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Metabolomics as a tool for understanding the evolution of Tabebuia sensu lato

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Abstract

Background

Plant systematic studies have changed substantially in the last years, stimulated by new strategies for phylogenetic studies. In this regard, chemistry data has been a useful tool for understanding plant phylogenetic relationships.

Objective

Our aim was to apply metabolomic approaches, followed by multivariate statistical analysis and dereplication of Tabebuia sensu lato species, and compare our results with classifications based on traditional taxonomy and molecular phylogeny. We also evaluated the application of metabolomics as a chemotaxonomic identification tool, as well as to enlighten plant chemical evolution.

Methods

Metabolomic data was generated through a high-resolution mass spectrometry with electrospray ionization of 27 Tabebuia sensu lato specimens from different populations, consisting of 15 Handroanthus (from four species) and 12 Tabebuia sensu stricto (from three species). Chemometric tools, such as principal component analysis and metabolite heatmaps, were used to scrutinize the metabolic changes among species.

Results

Tabebuia and Handroanthus species presented different secondary metabolite storage capacity. The genus Tabebuia revealed higher levels of glycosylated iridoids esterified with a phenylpropanoid moiety, such as specioside, verminoside, and minecoside, while Handroanthus accumulated iridoids linked to a simple phenol, lignans, and verbascoside derivatives.

Conclusion

These results corroborate splitting the Tabebuia s.l., which was supported by profound changes in secondary metabolism, suggesting metabolomics as an excellent tool for understanding species evolution.

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Acknowledgements

The authors would like to thank the Fundação de Apoio ao Desenvolvimento do Ensino, Ciência e Tecnologia do Estado de Mato Grosso do Sul (FUNDECT) and the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for the research grant given to authors, and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).

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  1. Laboratório de Produtos Naturais e Espectrometria de Massas (LaPNEM), Faculdade de Ciências Farmacêuticas, Alimentos e Nutrição (FACFAN), Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande, MS, 79070-900, Brazil

    Vanessa Samúdio dos Santos, Jean Silva do Vale, Denise Brentan Silva & Carlos Alexandre Carollo

  2. Laboratório de Botânica, Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande, MS, 79070-900, Brazil

    Flávio Alves Macedo

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  1. Vanessa Samúdio dos Santos
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  2. Flávio Alves Macedo
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Correspondence to Carlos Alexandre Carollo.

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dos Santos, V.S., Macedo, F.A., do Vale, J.S. et al. Metabolomics as a tool for understanding the evolution of Tabebuia sensu lato . Metabolomics 13, 72 (2017). https://doi.org/10.1007/s11306-017-1209-8

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