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Concentration-dependent alterations in gene expression induced by cadmium in Solanum lycopersicum

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Abstract

Cadmium (Cd) toxicity in agricultural soil has received significant attention because of its higher transformation in the food chain and toxicity to humans. The aim of the present study was to develop sensitive and specific biomarkers for Cd stress. Therefore, transcriptional analyses were performed to investigate concentration-response characteristics of Cd responsive genes identified from a Solanum lycopersicum microarray. The results showed that the lowest observable adverse effect concentrations (LOAECs) of Cd to S. lycopersicum were 1 mg/kg for seed germination, 8 mg/kg for root dry weight, 8 mg/kg for root elongation, and 8 mg/kg for root morphology. Furthermore, the genes were differentially expressed even at the lowest Cd concentrations (0.5 mg/kg), indicating that the detection of Cd in soil at the molecular level is a highly sensitive method. Cd in soil was positively correlated with the expression of the F-box protein PP2-B15 (r = 0.809, p < 0.01) and zinc transporter 4 (r = 0.643, p < 0.01), indicating that these two genes could be selected as indicators of soil Cd contamination.

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Acknowledgments

National Basic Research Program of China (2013CB430400); the National Natural Science Foundation of China (21377013, 21607043); the Fundamental Research Funds for the Central Universities (2016ZZD06); and the Open Project of Key Laboratory of Environmental Biotechnology, CAS (Grant No. kf2016009), provided the financial support.

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Authors and Affiliations

  1. School of Environment And Chemical Engineering, North China Electric Power University, Beijing, 102206, China

    Jing Hou & Xiangke Wang

  2. State Key Laboratory of Water Environment Simulation, School of Environment, Beijing Normal University, Beijing, 100875, China

    Xinhui Liu, Baoshan Cui & Junhong Bai

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  1. Jing Hou
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  2. Xinhui Liu
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  3. Baoshan Cui
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  4. Junhong Bai
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  5. Xiangke Wang
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Correspondence to Xinhui Liu or Xiangke Wang.

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Responsible editor: Yi-ping Chen

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Hou, J., Liu, X., Cui, B. et al. Concentration-dependent alterations in gene expression induced by cadmium in Solanum lycopersicum . Environ Sci Pollut Res 24, 10528–10536 (2017). https://doi.org/10.1007/s11356-017-8748-4

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