antioxidant and antitumor activities of silver nanoparticles synthesized by Allium cepa extract: a green approach. J Genet Eng Biotechnol. doi:10.1016/j.jgeb.2016.12.002

  • Gopinath V, MubarakAli D, Priyadarshini S, Meera Priyadharsshini S, Thajuddin N, Velusamy P (2012) Biosynthesis of silver nanoparticles from Tribulus terrestris and its antimicrobial activity: a novel biological approach. Colloids Surf B: Biointerfaces 96:69–74

    Article  CAS  Google Scholar 

  • Gopinath V, Priyadarshini S, Loke MF et al (2015) Biogenic synthesis, characterization of antibacterial silver nanoparticles and its cell cytotoxicity. Arab J Chem. doi:10.1016/j.arabjc.2015.11.011

  • Harshiny M, Matheswaran M, Arthanareeswaran G et al (2015) Enhancement of antibacterial properties of silver nanoparticles–ceftriaxone conjugate through Mukia maderaspatana leaf extract mediated synthesis. Ecotoxicol Environ Saf 121:135–141

    Article  CAS  Google Scholar 

  • Iravani S, Korbekandi H, Mirmohammadi SV, Zolfaghari B (2014) Synthesis of silver nanoparticles: chemical, physical and biological methods. Res Pharm Sci 9:385–406

    CAS  Google Scholar 

  • Jacob SJP, Finub JS, Narayanan A (2012) Synthesis of silver nanoparticles using Piper longum leaf extracts and its cytotoxic activity against Hep-2 cell line. Colloids Surf B Biointerfaces 91:212–214

    Article  Google Scholar 

  • Jeyaraj M, Sathishkumar G, Sivanandhan G, MubarakAli D et al (2013) Biogenic silver nanoparticles for cancer treatment: an experimental report. Colloids Surf B Biointerfaces 106:86–92

    Article  CAS  Google Scholar 

  • Kanmani P, Lim ST (2013) Synthesis and structural characterization of silver nanoparticles using bacterial exopolysaccharide and its antimicrobial activity against food and multidrug resistant pathogens. Process Biochem 48:1099–1106

    Article  CAS  Google Scholar 

  • Liu L, Yang J, Xie J et al (2013) The potent antimicrobial properties of cell penetrating peptide-conjugated silver nanoparticles with excellent selectivity for gram-positive bacteria over erythrocytes. Nano 5:3834–3840

    CAS  Google Scholar 

  • Logeswari P, Silambarasan S, Abraham J (2013) Ecofriendly synthesis of silver nanoparticles from commercially available plant powders and their antibacterial properties. Scientia Iranica 20:1049–1054

    Google Scholar 

  • Lok C-N, Ho C-M, Chen R et al (2006) Proteomic analysis of the mode of antibacterial action of silver nanoparticles. J Proteome Res 5:916–924

    Article  CAS  Google Scholar 

  • Maráková N, Humpolíček P, Kašpárková V et al (2017) Antimicrobial activity and cytotoxicity of cotton fabric coated with conducting polymers, polyaniline or polypyrrole, and with deposited silver nanoparticles. Appl Surf Sci 396:169–176

    Article  Google Scholar 

  • Mohanty S, Mishra S, Jena P et al (2012) An investigation on the antibacterial, cytotoxic, and antibiofilm efficacy of starch-stabilized silver nanoparticles. Nanomed Nanotech Biol Med 8:916–924

    Article  CAS  Google Scholar 

  • MubarakAli D, Thajuddin N, Jeganathan K, Gunasekaran M (2011) Plant extract mediated synthesis of silver and gold nanoparticles and its antibacterial activity against clinically isolated pathogens. Colloids Surf B Biointerfaces 85:360–365

    Article  CAS  Google Scholar 

  • MubarakAli D, Sang-Yul L, Seong-Cheol K, Jung-Wan K (2015) One-step synthesis of cellulose/silver nanobiocomposites using a solution plasma process and characterization of their broad spectrum antimicrobial efficacy. RSC Adv 5:35052–35060

    Article  Google Scholar 

  • MubarakAli D, Seong-Cheol K, Sang-Yul L, Jung-Wan K (2016) The facile synthesis of chitosan-based silver nano-biocomposites via a solution plasma process and their potential antimicrobial efficacy. Arch Biochem Biophys 605:49–58

    Article  Google Scholar 

  • Muthu K, Priya S (2017) Green synthesis, characterization and catalytic activity of silver nanoparticles using Cassia auriculata flower extract separated fraction. Spectrochim Acta Mol Biomol Spectrosc 179:66–72

    Article  CAS  Google Scholar 

  • Ostaszewska T, Chojnacki M, Kamaszewski M, Sawosz-Chwalibóg E (2016) Histopathological effects of silver and copper nanoparticles on the epidermis, gills, and liver of Siberian sturgeon. Environ Sci Pollut Res Int 23:1621–1633

    Article  CAS  Google Scholar 

  • Pugazhendhi S, Kirubha E, Palanisamy PK, Gopalakrishnan R (2015) Synthesis and characterization of silver nanoparticles from Alpinia calcarata by Green approach and its applications in bactericidal and nonlinear optics. Appl Surf Sci 357:1801–1808

    Article  CAS  Google Scholar 

  • Pugazhendhi S, Sathya P, Palanisamy PK, Gopalakrishnan R (2016) Synthesis of silver nanoparticles through green approach using Dioscorea alata and their characterization on antibacterial activities and optical limiting behavior. J Photochem Photobiol B 159:155–160

    Article  CAS  Google Scholar 

  • Rajakumar G, Abdul Rahuman A (2011) Larvicidal activity of synthesized silver nanoparticles using Eclipta prostrata leaf extract against filariasis and malaria vectors. Acta Trop 118:196–203

    Article  CAS  Google Scholar 

  • Ramkumar VS, Pugazhendhi A, Gopalakrishnan K et al (2017) Biofabrication and characterization of silver nanoparticles using aqueous extract of seaweed Enteromorpha compressa and its biomedical properties. Biotechnol Rep 14:1–7

    Article  Google Scholar 

  • Sahana R, Daniel SCGK, Sankar SG et al (2014) Formulation of bactericidal cold cream against clinical pathogens using Cassia auriculata flower extract-synthesized Ag nanoparticles. Green Chem Lett Rev 7:64–72

    Article  Google Scholar 

  • Salem JK, El-Nahhal IM, Najri BA et al (2016) Effect of anionic surfactants on the surface plasmon resonance band of silver nanoparticles: determination of critical micelle concentration. J Mol Liq 223:771–774

    Article  CAS  Google Scholar 

  • Sangwoo N, MubarakAli D, Jungwan K (2016) Characterization of alginate/silver nanobiocomposites synthesized by solution plasma process and their antimicrobial properties. J Nanomater 4712813:9

    Google Scholar 

  • Saratale GD, Saratale RG, Benelli G et al (2017) Anti-diabetic potential of silver nanoparticles synthesized with Argyreia nervosa leaf extract high synergistic antibacterial activity with standard antibiotics against foodborne bacteria. J Clust Sci 28:1709–1727

    Article  CAS  Google Scholar 

  • Sarkar S, Jana AD, Samanta SK, Mostafa G (2007) Facile synthesis of silver nano particles with highly efficient anti-microbial property. Polyhedron 26:4419–4426

    Article  CAS  Google Scholar 

  • Shankar PD, Shobana S, Karuppusamy I et al (2016) A review on the biosynthesis of metallic nanoparticles (gold and silver) using bio-components of microalgae: formation mechanism and applications. Enzym Microb Technol 95:28–44

    Article  CAS  Google Scholar 

  • Shrivastava S, Bera T, Roy A et al (2007) Characterization of enhanced antibacterial effects of novel silver nanoparticles. Nanotechnology 18:225103

    Article  Google Scholar 

  • Singh D, Rathod V, Ninganagouda S et al (2014) Optimization and characterization of silver nanoparticle by endophytic fungi Penicillium sp. isolated from Curcuma longa (turmeric) and application studies against MDR E. coli and S. aureus. Bioinorg Chem Appl 2014:e408021

    Google Scholar 

  • Sondi I, Salopek-Sondi B (2004) Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for gram-negative bacteria. J Colloid Interface Sci 275:177–182

    Article  CAS  Google Scholar 

  • Sperling RA, Parak WJ (2010) Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles. Philos Trans R Soc Lond A 368:1333–1383

    Article  CAS  Google Scholar 

  • Su Y, Zhao L, Meng F et al (2017) Silver nanoparticles decorated lipase-sensitive polyurethane micelles for on-demand release of silver nanoparticles. Colloids Surf B: Biointerfaces 152:238–244

    Article  CAS  Google Scholar 

  • Suman TY, Radhika Rajasree SR, Kanchana A, Elizabeth SB (2013) Biosynthesis, characterization and cytotoxic effect of plant mediated silver nanoparticles using Morinda citrifolia root extract. Colloids Surf B: Biointerfaces 106:74–78

    Article  CAS  Google Scholar 

  • Thiel J, Pakstis L, Buzby S et al (2007) Antibacterial properties of silver-doped titania. Small 3:799–803

    Article  CAS  Google Scholar 

  • Venugopal K, Rather HA, Rajagopal K et al (2017) Synthesis of silver nanoparticles (Ag NPs) for anticancer activities (MCF 7 breast and A549 lung cell lines) of the crude extract of Syzygium aromaticum. J Photochem Photobiol B Biol 167:282–289

    Article  CAS  Google Scholar 

  • Vijayan SR, Santhiyagu P, Ramasamy R et al (2016) Seaweeds: a resource for marine bionanotechnology. Enzym Microb Technol 95:45–57

    Article  CAS  Google Scholar 

  • Yohannan Panicker C, Tresa Varghese H, Philip D (2006) FT-IR, FT-Raman and SERS spectra of vitamin C. Spectrochim Acta Mol Biomol Spectrosc 65:802–804

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors would like to acknowledge DST-FIST for providing the instrumentation facilities for characterization studies. Department of Biotechnology (DBT, Govt. Of India) is gratefully acknowledged for NRMC-F.

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Authors and Affiliations

  1. CoRx Life sciences and Pharmaceutical (CLAP) Private Limited, Tiruchirappalli, Tamil Nadu, 620 020, India

    Rajasree Shanmuganathan

  2. National Repository for Microalgae and Cyanobacteria - Freshwater (DBT), Department of Microbiology, Bharathidasan University, Tiruchirappalli, Tamil Nadu, 620 024, India

    Davoodbasha MubarakAli & Nooruddin Thajuddin

  3. Anna University, ACT Campus, Chennai, Tamil Nadu, 25, India

    Desika Prabakar

  4. Department of Biotechnology, Periyar Maniammai University, Thanjavur, Tamil Nadu, 613 403, India

    Harshiny Muthukumar

  5. Department of Environmental Science and Engineering, Guru Jambheshwar University of Science and Technology, Hisar, Haryana, 125001, India

    Smita S. Kumar

  6. Green Processing, Bioremediation and Alternative Energies Research Group (GPBAE), Faculty of Environment and Labour Safety, Ton Duc Thang University, Ho Chi Minh City, Vietnam

    Arivalagan Pugazhendhi

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  1. Rajasree Shanmuganathan
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  2. Davoodbasha MubarakAli
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  3. Desika Prabakar
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  4. Harshiny Muthukumar
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  5. Nooruddin Thajuddin
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  6. Smita S. Kumar
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  7. Arivalagan Pugazhendhi
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Correspondence to Arivalagan Pugazhendhi.

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Shanmuganathan, R., MubarakAli, D., Prabakar, D. et al. An enhancement of antimicrobial efficacy of biogenic and ceftriaxone-conjugated silver nanoparticles: green approach. Environ Sci Pollut Res 25, 10362–10370 (2018). https://doi.org/10.1007/s11356-017-9367-9

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  • DOI: https://doi.org/10.1007/s11356-017-9367-9

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