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Cadmium impact, accumulation and detection in poplar callus cells

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Abstract

Effect of cadmium cations and their interaction with silicon cations was determined in poplar calli and expressed as changes in callus growth, cell viability and cadmium cation accumulation. Cell viability throughout culture versus cadmium cation accumulation in cells is discussed. At the same time, the study sought appropriate methods for cadmium cation detection in callus cells and also in experiments with low plant material (e.g. protoplasts). Cadmium cations were determined by atomic absorption spectroscopy and using fluorescence microscopy with a specific cadmium cation fluorescent dye. The detection of cadmium cations in callus cells by the latter method appears suitable because the callus cells are surrounded by primary cell walls without auto-fluorescence and these values fit well with atomic absorption spectroscopy quantification. However, the visualisation method has some limits discussed below.

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Abbreviations

AAS:

Atomic absorption spectroscopy

Cd:

Cadmium cations

LOD:

Detection limit

LOQ:

Quantitation limit

Si:

Silicon cations

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Acknowledgements

This work was financially supported by the Scientific Grant Agency of the Ministry of Education of the Slovak Republic and the Academy of Sciences VEGA no. 2/0083/14 and the Slovak Research and Development Agency, grant no. APVV-0140-10. The authors thank Dr. Marek Bujdoš for providing the expertise on Cd and Si determination by AAS analysis in plant material.

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Authors and Affiliations

  1. Department of Glycobiotechnology, Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38, Bratislava, Slovakia

    Karin Kollárová, Zuzana Vatehová, Danica Kučerová & Desana Lišková

Authors
  1. Karin Kollárová
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  2. Zuzana Vatehová
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  3. Danica Kučerová
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  4. Desana Lišková
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Correspondence to Karin Kollárová.

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Responsible editor: Elena Maestri

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Kollárová, K., Vatehová, Z., Kučerová, D. et al. Cadmium impact, accumulation and detection in poplar callus cells. Environ Sci Pollut Res 24, 15340–15346 (2017). https://doi.org/10.1007/s11356-017-9158-3

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