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Inhibition and recovery of biomarkers of earthworm Eisenia fetida after exposure to thiacloprid

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Abstract

Thiacloprid, a neonicotinoid insecticide, has been used widely in agriculture worldwide. In recent years, the adverse effects of neonicotinoid insecticides on non-target organisms have attracted more and more attention. In the present study, effects of thiacloprid on molecular biomarkers (GST, CarE, CAT, SOD, POD, and DNA damage) of earthworm Eisenia fetida were investigated using the artificial OECD soil for the first time. Earthworms were exposed to thiacloprid (1 and 3 mg/kg) for 7, 14, and 28 days and then transferred to the clean OECD soil for 35, 42, and 56 days. Results showed that activities of GST, CarE, CAT, SOD, and POD are inhibited following the exposure to thiacloprid at one or more sample times and then increased during the recovery course compared with the control. Significant DNA damage to E. fetida was also observed by olive tail moments in comet assay. These results suggested that thiacloprid could have harmful effect on earthworms, and these studied biomarkers might be used in the assessment of the risk of thiacloprid to the soil ecosystem environment.

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (31171878 and 31272079).

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The authors declare that they have no conflict of interest.

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    Authors and Affiliations

    1. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China

      Lei Feng, Lan Zhang, Yanning Zhang, Pei Zhang & Hongyun Jiang

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    1. Lei Feng
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    2. Lan Zhang
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    3. Yanning Zhang
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    Correspondence to Hongyun Jiang.

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    Responsible editor: Henner Hollert

    Lei Feng and Lan Zhang contributed equally to this work.

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    Feng, L., Zhang, L., Zhang, Y. et al. Inhibition and recovery of biomarkers of earthworm Eisenia fetida after exposure to thiacloprid. Environ Sci Pollut Res 22, 9475–9482 (2015). https://doi.org/10.1007/s11356-015-4122-6

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