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Preparation, characterization, and bio-efficacy evaluation of controlled release carbendazim-loaded polymeric nanoparticles

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Abstract

Synthesis and controlled release study of polymeric nanoformulation of carbendazim (2-benzimidazole carbamic acid methyl ester) using chitosan and pectin is reported in this article. The formulation was subjected to morphological, physiological, in vitro fungicide release and bio-efficacy evaluation studies. The average size of nanoparticles was found to be in the range of 70–90 nm as confirmed by transmission electron microscopy. The in vitro fungicide release of nanoformulated carbendazim was compared with pure carbendazim at different pH values. The results confirmed sustained release of nanoformulated carbendazim. The bio-efficacy evaluation of the carbendazim nanoformulation was carried out against Fusarium oxysporum and Aspergillus parasiticus. The nanoformulation showed 100 % inhibition of test fungi at both concentrations (0.5 and 1.0 ppm) while pure carbendazim showed 80 ± 0 % and 97.2 ± 1.1 % inhibition at 0.5 and 1.0 ppm concentration respectively against Fusarium oxysporum and 86.0 ± 0.6 % inhibition and 100.0 % inhibition at 0.5 and 1.0 ppm concentration respectively against Aspergillus parasiticus. The commercial formulation (WP 50) showed 42 % and 58.0 ± 0.1 % inhibition at 0.5 and 1 ppm concentration respectively against Aspergillus parasiticus and 50.5 ± 0.7 % and 70.0 ± 0 % inhibition at 0.5 and 1.0 ppm concentrations respectively against Fusarium oxysporum. Phytotoxicity evaluation of nanoformulated fungicide confirmed that the nanoformulated carbendazim is safer for germination and root growth of the seeds of Cucumis sativa, Zea mays, and Lycopersicum esculantum.

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References

  • Aspden TJ, Mason JD, Jones NS (1997) Chitosan as a nasal delivery system: the effect of chitosan solutions on in vitro and in vivo mucociliary transport rates in human turbinates and volunteers. J Pharm Sci 86:509–513. doi:10.1021/js960182o

    Article  CAS  Google Scholar 

  • Bigucci F, Luppi B, Cerchiara T (2008) Chitosan/pectin polyelectrolyte complexes: selection of suitable preparative conditions for colon-specific delivery of vancomycin. Eur J Pharm Sci 35:435–441. doi:10.1016/j.ejps

    Article  CAS  Google Scholar 

  • Davidse LC (1986) Benzimidazole fungicide: mechanism of action and biological impact: a review. J Phytopathol 24:43–65. doi:10.1146/ annrev .py.24.090186.000355

    Article  CAS  Google Scholar 

  • Devi N, Maji T (2010) Genipin crosslinked chitosan –ҡ- carrageenan polyelectrolyte nanocapsules for the controlled delievery of isoniazid. International Journal of polymeric materials and polymeric biomaterials 59(10):828–841. doi:10.1080/00914037.2010.484792

    Article  CAS  Google Scholar 

  • Dhanya AT, Haridas K R, Divia N, Sudheesh (2012), Development of Zein-Pectin Nanoparticle as Drug carrier, Int J Drug Deliv. 147–152. http://www.arjournals.org/index.php/ijdd/index.

  • GeetaNegi, Pankaj, Anjana Srivastava, Anita Sharma (2014), In situ biodegradation of endosulfan, imidacloprid and carbendazim using indigenous bacterial cultures of agriculture fields of Uttarakhand, India, Int J Biological, Biomolecular, Agricultural, Food and Biotechnological Engineering Vol:8, No:9 international science index/ org/publication/9999239

  • Jana S, Gandhi A, Sen KK, Basu SK (2011) Natural polymers and their application in drug delivery and biomedical field. J Pharma Sci Tech 1(1):16–27 http://www.jpbms.info

    Google Scholar 

  • Jaya S, Durance TD, Wang R (2010) Physical characterization of drug loaded microcapsules and controlled in vitro release study. the J Open Biomaterials 2:9–17. doi:10.2174/1876502501002010009

    Article  CAS  Google Scholar 

  • Jia L, Wong H, Wang Y, Garza M, Weitman SD (2003) Carbendazim: disposition, cellular permeability, metabolite identification, and pharmacokinetic comparison with its nanoparticles. J Pharmasci 92. doi:10.1002/jps.10272

  • Kaur H, Ahuja M, Kumar S, Dilbaghi N (2012) Carboxymethyl tamarind kernel polysaccharide nanoparticles for ophthalmic drug delivery. Int J Bio Macromol 50:833–839. doi:10.1016/j.ijbiomac

    Article  CAS  Google Scholar 

  • Kibria G, YousufHaroon AK, Nugegoda D, Rose G (2010) Climate change and chemicals, environmental and biological aspects. New India Publishing Agency, Pitam Pura, New Delhi, p. 460

    Google Scholar 

  • Koli P, Singh BB, Shakil NA, Kumar J, Deebakamil (2015) Devolopment of controlled release nanoformulations of carbendazim employing amphiphilic polymers and their bioefficacy evaluation against Rhizoctonia solani. J Environ Sci Health 50(9):674–681. doi:10.1080/03601234.2015.1038961

    CAS  Google Scholar 

  • Komarek M, Cadkova E, Chrastny VB, Bollinger F (2010) Contamination of vineyard soils with fungicides: a review of environmental and toxicological aspects. Int J Environ 36:138–151. doi:10.1016/j.envint.2009.10.005

    Article  CAS  Google Scholar 

  • Kookana RS, Baskaran S, Naidu R (1998) Pesticide fate and behavior in Australian soils in relation to contamination and management of soil and water: a review, Aust. J Soil Res 36(5):715–764

    Article  CAS  Google Scholar 

  • Kuma S, Bhanjana G, Sharma A, Sidhu MC, Dilbaghi N (2014) Synthesis, characterization and on field evaluation of pesticide loaded sodium alginate nanoparticles. Carbohydr Polym 101:1061–1067. doi:10.1016/j.carbpol.2013.10.025

    Article  Google Scholar 

  • Kumar S, Chauhan N, Gopal M, Dilbaghi N (2015) Development and evaluation of alginate chitosan nanocapsules for controled release of acetamiprid. International J Bio Macromol 81. doi:10.1016/j.ijbiomac.2015.08.062

  • Leenheers LH, Englel R, Spruit WE, Meuling WJ, Jongen MJ (1993) Determination of methyl 5 hydroxy-2-benzimidazole carbamete in urine by high performance chromatography with electrochemical detection. J Chromatography 613(1):89–94

    Article  CAS  Google Scholar 

  • Liu L, Fishman ML, Hicks KB (2007) Pectin in controlled drug delivery: a rev. Cellulose 14:15–24. doi:10.1007/s10570-006-9095-7

    Article  Google Scholar 

  • Luo D-Q, Shao H-J, Zhu H-J, Liu J-K (2005) Activity in vitro and in vivo against plant pathogenic fungi of grifolin isolated from the basidiomycetes albatrellus dispansus. Zeitschriftfür Naturforschung C 60(1–2):50–56. doi:10.1515/znc-2005-1-210

    CAS  Google Scholar 

  • Mao HQ, Troung-le VL, Janes KA, Roy K, Wang Y, August JT, Leong KW (2001) Chitosan-DNA nanoparticles as gene carriers: synthesis, characterization and transfection efficiency. J Control Release 70:399–421. doi:10.1016/S0168-3659(00)00361-8

    Article  CAS  Google Scholar 

  • Mishra RK, Anis A, Mondal S, Dutt M, Banthia AK (2009) Preparation and characterization of amidated pectin based polymer electrolyte membranes, Chinese Journal of Polymeric. Sciences 27(5):639–646

    CAS  Google Scholar 

  • Mishra RK, Majeed ABA, Banthia AK (2011) Development and characterization of pectin/gelatin hydrogel membranes for wound dressing. Int J Plastics Technol 15(1):82–95. doi:10.1007/s12588-011-9016-y

    Article  CAS  Google Scholar 

  • Nagavarma B V N, Hemant K. S. Yadav, Ayaz A, Vasudha L. S., Shivakumar H. G. (2012), Different techniques for preparation of polymeric nanoparticles – A rev, Asian J Pharm and Clin Res,(5) 0974, 244.

  • Ni N, Sanghvi T, Samuel H, Yalkowsky (2002) Solubilization and preformulation of carbendazim. Int J Pharma 244(1–2):99–104. doi:10.1016/S0378-5173(02)00318-6

    Article  CAS  Google Scholar 

  • Priya C, Rajkumari, Ankita K (2013) Polymer: a boon to controlled drug delivery system. Int Res J Pharm 2230-8407. doi:10.7897/2230-8407.04405

  • Rani R, Dilbaghi N, Dhingra D, Kumar S (2015) Optimization and evaluation of bioactive drug - loaded polymeric nanoparticles for drug delivery. Int J Bio Macromol 78:173–179. doi:10.1016/ j.ijbiomac. 2015.3.070

    Article  CAS  Google Scholar 

  • Silva SML, Braga CRC, Fook MVL, Raposo CMO, Carvalhoand LH, Canedo EL (2012) Application of infrared spectroscopy to analysis of chitosan/clay nanocomposites. Journal of Materials Sci. Engineering and Techno 43-62. doi:10.5772/35522

  • Souza MP, Vaz AFM, Correia MTS, Cerqueira MA, Vicente AA, Carneiro-da-Cunha MG (2014) Quercetin loaded lecithin / chitosan nanoparticles for functional food applications. Food Bioprocess Technol l7:1149–1159. doi:10.1007/s11947-013-1160-2

    Article  Google Scholar 

  • Suresh Kumar RS, Shiny PJ, Anjali CH, Jerobin J, Goshen KM, Magdassi S, Mukherjee A, Chandrasekaran N (2012) Distinctive effects of nano-sized permethrin in the environment. Envir Sci Pollution Res 20:2593–2602. doi:10.1007/s11356-012-1161-0

    Article  Google Scholar 

  • Wightwick A, Salzman SA, Reichmann SM, Allinson G, Menzies NW (2013) Effects of copper fungicide residues on the microbial function of vineyard soils. Environ Sci and Pollution Res 20(3):1574–1585. doi:10.1007/s11356-012-1114

    Article  CAS  Google Scholar 

  • Wu J, Wei H, Sui X, Lin J, Wang T, Fen G, Xue J (2010) Bull Environ. Contam. Toxicol 84(4):469–472. doi:10.1007/s00128-010-9970-7

    CAS  Google Scholar 

  • Yearla SR, Padmasree K (2016) Exploitation of subabul stem lignin as a matrix in controlled release agrochemical nanoformulations: a case study with herbicide diuron. Enviorn sci and Pollution Res 6983-8. doi:10.1007/s11356-016-6983-8

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Acknowledgments

The authors thank DST Nano mission for the financial support in the form of HRD Grant and GJUS&T Hisar for innovative project grant.

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Author notes

  1. Dinesh Kumar

    Present address: Vice Chancellor, YMCA University of Science &Technology, Faridabad, India

Authors and Affiliations

  1. Department of Electronic Science, Kurukshetra University, Kurukshetra, 136119, India

    Sandhya & Dinesh Kumar

  2. Department of Bio & NanoTechnology, Guru Jambheshwar University of Science and Technology, Hisar, 250001, India

    Sandeep Kumar & Neeraj Dilbaghi

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  1. Sandhya
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  2. Sandeep Kumar
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  4. Neeraj Dilbaghi
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Correspondence to Neeraj Dilbaghi.

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Responsible editor: Thomas D. Bucheli

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Sandhya, Kumar, S., Kumar, D. et al. Preparation, characterization, and bio-efficacy evaluation of controlled release carbendazim-loaded polymeric nanoparticles. Environ Sci Pollut Res 24, 926–937 (2017). https://doi.org/10.1007/s11356-016-7774-y

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