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Rapid determination of trace dicyandiamide in mussels from Zhejiang coast by ultra-fast liquid chromatography–tandem mass spectrometry with isotope internal standard dilution technique

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Abstract

In this study, a rapid and accurate ultra-fast liquid chromatography–tandem quadrupole mass spectrometry (UFLC–MS/MS) method coupled with the isotope internal standard dilution technique was established and validated to determine trace dicyandiamide (DCD) in mussels. The sample was extracted by acetonitrile, and chromatographic separations were performed on an Acquity UPLC BEH Amide column by using water–acetonitrile (9:91, v/v) as the mobile phase within 3 min. DCD was determined by using DCD-15N4 as an internal standard. The results showed that the recoveries were between 96.2 and 103 % with relative standard deviations (RSDs) in the range of 0.6–6.0 %. The limit of quantification (LOQ) was 0.05 μg/kg. This method can be applied to the routine analysis for the rapid and sensitive determination of trace DCD in mussels. Overall, the data reiterate the importance of investigating the presence of DCD in marine biological samples, which can act as food quality controls for human health.

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Acknowledgments

We would like to thank the Agriculture and Social Development Funds of Ningbo, China (No. 2011C50058); the Zhejiang Provincial Analytical Foundation of China (No. 2012C37002); and the Medical Science and Technology Funds of Ningbo (No. 2011A07).

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Authors and Affiliations

  1. Quzhou Center for Disease Control and Prevention, Quzhou, 324000, Zhejiang, China

    Yun Zhang & Hua Zhou

  2. Cixi Municipal Center for Disease Control and Prevention, Ningbo, 315300, Zhejiang, China

    Wen-Jie Gong

  3. Zhejiang Provincial Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, 315010, Zhejiang, China

    Yong-Gang Zhao

  4. Ningbo Key Laboratory of Poison Research and Control, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, 315010, Zhejiang, China

    Yong-Gang Zhao

Authors
  1. Yun Zhang
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  2. Wen-Jie Gong
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  3. Yong-Gang Zhao
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  4. Hua Zhou
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Correspondence to Yong-Gang Zhao.

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Responsible editor: Philippe Garrigues

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Zhang, Y., Gong, WJ., Zhao, YG. et al. Rapid determination of trace dicyandiamide in mussels from Zhejiang coast by ultra-fast liquid chromatography–tandem mass spectrometry with isotope internal standard dilution technique. Environ Sci Pollut Res 21, 14219–14225 (2014). https://doi.org/10.1007/s11356-014-3170-7

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  • DOI: https://doi.org/10.1007/s11356-014-3170-7

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